Fig. 1

Overexpression of TLR2 in goats. a Structure of TLR2 plasmids. b TLR2-positive goats were detected by Southern blot analysis. Lanes: c, control (Wt); 1–5, genomic DNA. c TLR2 expression in monocytes-macrophages was detected by qRT-PCR. d ELISA analysis of TLR2 expression in goat sera. e ELISA analysis of TLR2 expression in monocytes-macrophages. f Expression of NF-κB and g AP-1 was detected by qRT-PCR. (h), (i), (j) and (k). The gene expression levels of c-Fos, c-Jun and PI3K were quantified by RT-PCR. Tg: Transgenic goat, Wt: wild type. The results are expressed as the means ± SEM. ^* P < 0.05 in Tg versus Wt groups