- Open Access
Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats
- Shoulong Deng†1,
- Kun Yu†2, 3,
- Wuqi Jiang2,
- Yan Li2,
- Shuotian Wang2,
- Zhuo Deng4,
- Yuchang Yao5,
- Baolu Zhang6,
- Guoshi Liu2,
- Yixun Liu1Email author and
- Zhengxing Lian2, 3Email authorView ORCID ID profile
© The Author(s). 2017
- Received: 9 August 2016
- Accepted: 12 December 2016
- Published: 9 January 2017
The Erratum to this article has been published in Journal of Animal Science and Biotechnology 2017 8:19
Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2 (TLR2) is important for host recognition of invading Gram-positive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in vivo.
We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria, triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1 (AP-1), phosphatidylinositol 3-kinase (PI3K) and transcription factor nuclear factor kappa B (NF-κB) and inflammation factors downstream of the signal pathway.
Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased mRNA levels of transcription factor NF-E2-related factor-2 (Nrf2) and the downstream gene, heme oxygenase-1 (HO-1). When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression level.
The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway.
- Haem oxygenase
- Nrf2 signal pathway
- Toll-like receptor 2
- Transgenic goats
Mastitis, an inflammatory and oxidative stress disease of udders, is caused by pathogenic bacteria. The disease is common in goats and other dairy animals. Mastitis reduces the quantity and quality of milk. Gram-positive bacteria such as staphylococcus, streptococcus and corynebacterium cause mastitis. Staphylococcus aureus is the main zoonotic agent of mastitis . During infection, the innate immune system of the host animal provides the first line of defense in pathogen recognition, and pattern recognition receptors (PRRs) are necessary for this process to occur. Toll-like receptor (TLR) 2 plays a critical role in recognition of the conserved portion of Gram-positive bacteria , and it is known that mastitis can increase expression of the TLR2 gene in mammary glands . Genetic diversity in the TLR2 gene is a risk factor for staphylococcus infection , and TLR2−/− mice are susceptible to S. aureus . The S. aureus cell wall components (e.g., peptidoglycan, lipoteichoic acid and lipoproteins) are recognizable by TLR2 . Pam3CSK4, a synthetic bacterial lipoprotein, is an agonist for TLR2 .
TLR2 is widely expressed in all types of goat immune cells and epithelial cells, and is highly expressed in peripheral blood monocytes. TLR2 activation relies mainly on the MyD88 signaling pathway, which activates the NF-κB pathway and mitogen-activated protein kinase (MAPK) pathway. Phosphorylated MAPK activates the transcription factor activator protein-1 (AP-1) and activation of the PI3K/Akt signaling pathway. Expression of inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, chemokines and nitrous oxide (NO) can all induce immune responses.
In mastitis, oxidative stress results from infection with pathogenic bacteria. Such infections in udders trigger host immune responses that induce production of large amounts of reactive oxygen species (ROS) in host cells . Transcription factor NF-E2-related factor-2 (Nrf2) plays a critical role in anti-inflammation and oxidation stress, induces anti-oxidation proteins and expression of the type II detoxification enzyme, and triggers expression of the downstream target genes catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase alpha 1 (GSTα1), quinone oxidoreductase 1 (NQO1), γ-glutamylcysteine synthetase (γ-GCS) and heme oxygenase-1 (HO-1). Some cellular signaling pathways such as PI3K and MAPK regulate activation of the Nrf2 antioxidative response element pathway . Oxidative stress activates NF-κB, triggers inflammatory responses, up-regulates expression of target genes such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and the oxidation enzyme NADPH, and facilitates prostaglandin, ROS and NO production, while intensifying the oxidative stress response. Crosstalk between Nrf2 and NF-κB pathways cooperatively regulates the oxidative stress response . HO-1 and its catalytic products enhance the innate protection system of the host . The HO-1 gene contains AP-1, NF-κB, Nrf2, which are regulatory structural sites. IL-10 and TLR pathways are involved in HO-1 regulation .
