Fetal and neonatal programming of postnatal growth and feed efficiency in swine
© The Author(s). 2017
Received: 3 October 2016
Accepted: 18 April 2017
Published: 5 May 2017
Maternal undernutrition or overnutrition during pregnancy alters organ structure, impairs prenatal and neonatal growth and development, and reduces feed efficiency for lean tissue gains in pigs. These adverse effects may be carried over to the next generation or beyond. This phenomenon of the transgenerational impacts is known as fetal programming, which is mediated by stable and heritable alterations of gene expression through covalent modifications of DNA and histones without changes in DNA sequences (namely, epigenetics). The mechanisms responsible for the epigenetic regulation of protein expression and functions include chromatin remodeling; DNA methylation (occurring at the 5´-position of cytosine residues within CpG dinucleotides); and histone modifications (acetylation, methylation, phosphorylation, and ubiquitination). Like maternal malnutrition, undernutrition during the neonatal period also reduces growth performance and feed efficiency (weight gain:feed intake; also known as weight-gain efficiency) in postweaning pigs by 5–10%, thereby increasing the days necessary to reach the market body-weight. Supplementing functional amino acids (e.g., arginine and glutamine) and vitamins (e.g., folate) play a key role in activating the mammalian target of rapamycin signaling and regulating the provision of methyl donors for DNA and protein methylation. Therefore, these nutrients are beneficial for the dietary treatment of metabolic disorders in offspring with intrauterine growth restriction or neonatal malnutrition. The mechanism-based strategies hold great promise for the improvement of the efficiency of pork production and the sustainability of the global swine industry.
KeywordsEpigenetics Fetal programming Gene expression Neonatal programming Nutrition
The gestational (114 d) and neonatal (21 d after birth) periods are two critical phases in swine production . Among livestock mammals, pigs exhibit the highest rates of embryonic mortality, intrauterine growth restriction (IUGR), and neonatal deaths [2–4]. These problems are exacerbated by a variety of factors encountered in the different phases of swine production, including extreme ranges of environmental temperatures, feed hygiene and safety, suboptimal nutrition, and disease . When adverse conditions occur during gestation or nursing, the negative impacts on the offspring can last for their entire life cycle and can be carried over to the next generation or beyond . This concept is called fetal or neonatal programming , which involves the covalent modifications of nucleotide bases in DNAs without changes in their sequences [8–10]. Thus, the global swine industry must overcome enormous challenges to achieve a high efficiency of pork production and high economic returns. One approach is to optimize the nutrition of the mothers and neonates [6, 11]. The main objective of this article is to highlight and integrate the complex aspects of swine biological characteristics, IUGR, as well as the fetal and neonatal programming of postnatal growth and feed efficiency (weight gain:feed intake) in pigs.
Unique biological characteristics of swine relevant to nutrition
First, the pig has no brown adipose tissue (BAT) in its life cycle to oxidize long-chain fatty acids and glucose . Thus, there is no production of non-shivering heat in neonatal pigs, therefore contributing to the development of hypothermia and high rates of mortality in them, particularly in IUGR piglets which have a higher risk for metabolic disorders (including hypoglycemia) than normal-birth weight piglets . On the other hand, the dam gains excessive amounts of maternal subcutaneous white adipose tissue (WAT) during pregnancy when fed either ad libitum or >50% of ad libitum feed intake . While this metabolic arrangement may be beneficial for conserving maternal fat reserves in the face of limited food intake during species evolution, the adverse impacts of such a maternal adaptation mechanism include a high rate of embryonic/fetal mortality during early gestation . In addition, sows with excess WAT exhibit impaired lactation performance post-partum . Therefore, in the modern swine industry, gestating gilts are fed only a 2 to 2.2 kg diet (containing 12% CP) daily, which is only ~50% of their ad libitum feed intake, to minimize maternal WAT accretion. Clearly, this is a case of underfeeding in nutritional terms. The production-imposed reduction in the feed intake of pregnant gilts results in the inadequate provision of amino acids (AAs) in the gestation diets, thereby impairing fetal-pig growth and development . Three of four nutritionally important AAs are L-arginine, L-glutamine, glycine, and L-proline for gestating gilts . Thus, the unique characteristics of fat metabolism in swine limit their dietary intakes of energy and AAs during the entire period of pregnancy, therefore increasing the risk for IUGR and negatively impacting the vigor, survival, growth and feed efficiency of postnatal pigs.
Second, pigs have limited ketogenesis (the production of acetoacetate and β-hydroxybutyrate from fatty acids) at any developmental stage due to their low expression of hepatic 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase [16, 17]. Thus, pigs have very low concentrations of ketone bodies (<0.2 mmol/L) in plasma under either fed or food-deprived conditions. Although acetoacetate and β-hydroxybutyrate are actively utilized for ATP production by extra-hepatic tissues, such as the brain, skeletal muscle, heart, kidneys, and small intestine in mammals (including pigs) , the endogenously-generated ketone bodies only contribute a negligible amount of energy for swine. Consequently, in response to fasting or infections, when the concentration of glucose in plasma is substantially reduced, this sugar can only supply ~ 40% of the energy normally required by the brain . This leads to impairments in neurological function and animal behavior, as well as increases in the rates of morbidity and mortality in pigs, particularly during the neonatal period . When piglets die, their feed efficiency is zero.
Third, fetal pigs have a limited synthesis of fatty acids and triacylglycerols (TAG) . Thus, at birth, the piglet has only 1% lipids in its body , which is in contrast to many other mammalian newborns (e.g., humans and cattle), which possess 10% or more fats . The exceedingly small amount of endogenous lipids limits ATP production from these nutrients in neonatal pigs, leading to the use of glucose and AAs as the major metabolic fuels. This, along with the near absence of ketogenesis, readily results in hypoglycemia and the reduced availability of AAs for protein synthesis, particularly when milk intake is insufficient . Additionally, because subcutaneous fats can be used for insulation, the limited amount of fats in newborn pigs further increases the risk for hypothermia and, therefore, mortality in them, especially in a cold environment . Deaths of preweaning piglets will result in no feed value for sows. The ability to synthesize TAG (occurring primarily in WAT) rapidly develops in well-fed piglets after birth, which results in a body-lipid content of 14% at 2 weeks of age . Compared with pigs with a normal birth weight, surviving IUGR pigs have a greater capacity for TAG deposition in the body during the growing-finishing period and, therefore, exhibit a lower feed efficiency and lower quality pork .
