- Open Access
Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows
Journal of Animal Science and Biotechnology volume 11, Article number: 1 (2020)
The aim of the study was to investigate the effect of energy overfeeding during the dry period on adipose tissue transcriptome profiles during the periparturient period in dairy cows.
Fourteen primiparous Holstein cows from a larger cohort receiving a higher-energy diet (1.62 Mcal of net energy for lactation/kg of dry matter; 15% crude protein) for ad libitum intake to supply 150% (OVR) or 100% (CTR) of energy requirements from dry off until parturition were used. After calving, all cows received the same lactation diet. Subcutaneous adipose tissue (SAT) biopsies were collected at − 14, 1, and 14 d from parturition (d) and used for transcriptome profiling using a bovine oligonucleotide microarray. Data mining of differentially expressed genes (DEG) between treatments and due to sampling time was performed using the Dynamic Impact Approach (DIA) and Ingenuity Pathway Analysis (IPA).
There was a strong effect of over-feeding energy on DEG with 2434 (False discovery rate-corrected P < 0.05) between OVR and CTR at − 14 d, and only 340 and 538 at 1 and 14 d. The most-impacted and activated pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that were highlighted by DIA analysis at − 14 d in OVR vs. CTR included 9 associated with carbohydrate metabolism, with ‘Pyruvate metabolism’, ‘Glycolysis/gluconeogenesis’, and ‘Pentose phosphate pathway’ among the most-activated. Not surprisingly, OVR led to marked activation of lipid metabolism (e.g. ‘Fatty acid biosynthesis’ and ‘Glycerolipid metabolism’). Unexpected metabolic pathways that were activated at − 14 d in OVR included several related to metabolism of amino acids (e.g. branched chain) and of cofactors and vitamins (thiamin). Among endocrine and immune system pathways, at − 14 d OVR led to marked activation of ‘PPAR signalling’ and ‘Antigen processing and presentation’. Among key pathways affected over time in OVR, a number were related to translation (e.g. mTOR signaling), endocrine/immune signaling (CXCR4 and IGF1), and lipid metabolism (oxidative phosphorylation) with greater activation in OVR vs. CTR specifically at − 14 d. Although statistical differences for several pathways in OVR vs. CTR nearly disappeared at 1 and 14 vs. − 14 d, despite the well-known catabolic state of adipose depots after calving, the bioinformatics analyses suggested important roles for a number of signaling mechanisms at − 14 vs. 14 than 1 vs. -14 d. This was particularly evident in cows fed to meet predicted energy requirements during the dry period (CTR).
Data underscored a strong activation by overfeeding energy of anabolic processes in the SAT exclusively prepartum. The study confirmed that higher-energy diets prepartum drive a transcriptional cascade of events orchestrated in part by the activation of PPARγ that regulate preadipocyte differentiation and lipid storage in SAT. Novel aspects of SAT biology to energy overfeeding or change in physiologic state also were uncovered, including the role of amino acid metabolism, mTOR signaling, and the immune system.
The transition from pregnancy into lactation is characterized by dramatic and sudden physiological changes, and it is recognized to be the most difficult stage in the dairy cow’s life [1, 2]. During this period, metabolic adaptations in major organs (e.g., mammary, liver, rumen, and adipose) are coordinated to allow the animal to satisfy the needs for synthesizing milk. Although the concerted biological roles of liver and mammary during the transition period in coordination of animal physiology are well known , similar knowledge for adipose tissue activity is scant .
Adipose tissue is not simply a metabolic tissue that primarily participates in regulating whole body energy homeostasis, it also plays an important endocrine function (at least in non-ruminants) by secreting a number of proteins with signaling properties that are involved in the regulation of metabolism (adiponectin, leptin), feed intake (leptin), and immune function and inflammation [5, 6]. Despite the dominance of mature adipocytes, adipose tissue is also composed of immune cells (macrophages) and stromal-vascular cell fractions containing pre-adipocytes, endothelial cells, and mesenchymal stem cells, which may vary in their response to external stimuli (such as nutrient supply) and immune activation .
Recent work aimed at investigating transcriptomic adaptations of adipose tissue during the transition period revealed several changes in expression of genes involved in the regulation of lipid metabolism in particular, but also immune-related functions [4, 7, 8]. Among the factors that can modulate the success during the transition period, energy intake is one of the most-studied [9,10,11]. In that context, adipose tissue appears to be very sensitive to energy status of the organism . Energy overnutrition in the dry period and the resulting over conditioning of periparturient dairy cows  represent possible risk factors for optimal health status around calving.
Previous experiments reported that energy overfeeding in the prepartum is often associated with negative effects in postpartum health indices, underscoring possible detrimental effects of this nutritional approach [13, 14]. In particular, the negative effect of prepartum overfeeding causes greater postpartum adipose tissue mobilization, increased risks of ketosis and fatty liver postpartum [15,16,17]. Therefore, the objective of the present research was to study the effect of energy overnutrition during the dry period on the adipose tissue transcriptome during the transition period. A subset of cows from the study of Janovick and Drackley  were used for transcriptome profiling.