The expression level of TLR2 is closely associated with lipid oxidation and inflammation reactions , and also plays an important role in triggering innate and adaptive immune responses against infection, thereby protecting the host from disease. In this study, we over-expressed the TLR2 receptor in laso-shan dairy goats. Through stimulation with the Pam3CSK4 ligand from Gram-positive bacteria, we observed the effect of TLR2 on the immune response. We discuss the mechanism whereby TLR2 regulates anti-oxidative stress.
Production of transgenic goats over-expressing toll-like receptor 2
Isolation purification and culturing of goat monocytes-macrophages
Each group had 5 transgenic goats. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of 6-month-old transgenic goats using goat lymphocyte separation medium (TBD, Tianjin, China). The PBMCs containing thrombocytes, lymphocytes, and monocytes were washed twice with Hanks’ buffer (Invitrogen, Beijing, China). The harvested cells at a concentration of 2 × 107 cells/well were seeded in 6-well plate. The plates were incubated at 37 °C in an incubator with a 5% CO2 for 2 h and then the non-adherent cells (most of lymphocytes) were washed out with PBS. The adherent cells were cultured with RPMI1640 (Gibco, Grand Island, NY, USA) medium containing 10% FBS (Gibco). After 24 h of incubation and 3 times washing, the cells were digested with 0.02% EDTA for 5 min, and then the cells were harvested. The cells (1 × 105) were seeded in each well of a 6-well plate with three repeats for each group. RPMI1640 medium containing 10% FBS was changed every 24 h. After 48 h of incubating and washing away non-adherent cells, the adherent cells were mainly monocytes-macrophages [15, 16]. Monocytes-macrophages were stimulated by Pam3CSK4 (1 μg/mL) (InvivoGen, San Diego, CA, USA) . Cell suspensions were collected at 0, 1, 8 and 48 h post-stimulation. RNA was extracted from adherent cells using TRIzol plus RNA purification kit (Invitrogen, Carlsbad, CA, USA) and treated with RNase-free DNase (Promega, Madison, WI, U.S.) per the manufacturer’s instructions. cDNA was synthesized with a Reverse Transcription kit (Promega, Madison, WI, USA).
Reverse transcription (RT) and quantitative real-time (qRT)-PCR
The primer sequences
Enzyme-linked immunosorbent assays (ELISAs)
During the TLR2 receptor agonist experiments, cell culture supernatants from Pam3CSK4-stimulated monocytes-macrophages were collected at different time points (0, 1, 8, and 48 h). The concentrations of TLR2, TNF-α, IL-1β, IL-8, IL-12p40, IL-4 and monocyte chemoattractant protein-1 (MCP-1) in the supernatants were measured using ELISA kits (CUSABIO, Hubei, China). Sera were obtained to detect TLR2 concentration using ELISA kit. Blood samples were collected from goats and allowed to clot at 37 °C for 30 min. Sera were separated immediately by centrifugation at 3,000 r/min for 15 min. All experimental operations were performed according to the kit instructions.
Oxidative stress measurements
Pam3CSK4-stimulated monocytes-macrophages suspensions were collected at different time points (0, 1, 8, and 48 h). The activities of iNOS, SOD, CAT, GSH, COX-2, NADPH oxidase and malondialdehyde (MDA) contents were examined by spectrophotometry in accordance with the manual supplied with the detection kit (Nanjing Jiancheng, China).
The ears of three 8-month-old transgene-positive goats were injected intradermally with 100 μL of 3 mg/mL Pam3CSK4, after which the tissues were collected at 1, 8, and 48 h [18, 19]. Samples were fixed with 4% paraformaldehyde and embedded in paraffin. Hematoxylin and eosin staining was used to investigate inflammatory responses and immunohistochemistry was used to detect HO-1 protein expression (Abcam, ab13248, Cambridge, UK). Six fields from each slide were randomly selected. Optical densities were quantified by scanning densitometry and expressed in arbitrary units determined by Image J software (NIH, USA).