Fourth, pigs possess no functional sweat glands and, therefore, must use the inadequate mechanism of panting to dissipate heat . Thus, growing-finishing, gestating or lactating swine are highly susceptible to heat stress, as well as the associated oxidative stress, resulting in an excessive production of reactive oxygen species [26, 27]. Under experimental conditions, increasing environmental temperatures from 23 °C to 33 °C markedly reduces feed intake, growth performance, and the efficiency of nutrient utilization for weight gains in swine [28–30], and, at the same time, impairs immune responses  and muscle protein synthesis . Interestingly, the pig’s breed does not influence physiological responses to thermal stress [33, 34]. Thus, pigs raised in naturally warm areas exhibit poor production performance, including low daily weight gain , low protein deposition in muscle , poor lactation, and low litter size . To date, a climate change towards global warming is expected to negatively impact swine production. Mader et al.  estimated that, with CO2 doubling and tripling, the time necessary for raising pigs from 50 to 110 kg in warm seasons would be increased by up to 30% and 74%, respectively, in the southernmost regions of the U.S. The lack of sweat glands in swine presents a challenge to design nutritional means for improving feed efficiency in growing pigs, particularly in IUGR pigs raised at high ambient temperatures.
Intrauterine Growth Restriction (IUGR) in swine
IUGR is defined as impaired growth and development of the mammalian embryo/fetus or its organs during pregnancy . In animal studies, IUGR is identified as fetal or birth weight less than two standard deviations of the mean body weight for gestational age. For crossbred sows (Yorkshire × Landrace dams and Duroc × Hampshire boars), the mean birth weight is 1.4 kg, and a piglet with a birth weight of less than 1.1 kg can be considered to have IUGR. Despite advances in nutrition and management techniques, IUGR remains a significant problem in swine production [3, 4]. Before d 35 of gestation, porcine embryos are uniformly distributed within each uterine horn. After this time in gestation, uterine capacity becomes a limiting factor for fetal growth even though the fetuses are distributed relatively uniformly . Additionally, the proportion of low birth weight pigs at farrowing has increased in recent years due to the successful genetic selection for increased litter size, which results in increased uterine crowding and the associated decrease in placental weight per fetus [3, 39]. Approximately 24% of newborn piglets from gilts fed a 12%-CP diet have a birth weight of < 1.1 kg . In some litters, most or nearly all of the piglets have reduced birth weights (<1.1 kg), particularly when a part or majority of the pregnancy period is subjected to environmental stress (e.g., hot or cold temperatures or infections) . At birth, runt piglets may weigh only one-half or even one-third as much as their largest littermates, and key organs involved in nutrient digestion and utilization in runt pigs are disproportionately smaller than those of the larger littermates . Thus, a major goal of feeding is to enhance piglet birth weight and reduce its variation within litters .
Most IUGR piglets die before weaning, and IUGR piglets (<1.10-kg birth weights) account for 76% of preweaning deaths in pigs . IUGR has permanent negative impacts on organ structure, neonatal adjustment, preweaning survival, postnatal growth, feed efficiency, lifetime health, skeletal-muscle composition, excessive accumulation of WAT, meat quality, reproductive performance, and the onset of adult diseases [14, 42]. Altered organ mass and structure, such as reduced numbers of pancreatic islets, reduced numbers of kidney glomeruli, or reduced numbers of muscle fibers, is one of the consequences of fetal programming and is equally important to functional consequences. At present, farms will cull all IUGR piglets, and there is no nutritional support to increase their growth or survival during the suckling and postweaning periods . Thus, although IUGR may be considered to be a natural mechanism to protect the dam in case of maternal undernutrition, it has adverse effects on the survival and growth performance of the progeny and the efficiency of pig production .
Factors contributing to IUGR in swine
Maternal undernutrition and IUGR
Undernutrition before breeding or during the periconceptual period
Much evidence shows that maternal malnutrition immediately before breeding or during the periconceptual period negatively affects oocyte quality, embryonic development and survival, and in fact ‘programs’ the timing of delivery of the newborn . Insufficient feed intake remains a significant problem for lactating sows before breeding, because the mobilization of nutrient reserves for milk production results in a severe catabolic state and a prolonged interval from farrowing to estrus [39, 43]. Feed consumption of sows during lactation can be as low as 70% of the NRC requirements . Inadequate nutrition increases the losses of body weight (BW) and backfat in lactating sows and also prolongs weaning-to-estrus intervals . When sows enter pregnancy, the suboptimal nutritional status (namely premating maternal undernutrition), coupled with restricted feed intake, impairs the growth and development of early embryos and fetuses [2, 13, 15]. For example, in primiparous sows, restricting feed intake by 50% during lactation (a reduction from 5.0 to 2.5 kg/d between d 14 and d 21 of lactation) before mating reduces the weight of both male and female fetuses, as well as the survival of female embryos at d 30 of gestation . Furthermore, reducing the intake of a complete ration by 50% for 2 estrous cycles before mating decreases fetal weight at d 30 of pregnancy in gilts . Likewise, maternal undernutrition during the periconceptual period or the entire gestation reduces the fetal growth of pigs . When the dietary provision of water, vitamins and minerals is sufficient, it is energy and protein/AA intake that are the major nutritional factors affecting fetal growth.
Undernutrition during late gestation
Complete fasting of pregnant gilts for 10 d between d 100 and d 114 of gestation does not influence the body fat of fetuses due to their naturally limited ability for prenatal fat synthesis . Of interest, fasting during the last 20 d of gestation increases the fat content of fetal adipose tissue , possibly due to the increased transfer of fatty acids from the mother to her fetuses. Decreasing feed intake by 28% after d 80 of gestation (2.5 vs. 1.8 kg feed/d) reduces fetal growth in gilts . Because most fetal growth occurs in the last 3 weeks of pregnancy, undernutrition during late gestation has a greater negative effect on the birth weight of pigs than during early- or mid- gestation .