Materials and methods
All procedures were conducted under protocols approved by the University of Illinois Institutional Animal Care and Use Committee. The experimental design, management details and details of ingredients and nutrient composition of diets have been published previously [12, 15]. Briefly, 14 cows (7 per treatment group) from the larger cohort entering their first lactation were randomly selected for adipose tissue biopsy. All cows were moved to individual ties stalls at 65 d before expected parturition and were fed the herd dry cow diet. From 42 d before expected parturition to parturition, cows were assigned to a high-energy diet fed for ad libitum intake (OVR) to provide at least 150% of NRC energy requirements for dry cows in late gestation, or to a control-energy diet (CTR) to limit energy intake to 100% of NRC requirements at ad libitum intake . The latter was achieved using chopped wheat straw at 31.8% of the DM (Table 1). Initial average body condition score was 3.58 and 3.50 (5-point scale) and body weight 569 and 592 kg for CTR and OVR. Cows remained on their respective treatments until parturition. The same lactation diet was fed to all cows during lactation (Table 1).
Adipose tissue biopsy and handling
Subcutaneous adipose tissue biopsies were collected from alternate sides of the tail-head region at − 14, 1, and 14 d from parturition, before the morning feeding. The hair of the surgical area was cut closely with clippers and washed with an iodine disinfectant mixture. Lidocaine-HCl (5 mL; Agri Laboratories) was given intramuscularly to anaesthetize the biopsy area 10 min before performing a ~ 2-cm incision. Adipose tissue (2–4 g) was collected with scalpel and forceps by blunt dissection. The incision was then closed with surgical staples (Multi-Shot Disposable Skin Stapler, Henry Schein) and iodine ointment was applied to the wound. The wound was carefully monitored for the following 7 d. The tissue was quickly blotted with sterile gauze to remove residual blood and snap-frozen in liquid N until RNA extraction for gene expression analysis.
RNA extraction and microarrays analysis
Procedures for RNA extraction and microarray analysis have been described previously . The transcript profiling was done using a bovine oligonucleotide (70-mer) microarray with > 13,000 annotated sequences developed at the University of Illinois . Details of the development, annotation, use of this microarray, and methods for microarray hybridization and scanning have been reported previously .
Microarray spots with median intensity ≥3 standard deviation above the median of the background and GenePix 6 flag > 100 were applied as filters to ensure high quality data. A total of 106 microarrays were adjusted for dye and array effect (Loess normalization and array centering), duplicated spot intensities were not averaged and were subsequently used for statistical analysis. A mixed model with repeated measures was then fitted to the normalized log2-transformed adjusted ratios (sample/reference standard) using Proc MIXED (SAS, SAS Inst. Inc., Cary, NC). The model included the fixed effects of time (− 14, 1, 14 d), diet (OVR and CTR), and interactions of time × diet. Cow was considered as a random effect. The P-values were adjusted for the number of genes tested using Benjamini and Hochberg’s false discovery rate (FDR) to account for multiple comparisons . Differences in relative gene expression were considered significant at an FDR-adjusted P ≤ 0.05 for time × diet. A P ≤ 0.05 was considered significant between diets at each time point.
Ingenuity pathway analysis
Ingenuity Pathway Analysis (IPA, Ingenuity Systems) was used to identify enriched pathways in each comparison. The whole annotated dataset with FDR, expression ratio, and P-value for each comparison was uploaded in IPA. The whole annotated dataset was used as background and the default databases in IPA were used. IPA was also used to identify up-stream regulators as described previously . Network analysis was also performed using IPA.
Dynamic impact approach
The Dynamic Impact Approach (DIA) was used to identify impact and direction of the impact in KEGG pathways determined by DEG as previously described . Only pathways with at least 4 genes present in the annotated microarray were considered.
The number of differentially expressed genes (DEG) according to up-regulation or down-regulation are displayed in Table 2. The complete dataset is available in Additional file 1. The transcriptome during the transition from pregnancy to lactation was more affected in OVR compared with CTR. We detected a large number of DEG between OVR and CTR at − 14 d with a total of 2434 DEG. After calving, the number of DEG between OVR and CTR was lower with only 340 and 538 DEG at 1 and 14 d, respectively.
Impact of transition into lactation
Figure 1 depicts the pathway summary generated by DIA analysis for the impact value and the direction of the impact for the major KEGG pathway categories. Details are available for all the pathways in Additional file 2. Figures 2 and 3 depict the direction of impact for the most impacted pathways. There was a lesser impact of change in stage of lactation in CTR compared with OVR cows. Pathways related to metabolism were the most impacted in all comparisons.
Among metabolic-related pathways, the most impacted in the CTR group were ‘Biosynthesis of other secondary metabolites’ driven by ‘Caffeine metabolism’, which was activated after calving, and ‘Xenobiotics biodegradation and metabolism’ driven by P450 enzyme-related pathways that was inhibited during lactation (Fig. 1). With a minor impact in CTR among categories of pathways, we detected an activation of ‘Translation’ and an inhibition of ‘Metabolism of other amino acids’, especially ‘Phe, Tyr and Trp biosynthesis’.