Individual experiments were repeated three times. Statistical tests to determine differences of TLR2 mRNA expression between two groups were performed with one-way ANOVA followed by Tukey’s HSD using Statistical Analysis System (SAS Institute, Cary, NC, USA). A repeated measures ANOVA with SAS proc GLM followed by Tukey’s HSD post-hoc tests were performed to determine the statistical significance between the relative groups in different time points. All data were expressed as mean ± SEM. Differences were considered to be significant when P < 0.05.
Toll-like receptor 2 over-expression triggers activation of down-stream transcription factors
Microinjection production of over-expression of TLR2 gene in goats
No. of donors
No. of microinjections
No. of embryo transfer recipients
No. of lambs
Southern positive rate, %
ELISA was performed to measure the TLR2 expression levels in the Pam3CSK4-stimulated monocytes-macrophages. TLR2 expression levels in the transgenic group were significantly higher than those of the Wt group at 1 and 8 h post-stimulation (P < 0.05) (Fig. 1e). These results confirmed that TLR2 expression of the monocytes-macrophages of the transgenic group was up-regulated after Pam3CSK4 stimulation. Next, qRT-PCR was conducted to determine NF-κB and AP-1 mRNA expression levels. In this experiment, NF-κB expression in the transgenic group was significantly higher than that in the Wt group at 1 h, and reduced at 8 h post-stimulation compared with Wt (Fig. 1f). These results show that TLR2 triggered activation of the NF-κB signal pathway, with NF-κB expression levels decreasing at an earlier stage than those of Wt. AP-1 expression in the transgenic group was significantly higher than that of the Wt group at 1 h post- stimulation, reached a peak at 8 h, while its expression in Wt group continued to increase over time (Fig. 1g). The expression levels of c-Fos, c-Jun and PI3K were similar as AP-1 (Fig. 1h, i, j and k).
Toll-like receptor 2 over-expression improves expression of the anti-inflammatory factor
Weakens oxidative lipid injury in monocytes-macrophages
Over-expressed toll-like receptor 2 retains anti-oxidative protein activity in goat monocytes-macrophages
Over-expressed toll-like receptor 2 up-regulates expression of the anti-oxidative gene heme oxygenase-1
Toll-like receptor 2 overexpression triggers infiltration of neutrophils and high-level expression of heme oxygenase-1 protein
Activated TLR2 triggers MyD88 protein, and I-κB kinase phosphorylation activates transcription factor NF-κB or alternatively, the MAPK pathway activates AP-1 to induce the expression of inflammatory cytokines. AP-1 is a pro-inflammation mediated transcription factor, the core components of which are c-Jun and c-Fos. Overexpression of c-Jun has been shown to induce production of inflammation mediators , while c-Jun/AP-1 interacts with the transcription factor, NF-κB. In cells under oxidation stress, TLR2 inhibition stopped transportation of NF-κB and AP-1 . We have obtained transgenic goats with TLR2 over-expression. Excessive expression of TLR2 mRNA was detected in monocytes-macrophages of the transgenic group. Transgenic group expressed more TLR2 protein than the Wt group in the ear tissue section . In goat sera, TLR2 protein levels of the transgenic group were higher too. However without stimulation, there was no significant difference in the expression of TLR2 protein between the transgenic group and the Wt group in monocytes-macrophages. This may be caused by the negative regulation of TLRs receptors . Previous study indicated, TLRs levels were kept in relative stable levels by intracellular modulation, such as alternative splice, degradation by ubiquitination and deubiquitination . After Pam3CSK4-stimulation of transgenic goat monocytes-macrophages, the expression of TLR2 protein was up-regulated in the transgenic group and triggered activation of downstream transcription factors, promoted the expression of AP-1 and inhibited the activity of NF-κB. Protein levels of TLR2 in different tissues were different, and further research on the different oxidative stress response caused by it will be studied.