Insulin resistance during late gestation
Maternal insulin resistance gradually develops in most mammals (including sows) late in their pregnancy . This is likely due to the inability of the liver and skeletal muscle to oxidize the fatty acids released from WAT in response to a negative energy balance. An increase in plasma and tissue levels of free fatty acids is a major factor contributing to the occurrence of insulin resistance by reducing the synthesis of nitric oxide (NO) from arginine . The low glucose tolerance of pregnant sows is associated with a high postnatal mortality of piglets and reduced efficiency of pork production . Although insulin resistance in the dam may have the potential to increase the availability of glucose and AAs for the fetus, the transfer of nutrients from mother to fetus due to reduced placental blood flow is impaired under this metabolic condition. Because insulin stimulates muscle protein synthesis and inhibits muscle protein degradation, insulin resistance increases the net rate of whole-body proteolysis and thus plasma levels of methylarginines (inhibitors of endothelial NO synthesis). As NO is the major regulator of uteroplacental blood flow, severe insulin resistance decreases the placental delivery of nutrients and oxygen to the fetus during late gestation .
Undernutrition during the entire gestation
The birth weight of piglets decreases in response to restricted feed intake during the entire gestation period (e.g., 0.9 vs. 1.9 kg/d) . In addition, compared with adequate protein feeding, birth weights as well as brain and liver weights are reduced and bone development is impaired, in the progeny of gilts fed a protein-deficient diet throughout gestation [44, 49]. Likewise, severe maternal protein undernutrition during the entire gestation impairs the development of fetal skeletal-muscle fibers in pigs . Besides the impairment of fetal growth (including bone growth), maternal undernutrition during the entire gestation will greatly reduce the longevity of the sows . This has important implications for the sustainability of the swine industry for the following reasons. First, the production efficiency of a sow is determined by the number of piglets born alive and weaned per litter within a year during her breeding lifetime . Second, litter size and piglet birth weights increase until the fourth or fifth parities, and the number of pigs weaned per sow within a year increases until the sixth and seventh parities [3, 39]. Third, healthy replacement gilts with the satisfactory nutrition status will most likely reach their fourth parity, when they are most productive for the swine operation .
In contrast to ruminants and horses, the pig generally has a remarkably high capacity to mobilize maternal energy reserves to support placental and fetal development during prolonged inanition in the presence of adequate progesterone and estrogen . Thus, a modest reduction in the dietary intake of energy alone is not sufficient to cause IUGR in pigs. For example, in low-prolific gilts fed adequate amounts of protein, vitamins and minerals, restriction of dietary energy intake (50% of controls; namely 1.82 vs. 0.91 kg diet/d) does not affect the birth weight of piglets . This likely results from the mobilization of TAG from the maternal WAT to spare AAs and glucose for fetal utilization. However, a more severe reduction in energy intake by gilts during the entire gestation from 8.0 to 2.2 Mcal of DE/d reduces birth weight, the number of skeletal-muscle fibers, muscle weight, liver weight, liver glycogen content, and serum protein concentrations in newborn piglets .
Maternal overnutrition and IUGR
Overnutrition before breeding or during the periconceptual period
Concentrations of amino acids (AAs) and metabolites in maternal plasma and reproductive performance of gestating gilts fed diets supplemented with 10–16% crude protein (CP)1
Concentrations of AAs and metabolites in maternal plasma
Cystine + cysteine, μmol/L
Reproductive performance of gilts
Total piglets born per litter, n
Total piglets born alive per litter, n
Average birth weight of all piglets born, kg
Average birth weight of all piglets born alive, kg
Total litter weight at birth for all piglets born, kg
Total litter weight at birth for all live piglets, kg
Piglets born dead per litter, n
Variations in birth weights among all piglets born,2%
Variation in birth weights among all piglets born alive,2%
Survival and growth of live-born piglets before weaning
Milk intake of sow-reared piglets,3 mL/kg BW per day
Total piglets weaned per litter, n
Total litter weight at weaning (21 days of age), kg
Negative impacts of maternal overnutrition during gestation on lactation and fetal growth
Increased feed intake by sows during all or part of gestation has a negative effect on feed intake during lactation . In multiparous sows, increasing dietary intakes of both protein and energy by 43% during the first 50 days of gestation, relative to a standard gestational diet (10.7 MJ of DE/kg and 12.0% CP), decreases the birth weights of the 2 lightest and 2 heaviest piglets in litters . Although overfeeding both energy and protein between d 25 and d 50 of gestation has no beneficial effect on muscle fiber number in the offspring, this nutritional treatment reduces the skeletal-muscle weight of newborn piglets due to a smaller fiber size . However, the high intake of dietary protein during the whole pregnancy period decreases the number of muscle fibers at birth in pigs . Furthermore, overfeeding gilts by 40% of the NRC requirements  during the entire gestation impairs fetal development and postnatal survival . Thus, overfeeding during all or part of the gestational stage has a detrimental effect on pregnancy outcomes in swine and must be avoided in feeding practices.
Impacts of IUGR on pig production
Effects of IUGR on growth performance and feed efficiency in postnatal offspring
Stages of the fetal and postnatal development of porcine skeletal muscle
Days of gestation
From conception to 25 d of gestation
Embryonic myogenesis from a common mesenchymal precursor
From 25 to 50 d of gestation
Formation of primary muscle fibers (rapid fusion of primary myoblasts)
From 50 to 90 d of gestation
Formation of secondary muscle fibers (formed on the surface of primary fibers)
From 90 to 95 d of gestation
Establishment of muscle fiber numbers
d 114 of gestation
Total numbers of muscle fibers are fixed at birth
Growth of skeletal muscle by increasing the size of its fibers (hypertrophy)a; and maturation of skeletal muscle
As noted previously [42, 67], the total number of skeletal muscle fibers at birth is lower in IUGR newborn pigs than in their littermates with a normal birth weight. This limits the extent of compensatory growth in these offspring . Thus, there are differences in prenatal and postnatal growth rates between IUGR piglets and normal litter mates, which correlate with a lower ratio of secondary to primary muscle fibers and a smaller size of the fibers in IUGR pigs . Reduced protein deposition in skeletal muscle and increased fat accretion in IUGR fetuses or offspring result from the abnormal metabolic regulation of intracellular protein turnover, adipogenesis, and mitochondrial biogenesis . Consistent with this notion, the results of proteomic analysis have shown that newborn IUGR piglets have a greater abundance of proteasome (a complex of proteolytic enzymes for nonlysosomal protein degradation) in skeletal muscle and liver, but less eukaryotic translation initiation factor-3 (a key requirement for protein synthesis) in skeletal muscle, compared to piglets with a normal birth weight . Consequently, when compared to piglets with a normal birth-weight, IUGR piglets exhibit 5–10% lower rates of daily weight gain and 5–10% lower feed efficiency (weight gain:feed intake) during the preweaning period [68–71].