In OVR cows, the DIA analysis revealed a large number of highly impacted categories of KEGG pathways during the transition from the dry period to lactation; especially at 1 vs. −14 d (Fig. 1). There was an overall inhibition of all pathways during the transition into lactation. Among categories of pathways, the most inhibited were related to metabolism including carbohydrate and lipid. Especially inhibited were pathways related to synthesis of triglycerides via use of glucose, such as pyruvate metabolism and TCA cycle, with ‘Fatty acid biosynthesis’ as the top-affected pathway. The second most inhibited category of pathways was ‘Cellular processes’, where pathways related to catabolism and cell proliferation were the most-affected (Fig. 1 and Additional file 2). A marked inhibition of metabolism and cell proliferation in OVR was detected only during the last 2 weeks of pregnancy while during the first 2 weeks of lactation (i.e., 14 vs. 1 d) (with the exception of “Biosynthesis of Other Secondary Metabolites”) most of the same pathways were induced. Besides metabolic-related pathways, also translation, protein degradation, transport and catabolism, cellular communication, and endocrine systems were strongly inhibited. In the latter categories of pathways, the most affected was ‘PPAR signaling pathway’. This pathway was slightly affected in CTR cows, but was among the most different between the two groups at − 14 d and one of the most inhibited in OVR from − 14 to 1 d, together with pathways related to triglyceride synthesis (Fig. 2 and Additional file 2).
Most affected pathways between CTR and OVR at − 14 d are reported in Fig. 3. Compared with CTR, before calving the OVR group had higher activation of pathways involved in carbohydrate metabolism including ‘Pyruvate metabolism’, ‘Propanoate metabolism’, ‘Glycolysis/gluconeogenesis’, ‘Citrate cycle (TCA cycle)’, and ‘Pentose phosphate pathway’. At the same time, compared with CTR, pathways related to lipid metabolism had a higher degree of activation (Fig. 3). Among the most activated pathways were those related to triglyceride synthesis including ‘Fatty acid biosynthesis’, ‘Biosynthesis of unsaturated fatty acids’, and ‘Glycerolipid metabolism’ (Fig. 3). In addition, prior to parturition, amino acid-related pathways were more activated in OVR compared with CTR; those include ‘Glutathione metabolism’, ‘Valine, leucine and isoleucine degradation’, ‘Arginine and proline metabolism’, and ‘Thiamine metabolism’. Besides metabolic-related pathways, also translation (i.e., ‘Ribosome’), especially including proteins formed in the ER, ‘Antigen processing and presentation’ among immune-related pathways, and ‘PPAR signaling’ were more activated in OVR compared with CTR (Fig. 3 and Additional file 2).
Ingenuity pathway analysis of enriched pathways
The analysis using Ingenuity Pathway Analysis confirmed the importance of translation and lipid metabolism in adipose tissue during the transition from pregnancy to lactation (Fig. 4 and Additional file 3). In both CTR and OVR, the DEG affected by time were highly enriched with genes related to signaling pathways, translation, and lipid metabolism. In addition, genes with different expression between OVR and CTR were highly enriched with pathways related to signaling, including the mTOR pathway. Pathways related to lipid metabolism were also enriched; however, enriched were pathways involved in energy production using lipids.
Ingenuity pathway analysis of transcriptional regulators
The analysis using Ingenuity Pathway Analysis revealed few upstream regulators deemed important in regulating the transcription of DEG detected in this study with an estimated Z-score ≥ 2 (i.e., activated) or ≤ − 2 (i.e., inhibited) (Fig. 5). In particular, TNF was estimated to be inhibited in adipose tissue of OVR compared with CTR cows in the pre-partum at − 14 d (Fig. 5). Genes related to the inflammatory cascade, CCL5 (involved in immunoregulatory and inflammatory process), and PTGS2 (key enzyme in prostaglandin biosynthesis) were downregulated by the inhibition of TNF (Fig. 6). Furthermore, the inhibition of TNF led to the upregulation of genes related to fatty acid synthesis, ACACA (catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis) and FASN (which catalyze the synthesis of long-chain saturated fatty acids). The latter was the most upregulated gene (Fig. 6).
Except for cytokines such as TNF and IL1B, all upstream regulators in both CTR and OVR were estimated to be inhibited in adipose tissue during the transition from pregnancy to lactation. The most important inhibited upstream molecules were the nuclear receptor PPARγ and the growth factor FGF21 and ghrelin (GHRL). These also were among the most induced in OVR and CTR animals at − 14 d. These upstream regulators form a tight gene network (Fig. 6). It is noteworthy to highlight that in the network displayed in Fig. 6, several genes related to insulin signaling (i.e., IRS1) and glucose uptake (i.e., SLC2A4) were down-regulated, in OVR vs. CTR at − 14 d despite being PPARγ target genes.
Few studies have evaluated changes in adipose tissue during the transition period, but they have revealed a dramatic transcriptome downregulation after parturition with a quick interruption of many anabolic functions related to lipogenesis [4, 22]. On the basis of gene expression data generated by RT-qPCR, overfeeding high yielding dairy cows in the prepartal period resulted in slight alterations of the transcriptional adaptations of crucial genes in subcutaneous adipose tissue and did not compromise the insulin signaling pathway [7, 16]. However, the negative consequences of overfeeding energy during the dry period are manifested at the beginning of the new lactation when the energy stored previously as fat is mobilized.