TNF-α and IL-1β are both able to induce the production of MCP-1. In animal experiments, it has been shown that an increase in oxidation stress can induce the expression of MCP-1 . Inflammation was accompanied with an anti-inflammation reaction, and IL-4 played an important role in it. The Th1/Th2 balance is determined by two different types of cytokine secretion. The Th1 response produces IL-12 to promote the production of IFN-γ, whereas the Th2 response is distinguished by low IFN-γ levels and high IL-4 levels. TLR2 activated by different ligands generates different immune effects, and the expression of TLR2 shows time-dependence [25, 26]. It has been shown that stimulation by the TLR2 agonist Pam3CSK4 reduces the infiltration of chemokine and inflammatory cells within mouse tissues, in vivo . In the present study, Pam3CSK4 could activate the monocytes-macrophages, and overexpressed TLR2 caused early expression of the pro-inflammatory cytokines TNF-α and IL-1β, and a continuous increase in expression of the anti-inflammatory factor IL-4, as well as inducing expression of Th1 type cytokines. The result of our skin inflammation experiment indicated that over-expression of the TLR2 gene accelerated the inflammation process.
Under inflammatory conditions, free radicals are necessary for host defense. Inflammatory cells produce ROS and NO to expose the area of inflammation under oxidative stress. The transcription factors AP-1, NF-κB and Nrf2 all exhibit sensitivity to reductant-oxidant stressors. TLR2 activation of mouse macrophages produces NO resulting in damage to bacteria within cells , while NADPH oxidase regulates the production of ROS, over-expresses pro-inflammatory cytokines, and positively facilitates oxidative stress. Within cells, there is both elevation of ROS and activation of NF-κB. The production of NO is regulated by iNOS, and the activation of Nrf2 can reduce induction of iNOS expression by IL-1β and inhibit the activity of NF-κB . The results of the present study showed that Pam3CSK4 stimulated goat monocytes-macrophages, and over-expressed TLR2 up-regulated the activity of NADPH oxidase. Furthermore, the low activity of iNOS in the TLR2 over-expressed group showed that NO was synthesized in small amounts, and that the oxidative stress injury to cells was weak. Over-expressed TLR2 caused the over-expression of Nrf2 and inhibited iNOS activity. It has been shown that an Nrf2 agonist inhibited TNF-α and IL-1β and induced COX-2 expression by blocking the activation of NF-κB . High expression of COX-2 inhibited PI3K activity and the anti-oxidation reaction mediated by Nrf2 . In addition, activation of Nrf2 can regulate c-Jun signal activity directly, and COX-2 expression negatively. Our results of showed that over-expressed TLR2 induced the up-regulation of Nrf2, increased the expression of the c-Jun gene, and inhibited the expression of COX-2 in monocytes-macrophages under Pam3CSK4 stimulation.
Too many free radicles can disturb the balance between the oxidation and anti-oxidation systems of a host. GSH, SOD and CAT are important anti-oxidative substances in a host organism. Over-expression of anti-oxidants can block NF-κB activation. Also, activated NF-κB can induce secretion of pro-inflammatory factors, reduce SOD activity and increase the MDA content of a cell . Our results indicate that the consumption of anti-oxidation stress enzymes in TLR2 over-expressed cells was lower than that of the Wt cells, thereby effectively maintaining the oxidative stress balance of the cells. The result of this study also shows that TLR2 over-expression in monocytes-macrophages stimulated by Pam3CSK4 improved GSH activity and that the GSH consumed could be synthesized rapidly.