Impacts of IUGR on growth and feed efficiency decrease with increasing age in pigs
Total variance in ADG accounted for by BBW
Difference in ADG between IUGR pigs (1-kg BBW) and and large-birth-weight pigs (2-kg BBW)
Daily feed intake
Difference in gain: feed ratio between IUGR pigs (1-kg BBW) and large-birth-weight pigs (2-kg BBW)
IUGR pigs with 1-kg BBW
Large-birth weight pigs with 2-kg BBW
Without any effective nutritional interventions, IUGR piglets will not be able to achieve catch-up growth at an absolute rate comparable to that of age-matched piglets with a normal birth weight either before or after weaning [69, 74]. For example, a difference of 0.31 kg in birth weight (1.04 vs. 1.35 kg) or of 0.8 kg in body weight at weaning (21 d of age) translates into an average difference of 4.4 days (159.3 vs. 154.9 d) from birth to the same market weight of 120 kg . When muscle protein synthesis is reduced, dietary energy can be partitioned toward fat deposition within skeletal muscle and the WAT. In support of this view, the fat content of market-weight carcasses from runt pigs is increased, compared with their large littermates . However, the carcass composition or the final eating quality of the pork may not differ substantially among pigs with a birth weight of 0.8 and 2.5 kg when they are fed adequately and slaughtered at the same 120-kg market weight .
IUGR and reproduction performance of offspring
Smaller female pigs have a smaller uterus and less uterine secretion than larger female pigs [38, 75]. Insufficient uterine capacity in size and function limits placental attachment and growth within the uterus, thereby reducing nutrient and gas exchange between the mother and her fetuses . In addition, when compared to female pigs with a normal birth weight, those with IUGR exhibit a delay or failure to express estrus, conceive or farrow, as well as reduced preweaning and postweaning growth performance in affected offspring . Such adverse effects of IUGR can be carried for up to three generations . There is also evidence that IUGR delays fetal follicular development of the ovaries, as well as the onset of puberty in postnatal life, in comparison to pigs with a normal birth weight . Furthermore, stressing pregnant sows daily between weeks 12 and 16 of gestation (5 min of restraint daily) delays the first estrus of the female offspring, compared to the offspring of the control, non-stressed sows (172 vs. 158 d of age) . Similarly, in gilts, the age at puberty was negatively correlated with birth weight ranging from 1.13 to 1.98 kg . Results of published studies have also indicated that the timing of under-nutrition during gestation has important effects on the development of the fetal reproductive system (e.g., hypothalamus, pituitary, and gonads) .
There is indirect evidence that neonatal nutrition affects subsequent reproductive function in pigs . For example, gilts which were raised in litters of six pigs (6 piglets/litter) prior to weaning had more corpora lutea (an indication of ovulation rate) and more embryos at d 25 of gestation than gilts which were raised in litters of 12 piglets (12 piglets/litter) . Likewise, boars raised in litters of six or less piglets (≤6 piglets/litter) reached puberty sooner and produced more sperm per ejaculate, compared with boars raised in litters of nine or more piglets (≥9 piglets/litter) . Furthermore, Estienne and Harper  reported that adult boars with a birth weight of < 1.36 kg had lower sperm concentrations and less total sperm per ejaculate than adult boars with a birth weight of > 1.86 kg, suggesting that birth weight is also a determinant of reproductive potential in males. Collectively, impaired fetal or preweaning growth may result in suboptimal reproductive performance in both female and male swine.
Fetal and neonatal programming of growth, development and feed efficiency
Epigenetics as a mechanism of fetal programming
Methylation is a key biochemical reaction affecting epigenetics, with the major donor of the methyl group being S-adenosylmethionine (a metabolite of methionine) . Other AAs (histidine, glycine, and serine) and folate also participate in one-carbon metabolism to affect the provision of S-adenosylmethionine in cells . The methyl group is donated from S-adenosylmethionine to the 5´-position of a cytosine nucleotide linked to a guanine nucleotide (CpG dinucleotide) by a phosphodiester bond. Regions with a high CpG dinucleotide content form CpG islands, which are strategically located in the regulatory regions of many genes, such as promoters and enhancers . Consequently, changes in methylation status can either facilitate (hypomethylation for most genes) or inhibit (hypermethylation for most genes) the expression of genes. Epigenetic modifications are erased and re-established in a tissue-specific manner during embryonic development . The haploid genomes of the sperm and oocyte possess different patterns of DNA methylation in a sex-specific manner . After fertilization, the paternal genome is rapidly demethylated prior to the first cell division of the zygote. In contrast, the maternal genome is protected from this demethylation event, and, instead, is gradually demethylated during the development of the blastocyst. At the blastocyst stage, most methyl marks are removed, remaining present at the elements that regulate genomic imprinting and at retroviral elements . After implantation, tissue-specific patterns of de novo DNA methylation occur. The processes of demethylation and remethylation during the development of germ cells are regulated by the phosphatidylinositol 3-kinase pathway .
Thus, through the methylation of genes, epigenetic information is heritable between cell generations . Epigenetic transgenerational inheritance can be defined as germline-mediated inheritance of epigenetic information between generations that results in phenotypic variation . In somatic cells, the methyltransferase, DNMT1 recognizes and methylates CpG dinucleotides present on the newly synthesized strand. As DNA synthesis occurs, the parental strand remains methylated, whereas the newly synthesized daughter strand does not. DNMT1 “reads” the parental strand and methylates CpG dinucleotides on the daughter strand that are complementary to methylated CpG dinucleotides on the parental DNA strand . The critical role of DNA methylation in conceptus survival is made evident by the finding that all homozygous DNMT1 knockout mice die at the morula stage of embryonic development . Epigenetic regulation of gene expression is the major mechanism responsible for the transgenerational effects of maternal nutrition on offspring .
Genetic regulation of protein expression and cell signaling
Nutrition can regulate gene expression, micro-RNA biogenesis, and epigenetics in animal cells [6, 85]. For example, dietary glutamine reduces the intestinal expression of genes that promote oxidative stress and immune activation, while increasing the intestinal expression of genes that enhance cell growth and the removal of oxidants . Consistent with its anti-oxidative and anti-fat deposition effects, dietary arginine inhibits the expression of key genes responsible for fatty acid synthesis, but stimulates the expression of key genes that are essential to fatty acid oxidation and glutathione synthesis in the WAT of rats [93, 94]. Dietary arginine also increases the expression of miRNA-15b/16 and miRNA-221/222 in the porcine umbilical vein to regulate angiogenesis and vascular remodeling . Furthermore, glycine stimulates the intestinal expression of glycine transporter 1, while reducing the activation of the mitogen-activated protein kinase signaling pathway to confer anti-inflammatory effects .