In the present study, the subcutaneous adipose tissue was more affected by energy content of the diet than the physiological change at the onset of lactation. This was evidenced by the relatively low number of DEG during the transition in cows fed as recommended by the NRC , while cows fed a higher energy diet prepartum (150% of requirement) had almost 3000 DEG during the transition into lactation. Furthermore, despite all the cows receiving the same diet in the postpartum, the two groups had more than 500 genes with a different expression and the number of DEG was persistently greater in OVR vs. CTR cows also in the postpartum. These data underscore a carryover effect of the higher energy diet on the adipose tissue transcriptome. The effects on the transcriptome detected in our study partly support prior observations, and in particular a large and persistent effect of energy intake on lipogenesis [23,24,25].
The bioinformatics analysis of our data clearly indicated a strong lipogenic effect of the higher energy diet in the prepartum. This confirms that lipogenesis is mainly regulated at a transcriptional level (as in non-ruminants), while lipolysis, which is predominant during negative energy balance is mainly controlled through other mechanisms, e.g., post-translational activation by protein phosphorylation . After the beginning of lactation all cows received the same diet and differences between OVR and CTR were strongly diminished, suggesting a relatively quick homeorhetic adaptation to dietary energy level, allowing its partitioning at the onset of lactation.
The diet received by the OVR cows in our experiment contained a higher amount of fermentable carbohydrate (corn grain: 17.9% in OVR vs. 3.6% in CTR). This would stimulate the production and absorption of ruminal VFA  and increase glucose availability ; hence, increasing the supply of energy that in turn results in the stimulation of lipogenesis . The principal effect of changing from a conventional diet to a higher-energy diet is that propionic acid production and net energy from total VFA increase . Overall, the greater biosynthesis of fatty acids from increased VFA uptake by adipose tissue of OVR compared with CTR cows is supported by the larger induction of the ‘Fatty acid biosynthesis’ pathway. Other data supporting a positive effect on lipogenesis by OVR is the strong upregulation of acetyl-CoA synthetase (ACSS; 7.4-fold higher in OVR vs. CTR at − 14 d) the enzyme catalyzing the synthesis of acetyl-CoA from acetate. We speculate that through changes in that enzyme, large amounts of acetate were absorbed from adipose tissue and used for de novo fatty acid synthesis [29, 30]. The higher synthesis of fatty acids is also supported by the greater induction in OVR vs. CTR of pathways related to utilization of glucose, including the pentose phosphate pathway responsible for the production of NADPH, and production of pyruvate that enters the TCA cycle producing intermediates such as citrate . The latter can produce acetyl-CoA via catalysis of ATP citrate lyase (ACLY) allowing glucose and, to a lesser extent, lactate carbon to serve as substrates for fatty acid synthesis.
The greater mRNA abundance of lipogenic genes by energy overfeeding in the present study might have been induced by a combination of greater substrate supply and insulin, which is a well-known lipogenic hormone . The inclusion of ground corn in OVR compared with chopped wheat straw in the CTR diet  potentially provided greater amounts of substrates (acetate from ruminal fermentation and glucose derived mostly from gluconeogenesis from propionate) for lipogenesis. Despite the lack of difference in blood glucose concentration prepartum between treatments , the prepartum serum insulin concentration was greater and NEFA lower in OVR compared with CTR  confirming a greater anabolic status as previously discussed by Piccioli-Cappelli et al. .
The overall results described suggest a model where the greater energy intake before parturition drives a transcriptional cascade regulating pre-adipocyte differentiation (adipogenesis) and adipocyte function mainly related to energy storage. Hundreds of genes including enzymes and transcription factors coordinate the expression of proteins responsible for establishing the mature adipocyte. The central event in this network is the activation of CCAAT enhancer binding proteins (CEBP) and PPARγ, which are essential transcription regulators for the entire process [33,34,35]. In particular, PPARγ, highly expressed in bovine adipose tissue , is considered the master regulator of adipogenesis  and appears to control lipogenesis in response to energy level in the diet [16, 37, 38]. In non-ruminants, PPARγ controls the induction of C/EBPα , whereas the expression of C/EBPα appears to be required for maintaining expression of PPARγ in the mature fat cell . Besides lipogenesis, PPARγ might also play a role in fatty acid oxidation  controlling the expression of carnitine palmitoyl transferase 2 (CPT2; upregulated 1.7-fold in OVR vs. CTR cows in the present study), a protein involved in the entry of long chain fatty acids into the mitochondria prior to their oxidation. Despite a greater induction of lipogenesis in OVR vs. CTR at − 14 d, our data also indicated a larger induction of fatty acid metabolism, including catabolism.
A noteworthy aspect related to PPARγ is its impact on insulin resistance. The treatment with PPARγ agonists is a clinical approach used to treat insulin resistance. Insulin insensitivity in peripheral tissues during the transition period is an important homeorhetic adaptation . In our study, the increased expression of PPARG and the consequent effect on several target genes in OVR cows compared with CTR may be considered a homeorhetic mechanism that acts to balance the otherwise greater insulin resistance in overfed dairy cows . We previously proposed that the increased abundance and activation pre-partum of PPARG in adipose tissue can help alleviate the large NEFA surge due partly to the control it exerts on transcription of the insulin-sensitive glucose transporter (SLC2A4) plus other genes favoring lipogenesis and esterification (e.g. FASN, PCK1). This would lead to reduced lipid overload on the liver with a consequent decrease in susceptibility to lipidosis and other potential detrimental effects on metabolic health .