We also found that oxidative stress activated NF-κB and AP-1 leading to the production of pro-inflammatory cytokines, while excessive oxidative stress activated the Nrf2 signal pathway . Nrf2-induced HO-1 expression was found to inhibit the activation of NF-κB and the secretion of MCP-1 in endothelial cells stimulated by TNF-α . Additionally, expression of iNOS in Nrf2 knockout mice was significantly higher than that seen in Wt mice . Here, at 8 h post-stimulation with Pam3CSK4, TLR2 over-expression in transgenic goat monocytes-macrophages was found to up-regulate expression of the Nrf2 gene. Expression of the pro-inflammatory factors TNF-α decreased. The activity of iNOS was lower than that of the control. Importantly, the transgenic group experienced a weaker inflammatory reaction than that of the control group by the ear skin regional inflammation test. It indicates that TLR2 up-regulated expression of the Nrf2 gene, thereby reducing the level of oxidative injury in the host.
AP-1 family members include Jun and Fos. Jun subclasses include c-jun and JunB. Fos subclasses include c-fos and FosB. Different types of AP-1 transcription factor dimer combinations have different functions in gene expression regulation. AP-1 positively regulates the expression of most genes, but increased levels of its c-Fos and c-Jun subunits negatively regulate expression of some genes . The NQO1 gene ARE contains an AP-1 binding site. NQO1 induced by ARE is positively regulated by Nrf2 and negatively regulated by c-Fos . NQO1 and GST are highly expressed in c-Fos knockout mice , and our results are consistent with these findings. Over-expressed TLR2 up-regulated the expression of AP-1 and its c-Fos and c-Jun gene subunits; indeed, their expression trends were similar. Expression of the anti-oxidation genes NQO1, GSTα1, SOD1 and CAT were inhibited.
HO-1 is a rate-limiting enzyme in heme catalysis and the protection system comprising HO-1–bilirubin–carbon monoxide (CO) widely participate in anti-inflammatory reactions and oxidative stress injuries. Similar to NO, CO was also found to reduce the production of inflammatory factors . Researchers have discovered that activation of the HO-1 gene inhibited the activity of the AP-1 protein . However, many studies have shown that AP-1 is involved in the induction of HO-1 expression [40, 41]. In Nrf2 knockout cells, nitrite improved the activity of AP-1 through the JNK/c-Jun pathway and up-regulated HO-1 expression. Lipoteichoic acid induced expression of the HO-1 gene through the TLR2/MyD88/c-Src/NADPH oxidase pathway in tracheal smooth muscle cells . The results of our study showed that, in Pam3CSK4 stimulated goat monocytes-macrophages, the expression of AP-1 and PI3K were up-regulated in the TLR2 over-expression group; this facilitated expression of the Nrf2 gene and induced an increase in HO-1 gene expression.
Our study showed that, stimulated by the synthetic bacterial lipoprotein Pam3CSK4, TLR2 over-expressed in goat monocytes-macrophages triggered the expression of the anti-oxidation gene Nrf2, up-regulated expression of the HO-1 gene, inhibited an excessive immuno-response, and enhanced the anti-oxidative stress injury in the host through activating the AP-1 and PI3K signal pathways. TLR2 over-expressed in goats might reduce the inflammatory and oxidative stress damage.
This work was supported by grants from National Transgenic Creature Breeding Grand Project (2014ZX08008-005), Chinese Universities Scientific Fund (2014BH032), and Natural Science Foundation of China (31501953, 31471352, 31471400 and 31171380).
Availability of data and materials
All data supporting our findings are included in the manuscript.