Many AAs affect cell signaling via kinases [e.g., mammalian target of rapamycin (mTOR), AMP-activated protein kinase, cGMP-dependent kinase, cAMP-dependent kinase, and mitogen-activated protein kinase)], G protein-coupled receptors, and gaseous molecules (e.g., NO, CO and H2S) to regulate nutrient metabolism . For example, dietary arginine enhances the abundance of the phosphorylated mTOR, eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1), and ribosomal protein S6 kinase 1 (S6K1), as well as the formation of the active eIF4E-eIF4G complex, but reduces the abundance of the inactive 4E-BP1-eIF4E complex in skeletal muscle . This leads to increased protein synthesis and whole-body growth . Likewise, arginine or its metabolite putrescine activates mTOR to promote protein synthesis in placental cells and their proliferation . In addition, dietary glutamine enhances intestinal integrity, cell survival, and villus height through the activation of mTOR cell signaling, while attenuating weaning-induced reduction in the abundances of occludin, claudin-1, zonula occluden (ZO)-2, and ZO-3 proteins . Furthermore, dietary glutamate regulates the expression of glutamate receptors and taste receptor signaling in the pig’s gastrointestinal tract to maintain gut motility and function . Likewise, AAs (e.g., arginine, cysteine and glycine) regulate the synthesis of NO, CO, and H2S, which participate in gaseous signaling in cells through cGMP and cAMP production to enhance blood flow, nutrient transport, anti-oxidative reactions, and immunity .
Because nutrients play a key role in gene expression, maternal or neonatal undernutrition can affect metabolic pathways and, therefore, cell growth, differentiation, and development . This notion is supported by several lines of evidence. First, maternal consumption of a low-protein diet during pregnancy increases the expression and activity of the glucocorticoid receptor in the fetal liver, which may further enhance the capacity for gluconeogenesis in adults . Of interest, maternal protein deficiency regulates mtDNA transcription in a sex-dependent manner , while increasing the hepatic expression of glucose-6-phosphatase in male piglets via histone methylation, acetylation and trimethylation, as well as micro-RNA biogenesis . Second, maternal protein restriction alters the hepatic lipid content in male offspring , as well as a high incidence of fatty liver . The latter may result, in part, from the decreased expression of peroxisomal proliferator-activated receptor-γ (PPARγ) through a micro-RNA-130b-dependent mechanism  and the increased expression of peroxisomal proliferator-activated receptor-α (PPARα)  in a tissue-specific manner. Third, maternal protein deficiency during gestation and lactation alters hepatic cholesterol metabolism in weanling piglets via the epigenetic regulation of expression of HMG-CoA reductase and cholesterol-7alpha-hydroxylase (CYP7α1) . Furthermore, a deficiency of AAs affects the phosphorylation of eEF2 (eukaryotic elongation factor 2) kinase and eIF2α (eukaryotic translation initiation factor 2 alpha) through the general control nonderepressible 2 (GCN2) protein pathway, thereby inhibiting translation initiation for polypeptide formation [111, 112]. Thus, interactions betweern nutrients and gene expression is another basis for the adverse effects of malnutriton on animal growth, development, and health.
Nutritional Interventions to Improve Embryonic, Fetal and Postnatal Survival and Growth
At first glance, augmenting dietary protein intake would seem to be a simple way to enhance swine fetal and neonatal growth under IUGR conditions. However, this approach has been reported to be detrimental to both fetal and neonatal survival. For example, when IUGR piglets were artificially fed a high-protein formula (50% more protein in comparison to sow’s milk) between 2 and 28 d of age, they exhibited poor growth and one-third of them died in association with hyperammonemia and elevated blood urea concentrations . Thus, mechanism-based means should be developed to prevent and treat IUGR and its associated metabolic disorders.
Embryonic, fetal and postnatal survival and growth
Because of ammonia toxicity, pregnant swine should not be fed a high-protein diet (e.g., ≥ 14% CP for gilts). There are reports that the prevention of maternal protein deficiency in mid-gestation sows (55–90 d) can increase the number of secondary fibers in their progeny  and, therefore, the rate of their growth and feed efficiency in the growing-finishing stage . Thus, nutritional interventions critically depend on the gestation stage.
As a functional AA, dietary supplementation with arginine is an effective means by which to improve pregnancy outcome in pigs . First, dietary supplementation with 1.0% arginine-HCl between d 30 and 114 of gestation increases the number of live-born piglets by 2 and the litter birth weight by 24% . The arginine-to-lysine ratio in the supplemental diet was 2.64, which did not affect the intestinal absorption of lysine or histidine. An arginine-to-lysine ratio of greater than 3:1 in the diet will result in antagonism among basic AAs and, therefore, should be avoided in dietary formulation . Second, dietary supplementation with 1% arginine to gilts or sows between d 14 and d 28 of gestation increases the number of live-born piglets by approximately 1 at birth , whereas supplementation with 1% arginine to highly prolific gilts and sows between d 14 and d 28 of gestation enhances the number of fetuses on d 70 by 3 per litter . Third, dietary supplementation with 0.4 or 0.8% arginine to gilts between d 14 and d 25 of gestation increases the number of live fetuses by 2 per litter . The underlying mechanisms for the beneficial effects of arginine may involve: (a) the improved development or function of corpora lutea; (b) the maintenance of adequate production of progesterone, a major hormone for maintaining pregnancy; (c) enhanced placental NO and polyamine synthesis to promote placental angiogenesis and growth, and, therefore, the transfer of nutrients from the mother to her fetuses; and (d) improvements in the cellular redox state and anti-oxidative cell signaling [114, 119].
Glutamine is one of the most abundant AAs in fetal tissues and a major energy substrate for the fetus . Because of a high rate of glutamine utilization by the rapidly growing fetus, the concentration of glutamine in the maternal plasma is reduced by 45% during late gestation in gilts, as compared to that during early gestation . Inadequate provision of glutamine to the conceptus is likely a major factor contributing to IUGR in pigs. In support of this notion, we have found that supplementing 1% glutamine to the diet of gilts between d 90 and d 114 of gestation increases the birth weight and litter birth weight of live-born piglets, when compared with the isonitrogenous control . Glutamine supplementation beneficially reduces the number of IUGR piglets, variations in birth weight, and preweaning mortality of live-born piglets by 39, 33, and 46%, respectively . Thus, glutamine is an effective nutrient to enhance the productivity and performance of gestating pigs.