One of the most-novel outcomes from the present study was the discovery of alterations in metabolic pathways related to amino acid metabolism in OVR cows. For instance, we observed an activation of branched-chain amino acid (BCAA) catabolism, the increase of which (at least in non-ruminants) is coordinated by PPARγ and is essential to support adipocyte differentiation and lipogenesis [43, 44]. In addition to their role as indispensable components for life, the BCAA valine, leucine, and isoleucine (most-abundant of the circulating essential amino acids) are key regulators of protein synthesis, protein degradation, and insulin secretion and synthesis . In mammals, the BCAA are initially transaminated by branched chain amino transferases (BCAT) to form branched chain α-ketoacids (BCKA), representing the first step of BCAA catabolism. The last step of BCAA catabolism (all reactions occur within the mitochondrial matrix) provide carbon skeletons that are either lost as CO2 or enter the TCA cycle. Furthermore, BCAA metabolism contributes to the synthesis of several lipid species including branched chain fatty acids, odd-chain fatty acids, and N-acyl amino acids. Indeed, adipocytes (particularly) can synthesize odd-chain fatty acids by combining propionyl-CoA (carbon derived from valine and isoleucine) and malonyl-CoA, followed by fatty chain elongation via fatty acid synthase [43, 46].
Although there are no published data in ruminants to help understand the specific connection between activation of BCAA degradation and activation of lipid metabolism-related pathways as direct consequence of energy overfeeding, there are some in vitro studies for such a connection. Crown et al.  using cultured 3 T3-L1 adipocytes where the medium was supplemented with radiolabeled valine, leucine and isoleucine demonstrated that at least 25% of lipogenic acetyl-CoA was derived from BCAA catabolism (leucine and isoleucine). Similarly, propionyl-CoA, precursor for odd chain fatty acids, was derived solely from isoleucine and valine (accounting for 100%), confirming the relevant contribution of BCAA to lipogenesis in differentiated adipocytes.
The activation of the ‘Valine, leucine and isoleucine degradation’ pathway implies there was greater availability of these BCAA from dietary uptake or ruminal microbiota synthesis. Compared with CTR, the greater dietary energy level in OVR cows likely improved the efficiency of microbial N synthesis. Indeed, microbial N flow to the duodenum is improved at low dietary CP content when changing from higher-fiber to higher-starch diets [47, 48]. Thus, enriching diets with highly-fermentable grain (current study: 17.9% in OVR vs. 3.6% in CTR) in the prepartum period (normally characterized by low CP and higher fiber content) leads to greater microbial N flow to the duodenum and posthepatic availability of AA . In addition, the present study highlights that BCAA catabolism together with other AA-related pathways were inactivated at both 1 and 14 d compared with − 14 d. We speculate that this pattern changed following parturition likely due to the inactivation of lipid metabolism (such as fatty acid biosynthesis and PPAR signaling pathway), pyruvate metabolism, and TCA cycle. As such, the requirements of lipogenic intermediates (i.e. acetyl-CoA and propionyl-CoA) decreased, but also there was a shift in post-hepatic AA utilization from adipose tissue (prepartum) to mammary gland for milk production (postpartum). Recent data underscored that protein abundance of branched chain ketoacid dehydrogenase kinase (BCKDK) did not change between pre and postpartum periods in adipose tissue of periparturient Holstein cows, suggesting it is a response that helps channel circulating BCAA to the mammary gland .
In non-ruminants, adipose tissue also acts as endocrine organ and cross talks with other tissues by secretion of molecules including cytokines . In particular, the adipose tissue synthesizes and secretes circulating hormones and adipokines that act as systemic inflammatory mediators and signals of the organism’s nutritional status . Although transcriptional regulation of adipose by energy availability is well known in non-ruminants, such regulation in cow adipose tissue remains poorly understood. In this respect, with the application of bioinformatics analysis of the transcriptome data from adipose tissue of Holstein cows, Moisá et al.  shed light on changes of mRNA expression profiles of both mesenteric and subcutaneous adipose tissue when cows were fed a higher-energy diet. Data indicated that subcutaneous adipose tissue gene transcription responds more strongly to level of dietary energy, e.g. changes in mRNA abundance encompass not only metabolic pathways but also those involved in the regulation of immune function and inflammation. Although increased secretion of pro-inflammatory cytokines (e.g., TNFα, IL-1β, IL-6) due to obesity is a well-known mechanism associated with systemic chronic low-grade inflammation, reduced appetite, fatty liver disease, and insulin resistance in non-ruminants [52, 53], we did not detect differences in abundance of pro-inflammatory cytokines (TNF, IL1B and IL6) between dietary treatments. On the contrary, the upstream regulators analysis revealed an inhibition of TNF before parturition in OVR cows, which had a greater increase in BCS during the dry period .
The present findings of a weak pro-inflammatory response in adipose to higher-energy feeding are supported by a recent in vitro study by Lopreiato et al.  who challenged subcutaneous adipose tissue with bovine recombinant TNF. Furthermore, that study linked the response to greater expression of PPARG which in non-ruminants plays a fundamental regulatory role in the attenuation and counter regulation of inflammatory phenomena in adipose tissue. Based on in vitro data, activation of PPARγ can attenuate the negative metabolic effects of TNF-α on adipocytes, preventing a decrease in insulin-mediated glucose uptake . It is unclear what role (if any) the localized adipose inflammatory response may exert over the genesis of inflammatory conditions typical of the transition period, especially after parturition, in dairy cows [55, 56].