Conceived and designed the experiments: SLD and ZXL. Performed the experiments: SLD, KY and WQJ. Analyzed the data: SLD and ZXL. Contributed reagents/materials/analysis tools: YL, STW, GSL and YCY. Wrote the paper: SLD, BLZ, ZD and YXL. All read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
The experimental protocol used in this study, including artificial insemination, intradermic injection, surgical biopsy, and blood collection, was approved by the China Agricultural University, and the whole procedure was carried out in strict accordance with the protocol approved by the Animal Welfare Committee of China Agricultural University (Permit Number: XK662).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Weese JS, Dick H, Willey BM, McGeer A, Kreiswirth BN, Innis B, et al. Suspected transmission of methicillin-resistant Staphylococcus aureus between domestic pets and humans in veterinary clinics and in the household. Vet Microbiol. 2006;115(1–3):148–55.View ArticlePubMedGoogle Scholar
- Du E, Wang W, Gan L, Li Z, Guo S, Guo Y. Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens. J Anim Sci Biotechnol. 2016;7:19.View ArticlePubMedPubMed CentralGoogle Scholar
- Goldammer T, Zerbe H, Molenaar A, Schuberth HJ, Brunner RM, Kata SR, et al. Mastitis increases mammary mRNA abundance of beta-defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle. Clin Diagn Lab Immunol. 2004;11(1):174–85.PubMedPubMed CentralGoogle Scholar
- Ogus AC, Yoldas B, Ozdemir T, Uguz A, Olcen S, Keser I, et al. The Arg753GLn polymorphism of the human toll-like receptor 2 gene in tuberculosis disease. Eur Respir J. 2004;23(2):219–23.View ArticlePubMedGoogle Scholar
- Echchannaoui H, Frei K, Schnell C, Leib SL, Zimmerli W, Landmann R. Toll-like receptor 2-deficient mice are highly susceptible to Streptococcus pneumoniae meningitis because of reduced bacterial clearing and enhanced inflammation. J Infect Dis. 2002;186(6):798–806.View ArticlePubMedGoogle Scholar
- Schmaler M, Jann NJ, Götz F, Landmann R. Staphylococcal lipoproteins and their role in bacterial survival in mice. Int J Med Microbiol. 2010;300(2–3):155–60.View ArticlePubMedGoogle Scholar
- Fournier B. The function of TLR2 during staphylococcal diseases. Front Cell Infect Microbiol. 2013;2:167.View ArticlePubMedPubMed CentralGoogle Scholar
- Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res. 2010;107(9):1058–70.View ArticlePubMedPubMed CentralGoogle Scholar
- Tkachev VO, Menshchikova EB, Zenkov NK. Mechanism of the Nrf2/Keap1/ARE signaling system. Biochemistry (Mosc). 2011;76(4):407–22.View ArticleGoogle Scholar
- Bellezza I, Mierla AL, Minelli A. Nrf2 and NF-κB and their concerted modulation in cancer pathogenesis and progression. Cancers (Basel). 2010;2(2):483–97.View ArticleGoogle Scholar
- Piantadosi CA, Withers CM, Bartz RR, MacGarvey NC, Fu P, Sweeney TE, et al. Heme oxygenase-1 couples activation of mitochondrial biogenesis to anti-inflammatory cytokine expression. J Biol Chem. 2011;286(18):16374–85.View ArticlePubMedPubMed CentralGoogle Scholar
- Paine A, Eiz-Vesper B, Blasczyk R, Immenschuh S. Signaling to heme oxygenase-1 and its anti-inflammatory therapeutic potential. Biochem Pharmacol. 2010;80(12):1895–903.View ArticlePubMedGoogle Scholar
- Frantz S, Vincent KA, Feron O, Kelly RA. Innate immunity and angiogenesis. Circ Res. 2005;96(1):15–26.View ArticlePubMedGoogle Scholar
- Deng S, Yu K, Zhang B, Yao Y, Liu Y, He H, et al. Effects of over-expression of TLR2 in transgenic goats on pathogen clearance and role of up-regulation of lysozyme secretion and infiltration of inflammatory cells. BMC Vet Res. 2012;8:196.View ArticlePubMedPubMed CentralGoogle Scholar