Arginine plus glutamine
Arginine also cooperates with glutamine to further improve the reproductive performance of pigs. The rationale for supplementation with arginine plus glutamine is that both AAs regulate protein synthesis by activating (a) the production of polyamines, which are essential for gene expression and mRNA translation, and (b) mTOR cells signaling. We have shown that adding 0.6% glutamine and 0.4% arginine to a corn- and soybean meal-based diet reduces (a) concentrations of ammonia (–29%) and urea (–27%) in maternal plasma (indicators of improved efficiency in the utilization of dietary AAs); (b) variation in birth weights among either all piglets born (–27%) or live-born piglets (–24%); and (c) the proportion of piglets with birth weights of 0.6 to 1.29 kg (–23% for all piglets born and –22% for live-born piglets) . Furthermore, dietary supplementation with arginine plus glutamine increases (a) the number of live-born piglets by 1.4 per litter; (b) litter birth weight for either all piglets born (+10%) or live-born piglets (+15%), and (c) the proportion of piglets with birth weights of 1.3 to 1.49 kg (+37% for all piglets born and +30% for live-born piglets) (Wu et al. ).
The effects of arginine supplementation treatment in enhancing the porcine litter size and survival of porcine embryos/fetuses is expected to result in a tremendous economic return to swine producers. Specifically, an increase in the number of live-born pigs will markedly reduce production costs associated with reproduction and lactation in dams. Additionally, a reduction in the number of IUGR piglets will greatly improve the management of neonatal pigs and maximize preweaning survival and growth. Our findings provide a much needed basis for the dietary requirements of arginine and glutamine by gestating swine.
As noted previously, folate is essential for one-carbon metabolism, which is vital for the methylation of DNA and protein . Thus, vitamins play an important role in embryonic survival and growth, as well as fetal and neonatal programming . Of note, Randy Jirtle and his colleagues  discovered that maternal supplementation with folate can counteract bisphenol A-induced DNA hypomethylation and improve pregnancy outcomes in mice. Likewise, Waterland and Jirtle  demonstrated that adding a mixture of extra folate, betaine, choline, and vitamin B12 to the maternal diet of agouti mice affected the phenotype of their offspring via increasing CpG methylation at the A(vy) locus . Consistent with the findings from the rodent studies, Liu et al.  have reported the differential expression of the proteins related to the metabolism of nutrients and oxidative stress in the livers of IUGR fetal pigs which have low concentrations of folate . Interestingly, maternal supplementation with folic acid can alter the expression of proteins which are critical for immune and oxidative responses, as well as hepatic energy metabolism in newborn piglets . The underling mechanisms likely involve the improvement of anti-oxidative signaling in hepatocytes and possibly other cell types.
Postnatal survival and growth
Enterocytes are responsible for the endogenous synthesis of arginine in pigs  and, therefore, are critical for maximal growth of piglets and ammonia detoxification via the liver . However, the development of the small intestine is compromised in IUGR pigs . Thus, improving the integrity and function of the gut is an effective means to ameliorate neonatal death and growth restriction in IUGR offspring. For example, oral administration of glutamine (0.5 g/kg of BW twice daily; 1 g/kg of BW per day) between d 0 and d 21 of age enhances the growth of IUGR piglets by 16% while reducing their preweaning mortality by 48% . Additionally, concentrations of ammonia in the plasma are 19% lower in the glutamine-supplemented IUGR piglets than in the control group , indicating that glutamine stimulates whole-body protein synthesis and inhibits whole-body AA oxidation. These findings have important implications for the nutritional management of compromised young pigs , improvement in the utilization of dietary protein for lean tissue growth , and the sustainability of global pork production . While leucine supplementation can enhance intestinal development and growth, as well as whole-body growth in piglets with a normal birth weight , this method has a detrimental effect in IUGR piglets for yet unknown reasons . Thus, the mechanisms responsible for the high rate of mortality in IUGR pigs should be further elucidated so that effective means will be developed to save their lives and enhance their productivity.
In summary, a large body of evidence shows that maternal under- or over-nutrition and other environmental stresses immediately before breeding or during pregnancy (particularly early gestation) negatively influence the metabolism, growth and development of the porcine fetus, as well as numerous metabolic pathways, feed efficiency, and the disease susceptibility of the affected offspring. The underlying mechanisms involve the epigenetic regulation of gene expression, key transcription factors, protein abundance and activity, and multiple signaling pathways. Nutritional interventions involving dietary supplementation with functional AAs (e.g., arginine and glutamine) or possibly certain vitamins (e.g., folate) related to one-carbon metabolism and the provision of methyl groups can help overcome the adverse effects of maternal fetal and neonatal programming on the growth performance, feed efficiency, and well-being of IUGR offspring. Optimal AA nutrition has great promise to improve fetal growth, development, and survival not only through cell signaling but also via the epigenetic regulation of protein expression and functions.
We thank our colleagues for collaboration on nutrition and reproduction research.
This research was supported by the National Basic Research Program of China (2013CB127302), the National Natural Science Foundation of China (31272450 and 31572412), Competitive Grants from the Animal Reproduction Program (no. 2014-67015-21770) and Animal Growth & Nutrient Utilization Programs (no. 2015-67015-23276) of the USDA National Institute of Food and Agriculture, and Texas A&M AgriLife Research (H-8200).
Availability of data and materials
GW conceived this project. YI, ZLW, ZLD, and GW wrote the manuscript. XLW, JL, and BGW contributed to the discussion and revision of the article. GW had the primary responsibility for the content of the paper. All authors read and approved this manuscript.
The authors declare that they have no competing interests.
Consent for publication
All authors read and approved the final manuscript.
Animal work in our laboratories which was reviewed in this article was approved by China Agricultural University and Texas A&M University Animal Care and Use Committee.