Summary and conclusions
Overfeeding energy during the dry period channels large amounts of energy substrates (e.g. acetate and propionate) to the adipose tissue for storage as triacylglycerol. The transcriptomic approach allowed us to uncover that the adipose tissue responds rapidly to overfeeding mainly under mechanisms of transcriptional control through activation of genes involved in lipid accumulation and amino acid metabolism. In this respect, the nuclear receptor PPARγ acts as one of the main players controlling transcription of genes involved in lipogenesis, but also coordinates catabolism of amino acids that likely become essential for promoting adipocyte differentiation and lipogenesis. Instead, despite energy overfeeding during the dry period, the early postpartum period is characterized by a complete loss of lipogenic gene transcription, where lipolysis appears mainly controlled through other mechanisms, e.g., post-translational activation by protein phosphorylation. As a physiological consequence, the carryover effect of overfeeding energy prepartum affects metabolism drastically, leading to higher NEFA and BHB levels coupled with greater degree of BCS loss (Fig. 7). However, these results underscore the fact that the “detrimental” effects of prepartum overfeeding (discerned from plasma concentrations of energy balance biomarkers) does not necessarily correlate with transcriptional changes in adipose. Lastly, these results underscore the key role of a controlled plan of energy nutrition prepartum, in particular avoiding excess intake of nonstructural carbohydrates, which in turn could increase the likelihood of metabolic disorders in the early post-partum.
Availability of data and materials
The datasets during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Drackley JK. ADSA Foundation scholar award. Biology of dairy cows during the transition period: the final frontier? J Dairy Sci. 1999;82:2259–73.
Knegsel A. Metabolic adaptation during early lactation: key to cow health, longevity and a sustainable dairy production chain. CAB Rev Perspect Agric Vet Sci Nutr Nat Resour. 2014;9. https://doi.org/10.1079/PAVSNNR20149002.
Loor JJ, Bionaz M, Drackley JK. Systems physiology in dairy cattle: nutritional genomics and beyond. Annu Rev Anim Biosci. 2013;1:365–92.
Sumner-Thomson JM, Vierck JL, McNamara JP. Differential expression of genes in adipose tissue of first-lactation dairy cattle1. J Dairy Sci. 2011;94:361–9.
Hauner H. The new concept of adipose tissue function. Physiol Behav. 2004;83:653–8.
Lee D-E, Kehlenbrink S, Lee H, Hawkins M, Yudkin JS. Getting the message across: mechanisms of physiological cross talk by adipose tissue. AJP Endocrinol Metab. 2009;296:E1210–29.
Selim S, Salin S, Taponen J, Vanhatalo A, Kokkonen T, Elo KT. Prepartal dietary energy alters transcriptional adaptations of the liver and subcutaneous adipose tissue of dairy cows during the transition period. Physiol Genomics. 2014;46:328–37.
Weber M, Locher L, Huber K, Kenéz Á, Rehage J, Tienken R, et al. Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: the adipokines apelin and resistin and their relationship to receptors linked with lipolysis. J Dairy Sci. 2016;99:1549–59.
Loor JJ, Dann HM, Guretzky NAJ, Everts RE, Oliveira R, Green CA, et al. Plane of nutrition prepartum alters hepatic gene expression and function in dairy cows as assessed by longitudinal transcript and metabolic profiling. Physiol Genomics. 2006;27:29–41.
Mukesh M, Bionaz M, Graugnard DE, Drackley JK, Loor JJ. Adipose tissue depots of Holstein cows are immune responsive: inflammatory gene expression in vitro. Domest Anim Endocrinol. 2010;38:168–78.
Graugnard DE, Bionaz M, Trevisi E, Moyes KM, Salak-Johnson JL, Wallace RL, et al. Blood immunometabolic indices and polymorphonuclear neutrophil function in peripartum dairy cows are altered by level of dietary energy prepartum. J Dairy Sci. 2012;95:1749–58.
Janovick NA, Drackley JK. Prepartum dietary management of energy intake affects postpartum intake and lactation performance by primiparous and multiparous Holstein cows. J Dairy Sci. 2010;93:3086–102.
Dann HM, Morin DE, Bollero GA, Murphy MR, Drackley JK. Prepartum intake, postpartum induction of ketosis, and Periparturient disorders affect the metabolic status of dairy cows. J Dairy Sci. 2005;88:3249–64.
Dann HMM, Litherland NBB, Underwood JPP, Bionaz M, D’Angelo A, McFadden JWW, et al. Diets during far-off and close-up dry periods affect Periparturient metabolism and lactation in multiparous Cows1. J Dairy Sci. 2006;89:3563–77.
Janovick NA, Boisclair YR, Drackley JK. Prepartum dietary energy intake affects metabolism and health during the periparturient period in primiparous and multiparous Holstein cows. J Dairy Sci. 2011;94:1385–400.