- Ma H, Ning ZH, Lu Y, Han HB, Wang SH, Mu JF, et al. Monocytes-macrophages phagocytosis as a potential marker for disease resistance in generation 1 of dwarf chickens. Poult Sci. 2010;89(9):2022–9.View ArticlePubMedGoogle Scholar
- Satoh M, Shimoda Y, Akatsu T, Ishikawa Y, Minami Y, Nakamura M. Elevated circulating levels of heat shock protein 70 are related to systemic inflammatory reaction through monocyte Toll signal in patients with heart failure after acute myocardial infarction. Eur J Heart Fail. 2006;8(8):810–5.View ArticlePubMedGoogle Scholar
- Song Z, Deng X, Chen W, Xu J, Chen S, Zhong H, et al. Toll-like receptor 2 agonist Pam3CSK4 up-regulates FcεRI receptor expression on monocytes from patients with severe extrinsic atopic dermatitis. J Eur Acad Dermatol Venereol. 2015;29(11):2169–76.View ArticlePubMedGoogle Scholar
- Supajatura V, Ushio H, Nakao A, Akira S, Okumura K, Ra C, et al. Differential responses of mast cell Toll-like receptors 2 and 4 in allergy and innate immunity. J Clin Invest. 2002;109(10):1351–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Mersmann J, Berkels R, Zacharowski P, Tran N, Koch A, Iekushi K, et al. Preconditioning by toll-like receptor 2 agonist Pam3CSK4 reduces CXCL1-dependent leukocyte recruitment in murine myocardial ischemia/reperfusion injury. Crit Care Med. 2010;38(3):903–9.View ArticlePubMedGoogle Scholar
- Shi Q, Le X, Abbruzzese JL, Wang B, Mujaida N, Matsushima K, et al. Cooperation between transcription factor AP-1 and NF-kappaB in the induction of interleukin-8 in human pancreatic adenocarcinoma cells by hypoxia. J Interferon Cytokine Res. 1999;19(12):1363–71.View ArticlePubMedGoogle Scholar
- Abarbanell AM, Wang Y, Herrmann JL, Weil BR, Poynter JA, Manukyan MC, et al. Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2010;298(5):H1529–36.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhong J, Shi QQ, Zhu MM, Shen J, Wang HH, Ma D, et al. MFHAS1 is associated with sepsis and stimulates TLR2/NF-κB signaling pathway following negative regulation. PLoS One. 2015;10(11), e0143662.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang J, Hu Y, Deng WW, Sun B. Negative regulation of Toll-like receptor signaling pathway. Microbes Infect. 2009;11(3):321–7.View ArticlePubMedGoogle Scholar
- Ruth MR, Field CJ. The immune modifying effects of amino acids on gut-associated lymphoid tissue. J Anim Sci Biotechnol. 2013;4(1):27.View ArticlePubMedPubMed CentralGoogle Scholar
- Blumenthal A, Kobayashi T, Pierini LM, Banaei N, Ernst JD, Miyake K, et al. RP105 facilitates macrophage activation by Mycobacterium tuberculosis lipoproteins. Cell Host Microbe. 2009;5(1):35–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Agrawal S, Agrawal A, Doughty B, Gerwitz A, Blenis J, Van Dyke T, et al. Cutting edge: different Toll-like receptor agonists instruct dendritic cells to induce distinct Th responses via differential modulation of extracellular signal-regulated kinase-mitogen-activated protein kinase and c-Fos. J Immunol. 2003;171(10):4984–9.View ArticlePubMedGoogle Scholar
- Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol. 2010;11(5):373–84.View ArticlePubMedGoogle Scholar
- Ho FM, Kang HC, Lee ST, Chao Y, Chen YC, Huang LJ, et al. The anti-inflammatory actions of LCY-2-CHO, a carbazole analogue, in vascular smooth muscle cells. Biochem Pharmacol. 2007;74(2):298–308.View ArticlePubMedGoogle Scholar