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- Wu G, Bazer FW, Dai ZL, Li DF, Wang JJ, Wu ZL. Amino acid nutrition in animals: protein synthesis and beyond. Annu Rev Anim Biosci. 2014;2:387–417.PubMedView ArticleGoogle Scholar
- Foxcroft GR, Dixon WT, Novak S, Putman CT, Town SC, Vinsky MDA. The biological basis for prenatal programming of postnatal performance in pigs. J Anim Sci. 2006;84(E. Suppl):E105–12.PubMedView ArticleGoogle Scholar
- Freking BA, Lents CA, Vallet JL. Selection for uterine capacity improves lifetime productivity of sows. Anim Reprod Sci. 2016;167:16–21.PubMedView ArticleGoogle Scholar
- Bazer FW, Spencer TE, Johnson GA, Burghardt RC, Wu G. Comparative aspects of implantation. Reproduction. 2009;138:195–209.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Burghardt RC, Johnson GA, Kim SW, Li XL, et al. Impacts of amino acid nutrition on pregnancy outcome in pigs: mechanisms and implications for swine production. J Anim Sci. 2010;88:E195–204.PubMedView ArticleGoogle Scholar
- Ji Y, Wu ZL, Dai ZL, Sun KJ, Wang JJ, Wu G. Nutritional epigenetics with a focus on amino acids: Implications for the development and treatment of metabolic syndrome. J Nutr Biochem. 2016;27:1–8.PubMedView ArticleGoogle Scholar
- Barker DJP, Clark PM. Fetal undernutrition and disease in later life. Rev Reprod. 1997;2:105–12.PubMedView ArticleGoogle Scholar
- Bertram C, Trowern AR, Copin N, Jackson AA, Whorwood CB. The maternal diet during pregnancy programs altered expression of the glucocorticoid receptor and type 2 11beta-hydroxysteroid dehydrogenase: potential molecular mechanisms underlying the programming of hypertension in utero. Endocrinology. 2001;142:2841–53.View ArticleGoogle Scholar
- Reynolds LP, Caton JS. Role of the pre- and post-natal environment in developmental programming of health and productivity. Mol Cell Endocrinol. 2012;354:54–9.PubMedView ArticleGoogle Scholar
- Wu G, Imhoff-Kunsch B, Girard AW. Biological mechanisms for nutritional regulation of maternal health and fetal development. Paediatr Perinatal Epidemiol. 2012;26 Suppl 1:4–26.View ArticleGoogle Scholar
- Belkacemi L, Nelson DM, Desai M, Ross MG. Maternal undernutrition influences placental-fetal development. Biol Reprod. 2010;83:325–31.PubMedView ArticleGoogle Scholar
- Satterfield MC, Wu G. Growth and development of brown adipose tissue: significance and nutritional regulation. Front Biosci. 2011;16:1589–608.View ArticleGoogle Scholar
- Kim SW, Hurley WL, Wu G, Ji F. Ideal amino acid balance for sows during gestation and lactation. J Anim Sci. 2009;87:E123–32.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Wallace JM, Spencer TE. BOARD-INVITED REVIEW: Intrauterine growth retardation: Implications for the animal sciences. J Anim Sci. 2006;84:2316–37.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Johnson GA, Burghardt RC, Li XL, Dai ZL, et al. Maternal and fetal amino acid metabolism in gestating sows. Soc Reprod Fertil Suppl. 2013;68:185–98.Google Scholar
- Duée PH, Pégorier JP, Quant PA, Herbin C, Kohl C, Girard J. Hepatic ketogenesis in newborn pigs is limited by low mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase activity. Biochem J. 1994;298:207–12.PubMedPubMed CentralView ArticleGoogle Scholar
- Odle J, Lin X, van Kempen TA, Drackley JK, Adams SH. Carnitine palmitoyltransferase modulation of hepatic fatty acid metabolism and radio-HPLC evidence for low ketogenesis in neonatal pigs. J Nutr. 1995;125:2541–9.PubMedGoogle Scholar
- Jobgen WS, Fried SK, Fu WJ, Meininger CJ, Wu G. Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates. J Nutr Biochem. 2006;17:571–88.PubMedView ArticleGoogle Scholar
- Wu G, Marliss EB. Interorgan metabolic coordination during fasting and underfeeding: An adaptation for mobilizing fat and sparing protein in man. In: Anderson GH, Kennedy SH, editors. Biology of Feast and Famine. San Diego: Academic; 1992. p. 219–44.Google Scholar
- Hausman GJ, Kasser TR, Martin RJ. The effect of maternal diabetes and fasting on fetal adipose tissue histochemistry in the pig. J Anim Sci. 1982;55:1343–50.PubMedView ArticleGoogle Scholar
- Wu G, Ott TL, Knabe DA, Bazer FW. Amino acid composition of the fetal pig. J Nutr. 1999;129:1031–8.PubMedGoogle Scholar
- Widdowson EM. Milk and the newborn animal. Proc Nutr Soc. 1984;43:87–100.PubMedView ArticleGoogle Scholar
- Rezaei R, Wang WW, Wu ZL, Dai ZL, Wang JJ, Wu G. Biochemical and physiological bases for utilization of dietary amino acids by young pigs. J Anim Sci Biotechnol. 2013;4:7.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu G, Knabe DA, Kim SW. Arginine nutrition in neonatal pigs. J Nutr. 2004;134:2783S–90S.PubMedGoogle Scholar
- Huynh TTT, Aarnink AJA, Truong CT, Kemp B, Verstegen MWA. Effects of tropical climate and water cooling methods on growing pigs’ responses. Livest Sci. 2006;104:278–91.View ArticleGoogle Scholar
- Liu F, Yin J, Du M, Yan P, Xu J, Zhu X, et al. Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling. J Anim Sci. 2009;87:1941–9.PubMedView ArticleGoogle Scholar
- Gourdine JL, Mandonnet N, Giorgi M, Renaudeau D. Genetic parameters for thermoregulation and production traits in lactating sows reared in tropical climate. Animal. 2016;5:1–10.Google Scholar
- Collin A, van Milgen J, Dubois S, Noblet J. Effect of high temperature and feeding level on energy utilization in piglets. J Anim Sci. 2001;79:1849–57.PubMedView ArticleGoogle Scholar
- Kerr BJ, Yen JT, Nienaber JA, Easter RA. Influences of dietary protein level, amino acid supplementation and environmental temperature on performance, body composition, organ weights and total heat production of growing pigs. J Anim Sci. 2003;81:1998–2007.PubMedView ArticleGoogle Scholar