Ji P, Osorio JS, Drackley JK, Loor JJ. Overfeeding a moderate energy diet prepartum does not impair bovine subcutaneous adipose tissue insulin signal transduction and induces marked changes in peripartal gene network expression. J Dairy Sci. 2012;95:4333–51.
Shahzad K, Bionaz M, Trevisi E, Bertoni G, Rodriguez-Zas SL, Loor JJ. Integrative Analyses of Hepatic Differentially Expressed Genes and Blood Biomarkers during the Peripartal Period between Dairy Cows Overfed or Restricted-Fed Energy Prepartum. Kaderali L, editor. PLoS One. 2014;9:e99757.
Timpka T, Eriksson H, Gursky EA, Strömgren M, Holm E, Ekberg J, et al. Requirements and design of the PROSPER protocol for implementation of information infrastructures supporting pandemic response: a nominal group study. PLoS One. 2011;6:e17941 Available from: http://www.ncbi.nlm.nih.gov/pubmed/21464918.
Loor JJ, Everts RE, Bionaz M, Dann HM, Morin DE, Oliveira R, et al. Nutrition-induced ketosis alters metabolic and signaling gene networks in liver of periparturient dairy cows. Physiol Genomics. 2007;32:105–16.
Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. Source J R Stat Soc Ser B. 1995;57:289–300.
Moyes KM, Sørensen P, Bionaz M. The Impact of Intramammary Escherichia coli Challenge on Liver and Mammary Transcriptome and Cross-Talk in Dairy Cows during Early Lactation Using RNAseq. PENA i SUBIRÀ RN, editor. PLoS One. 2016;11:e0157480.
Khan MJ, Hosseini A, Burrell S, Rocco SM, McNamara JP, Loor JJ. Change in subcutaneous adipose tissue metabolism and gene network expression during the transition period in dairy cows, including differences due to sire genetic merit1. J Dairy Sci. 2013;96:2171–82.
McNamara JP. Regulation of bovine adipose tissue metabolism during lactation. 5. Relationships of lipid synthesis and lipolysis with energy intake and utilization. J Dairy Sci. 1989;72:407–18.
Sumner JM, McNamara JP. Expression of lipolytic genes in the adipose tissue of pregnant and lactating Holstein dairy cattle. J Dairy Sci. 2007;90:5237–46.
Rocco SM, McNamara JP. Regulation of bovine adipose tissue metabolism during lactation. 7. Metabolism and gene expression as a function of genetic merit and dietary energy intake. J Dairy Sci. 2013;96:3108–19.
Sutton JD, Dhanoa MS, Morant SV, France J, Napper DJ, Schuller E. Rates of production of acetate, propionate, and butyrate in the rumen of lactating dairy cows given Normal and low-roughage diets. J Dairy Sci. 2003;86:3620–33.
Reynolds CK, Dürst B, Lupoli B, Humphries DJ, Beever DE. Visceral tissue mass and rumen volume in dairy cows during the transition from late gestation to early lactation. J Dairy Sci. 2004;87:961–71.
Baldwin RL, McLeod KR, McNamara JP, Elsasser TH, Baumann RG. Influence of abomasal carbohydrates on subcutaneous, omental, and mesenteric adipose lipogenic and lipolytic rates in growing beef steers. J Anim Sci. 2007;85:2271–82.
Hanson RW, Ballard FJ. The relative significance of acetate and glucose as precursors for lipid synthesis in liver and adipose tissue from ruminants. Biochem J. 1967;105:529–36.
Smith SB, Crouse JD. Relative contributions of acetate, lactate and glucose to lipogenesis in bovine intramuscular and subcutaneous adipose tissue. J Nutr. 1984;114:792–800.
Vernon RG. Lipid metabolism in the adipose tissue of ruminant animals. Prog Lipid Res. 1980;19:23–106.
Sul HS, Latasa MJ, Moon Y, Kim KH. Regulation of the fatty acid synthase promoter by insulin. J Nutr. 2000;130:315S–20S.
Spiegelman BM, Hu E, Kim JB, Brun R. PPARγ and the control of adipogenesis. Biochimie. 1997;79:111–2.
Monaco E, Bionaz M, Rodriguez-Zas S, Hurley WL, Wheeler MB. Transcriptomics Comparison between Porcine Adipose and Bone Marrow Mesenchymal Stem Cells during In Vitro Osteogenic and Adipogenic Differentiation. Beltrami AP, editor. PLoS One. 2012;7:e32481.
Bionaz M, Monaco E, Wheeler MB, Ning H, Lin G, Lue T. Transcription Adaptation during In Vitro Adipogenesis and Osteogenesis of Porcine Mesenchymal Stem Cells: Dynamics of Pathways, Biological Processes, Up-Stream Regulators, and Gene Networks. Li W-J, editor. PLoS One. 2015;10:e0137644.
Bionaz M, Chen S, Khan MJ, Loor JJ. Functional role of PPARs in ruminants: potential targets for fine-tuning metabolism during growth and lactation. PPAR Res. 2013;2013:684159.
Loor JJ, Bionaz M, Invernizzi G. Systems Biology and Animal Nutrition: Insights from the Dairy Cow during Growth and the Lactation Cycle. Oxford: Wiley-Blackwell; 2011. p. 215–45.