- Lee SH, Sohn DH, Jin XY, Kim SW, Choi SC, Seo GS. 2′, 4′, 6′-tris (methoxymethoxy) chalcone protects against trinitrobenzene sulfonic acid-induced colitis and blocks tumor necrosis factor-alpha-induced intestinal epithelial inflammation via heme oxygenase 1-dependent and independent pathways. Biochem Pharmacol. 2007;74(6):870–80.View ArticlePubMedGoogle Scholar
- Healy ZR, Lee NH, Gao X, Goldring MB, Talalay P, Kensler TW, et al. Divergent responses of chondrocytes and endothelial cells to shear stress: cross-talk among COX-2, the phase 2 response, and apoptosis. Proc Natl Acad Sci U S A. 2005;102(39):14010–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Yao XM, Chen H, Li Y. Protective effect of bicyclol on liver injury induced by hepatic warm ischemia/reperfusion in rats. Hepatol Res. 2009;39(8):833–42.View ArticlePubMedGoogle Scholar
- Pedersen TX, Leethanakul C, Patel V, Mitola D, Lund LR, Danø K, et al. Laser capture microdissection-based in vivo genomic profiling of wound keratinocytes identifies similarities and differences to squamous cell carcinoma. Oncogene. 2003;22(25):3964–76.View ArticlePubMedGoogle Scholar
- Malhotra D, Thimmulappa R, Navas-Acien A, Sandford A, Elliott M, Singh A, et al. Decline in NRF2-regulated antioxidants in chronic obstructive pulmonary disease lungs due to loss of its positive regulator, DJ-1. Am J Respir Crit Care Med. 2008;178(6):592–604.View ArticlePubMedPubMed CentralGoogle Scholar
- Innamorato NG, Rojo AI, García-Yagüe AJ, Yamamoto M, De Ceballos ML, Cuadrado A. The transcription factor Nrf2 is a therapeutic target against brain inflammation. J Immunol. 2008;181(1):680–9.View ArticlePubMedGoogle Scholar
- Ogawa S, Lozach J, Jepsen K, Sawka-Verhelle D, Perissi V, Sasik R, et al. A nuclear receptor corepressor transcriptional checkpoint controlling activator protein 1-dependent gene networks required for macrophage activation. Proc Natl Acad Sci U S A. 2004;101(40):14461–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Jaiswal AK. Regulation of genes encoding NAD (P) H: quinone oxidoreductases. Free Radic Biol Med. 2000;29(3–4):254–62.View ArticlePubMedGoogle Scholar
- Wilkinson J, Radjendirane V, Pfeiffer GR, Jaiswal AK, Clapper ML. Disruption of c-Fos leads to increased expression of NAD (P) H: quinone oxidoreductase1 and glutathione S-transferase. Biochem Biophys Res Commun. 1998;253(3):855–8.View ArticlePubMedGoogle Scholar
- MacGarvey NC, Suliman HB, Bartz RR, Fu P, Withers CM, Welty-Wolf KE, et al. Activation of mitochondrial biogenesis by heme oxygenase-1-mediated NF-E2-related factor-2 induction rescues mice from lethal Staphylococcus aureus sepsis. Am J Respir Crit Care Med. 2012;185(8):851–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Calkins MJ, Johnson DA, Townsend JA, Vargas MR, Dowell JA, Williamson TP, et al. The Nrf2/ARE pathway as a potential therapeutic target in neurodegenerative disease. Antioxid Redox Signal. 2009;11(3):497–508.View ArticlePubMedPubMed CentralGoogle Scholar
- Terry CM, Clikeman JA, Hoidal JR, Callahan KS. Effect of tumor necrosis factor-alpha and interleukin-1 alpha on heme oxygenase-1 expression in human endothelial cells. Am J Physiol. 1998;274(3 Pt 2):H883–91.PubMedGoogle Scholar
- Harada H, Sugimoto R, Watanabe A, Taketani S, Okada K, Warabi E, et al. Differential roles for Nrf2 and AP-1 in upregulation of HO-1 expression by arsenite in murine embryonic fibroblasts. Free Radic Res. 2008;42(4):297–304.View ArticlePubMedGoogle Scholar
- Lee IT, Wang SW, Lee CW, Chang CC, Lin CC, Luo SF, et al. Lipoteichoic acid induces HO-1 expression via the TLR2/MyD88/c-Src/NADPH oxidase pathway and Nrf2 in human tracheal smooth muscle cells. J Immunol. 2008;181(7):5098–110.View ArticlePubMedGoogle Scholar