Fig. 3

Metabolic differences of adipose tissue at the end of pregnancy at − 14 d in dairy cows fed a control diet (CTR) or a higher-energy diet prepartum (OVR). Shown are outputs (i.e., direction of the impact and impact) of selected KEGG pathways from bioinformatics analysis using the Dynamic Impact Approach (DIA) and differentially expressed genes at − 14 d pre-partum for the most impacted pathways in the KEGG subcategory ‘Lipid metabolism’, ‘Carbohydrate metabolism’, ‘Amino acid metabolism’, and other selected pathways. The panels depict the impact (black line and dots) and the direction of the impact (bars; positive red bars denote activation while negative green bars inhibition) for the most impacted pathways in the KEGG subcategories