Lopreiato V, Hosseini A, Rosa F, Zhou Z, Alharthi A, Trevisi E, et al. Dietary energy level affects adipose depot mass but does not impair in vitro subcutaneous adipose tissue response to short-term insulin and tumor necrosis factor-α challenge in nonlactating, nonpregnant Holstein cows. J Dairy Sci Am Dairy Sci Assoc. 2018;101:10206–19.
Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, et al. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell. 1999;4:611–7.
Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, Troy AE, et al. Cross-regulation of C/EBPα and PPARγ controls the transcriptional pathway of Adipogenesis and insulin sensitivity. Mol Cell. 1999;3:151–8.
Sharma S, Sun X, Rafikov R, Kumar S, Hou Y, Oishi PE, et al. PPAR-γ regulates carnitine homeostasis and mitochondrial function in a lamb model of increased pulmonary blood flow. PLoS One. Public Libr Sci; 2012;7:e41555.
Bell AW, Bauman DE. Adaptations of glucose metabolism during pregnancy and lactation. J Mammary Gland Biol Neoplasia. 1997;2:265–78.
Green CR, Wallace M, Divakaruni AS, Phillips SA, Murphy AN, Ciaraldi TP, et al. Branched-chain amino acid catabolism fuels adipocyte differentiation and lipogenesis. Nat Chem Biol. 2016;12:15–21.
Lackey DE, Lynch CJ, Olson KC, Mostaedi R, Ali M, Smith WH, et al. Regulation of adipose branched-chain amino acid catabolism enzyme expression and cross-adipose amino acid flux in human obesity. AJP Endocrinol Metab. 2013;304:E1175–87.
Neinast M, Murashige D, Arany Z. Branched chain amino acids. Annu Rev Physiol Ann Rev. 2019;81:139–64.
Crown SB, Marze N, Antoniewicz MR. Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes. Tochtrop GP, editor. PLoS One. 2015;10:e0145850.
Cantalapiedra-Hijar G, Lemosquet S, Rodriguez-Lopez JM, Messad F, Ortigues-Marty I. Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels. J Dairy Sci. 2014;97:5151–66.
Cantalapiedra-Hijar G, Peyraud JL, Lemosquet S, Molina-Alcaide E, Boudra H, Nozière P, et al. Dietary carbohydrate composition modifies the milk N efficiency in late lactation cows fed low crude protein diets. Animal. 2014;8:275–85.
Liang Y, Batistel F, Parys C, Loor JJ. Methionine supply during the periparturient period enhances insulin signaling, amino acid transporters, and mechanistic target of rapamycin pathway proteins in adipose tissue of Holstein cows. J Dairy Sci. 2019;102:4403–14.
Rosen ED, Spiegelman BM. Adipocytes as regulators of energy balance and glucose homeostasis. Nature. 2006;444:847–53.
Moisá SJ, Ji P, Drackley JK, Rodriguez-Zas SL, Loor JJ. Transcriptional changes in mesenteric and subcutaneous adipose tissue from Holstein cows in response to plane of dietary energy. J Anim Sci Biotechnol. 2017;8:85.
Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, et al. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest. 2003;112:1821–30.
Tilg H, Moschen AR. Insulin resistance, inflammation, and non-alcoholic fatty liver disease. Trends Endocrinol Metab. 2008;19:371–9.
Szalkowski D, White-Carrington S, Berger J, Zhang B. Antidiabetic thiazolidinediones block the inhibitory effect of tumor necrosis factor-alpha on differentiation, insulin-stimulated glucose uptake, and gene expression in 3T3-L1 cells. Endocrinology. 1995;136:1474–81.
Bertoni G, Trevisi E. Use of the liver activity index and other metabolic variables in the assessment of metabolic health in dairy herds. Vet Clin North Am Food Anim Pract. 2013;29:413–31.
Loor JJ, Bertoni G, Hosseini A, Roche JR, Trevisi E. Functional welfare – using biochemical and molecular technologies to understand better the welfare state of peripartal dairy cattle. Anim Prod Sci. 2013;53:931–53.
All procedures were conducted under protocols approved by the University of Illinois Institutional Animal Care and Use Committee.
Consent for publication
The authors declare that they have no competing interests.
Additional file 2. Complete result datasets generated by the DIA analysis from transcript profiling analysis in bovine subcutaneous adipose tissue from end of pregnancy (− 14 d) through early lactation (1 and 14 d) in dairy cow fed a control diet prepartum (CTR) or relatively high energy diet prepartum (OVR). The KEGG pathways are sorted by category and subcategory. Blue bars represent the Impact, while red and green bars depict the Direction of the Impact (red = upregulation, green = downregulation)
Additional file 3. Complete metabolic and non-metabolic pathways comparison generated by Ingenuity Pathway Analysis from transcript profiling analysis in bovine subcutaneous adipose tissue from end of pregnancy (− 14 d) through early lactation (1 and 14 d) in dairy cow fed a control diet prepartum (CTR) or relatively high energy diet prepartum (OVR).
About this article
Cite this article
Minuti, A., Bionaz, M., Lopreiato, V. et al. Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows. J Animal Sci Biotechnol 11, 1 (2020) doi:10.1186/s40104-019-0409-7
- Periparturient cow
- Prepartum overfeeding
- Subcutaneous adipose tissue