- Xin H, Deshazer JA. Feeding patterns of growing pigs at warm constant and cyclic temperatures. Trans ASAE. 1992;35:319–23.View ArticleGoogle Scholar
- Moretto VL, Ballen MO, Goncalves TS, Kawashita NH, Stoppiglia LF, Veloso RV, et al. Low-protein diet during lactation and maternal metabolism in rats. ISRN Obstetr Gynecol. 2011;2011:876502.Google Scholar
- Samuels SE, McAllister TA, Thompson JR. Skeletal and heart muscle protein turnover during long-term exposure to high environmental temperatures in young rats. Can J Physiol Pharmacol. 2000;78:557–64.PubMedView ArticleGoogle Scholar
- Sutherland MA, Niekamp SR, Rodriguez-Zas SL, Salak-Johnson JL. Impacts of chronic stress and social status on various physiological and performance measures in pigs of different breeds. J Anim Sci. 2006;84:588–96.PubMedView ArticleGoogle Scholar
- Tummaruk P, Tantasuparuk W, Techakumphu M, Kunavongkrit A. Effect of season and outdoor climate on litter size at birth in purebred Landrace and Yorkshire sows in Thailand. J Vet Med Sci. 2009;66:477–82.View ArticleGoogle Scholar
- Rinaldo D, Dividich JL, Noblet J. Adverse effects of tropical climate on voluntary feed intake and performance of growing pigs. Livest Prod Sci. 2000;66:223–34.View ArticleGoogle Scholar
- Myer RO, Brendemuhl JH, Bucklin RA. Effect of season on growth performance of finishing pigs fed low-protein, amino acid supplemented diets. J Appl Anim Res. 2008;34:1–8.View ArticleGoogle Scholar
- Mader TL, Frank KL, Harrington Jr JA, Hahn GL, Nienaber JA. Potential climate change effects on warm-season livestock production in the Great Plains. Climate Chang. 2009;97:529–41.View ArticleGoogle Scholar
- Bazer FW, Wu G, Johnson GA, Wang XQ. Environmental factors affecting pregnancy: endocrine disrupters, nutrients and metabolic pathways. Mol Cell Endocrinol. 2014;398:53–68.PubMedView ArticleGoogle Scholar
- Kraeling RR, Webel SK. Current strategies for reproductive management of gilts and sows in North America. J Anim Sci Biotechnol. 2015;6(1):3.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu G, Bazer FW, Datta S, Gao H, Johnson GA, Lassala A, et al. Intrauterine growth retardation in livestock: Implications, mechanisms and solutions. Arch Anim Breed. 2008;51(Special Issue 1):4–10.Google Scholar
- Wang JJ, Chen LX, Li DF, Yin YL, Wang XQ, Li P, et al. Intrauterine growth restriction affects the proteomes of the small intestine, liver and skeletal muscle in newborn pigs. J Nutr. 2008;138:60–6.PubMedView ArticleGoogle Scholar
- Oksbjerg N, Nissen PM, Therkildsen M, Møller HS, Larsen LB, Andersen M, et al. Meat Science and Muscle Biology Symposium: in utero nutrition related to fetal development, postnatal performance, and meat quality of pork. J Anim Sci. 2013;91:1443–53.PubMedView ArticleGoogle Scholar
- Rekiel A, Więcek J, Batorska M, Kulisiewicz J. Effect of sow prolificacy and nutrition on pre and postnatal growth of progeny – a review. Ann Anim Sci. 2014;14:3–15.View ArticleGoogle Scholar
- Atinmo T, Pond WG, Barnes RH. Effect of maternal energy vs. protein restriction on growth and development of progeny in swine. J Anim Sci. 1974;39:703–11.PubMedView ArticleGoogle Scholar
- Ashworth CJ, Toma LM, Hunter MG. Nutritional effects on oocyte and embryo development in mammals: implications for reproductive efficiency and environmental sustainability. Philos Trans R Soc Lond B Biol Sci. 2009;364:351–3361.View ArticleGoogle Scholar
- Johnson LJ. Maximizing feed intake of lactating sows. Compend Contin Educ Pract Vet. 1993;15:133–41.Google Scholar
- Vinsky MD, Novak S, Dixon WT, Dyck MK, Foxcroft GR. Nutritional restriction in lactating primiparous sows selectively affects female embryo survival and overall litter development. Reprod Fertil Dev. 2006;18:347–55.PubMedView ArticleGoogle Scholar
- Ashworth CJ. Effect of pre-mating nutritional status and post-mating progesterone supplementation on embryo survival and conceptus growth in gilts. Anim Reprod Sci. 1991;26:311–21.View ArticleGoogle Scholar
- Pond WG, Strachan DN, Sinha YN, Walker Jr EF, Dunn JA, Barnes RH. Effect of protein deprivation of swine during all or part of gestation on birth weight, postnatal growth rate and nucleic acid content of brain and muscle of progeny. J Nutr. 1969;99:61–7.PubMedGoogle Scholar
- Ruwe PJ, Wolverton CK, White ME, Ramsay TG. Effect of maternal fasting on fetal and placental lipid metabolism in swine. J Anim Sci. 1991;69:1935–44.PubMedView ArticleGoogle Scholar
- Noblet J, Close WH, Heavens RP. Studies on the energy metabolism of the pregnant sow. I. Uterus and mammary tissue development. Br J Nutr. 1985;53:251–65.PubMedView ArticleGoogle Scholar
- Kemp B, Soede NM, Vesseur PC, Helmond FA, Spoorenberg JH, Frankena K. Glucose tolerance of pregnant sows is related to postnatal pig mortality. J Anim Sci. 1996;74:879–85.PubMedView ArticleGoogle Scholar
- Baker DH, Becker DE, Norton HW, Sasse CE, Jensen AH, Harmon BG. Reproductive performance and progeny development in swine as influenced by feed intake during pregnancy. J Nutr. 1969;97:489–95.PubMedGoogle Scholar
- Rehfeldt C, Nissen PM, Kuhn G, Vestergaard M, Ender K, Oksbjerg N. Effects of maternal nutrition and porcine growth hormone (pGH) treatment during gestation on endocrine and metabolic factors in sows, fetuses and pigs, skeletal muscle development, and postnatal growth. Domest Anim Endocrinol. 2004;27:267–85.PubMedView ArticleGoogle Scholar
- Johnston L, Shurson J, Whitney M. Nutritional effects on fetal imprinting in swine. Proceeding of 2008 Minnesota Nutrition Conference, Owatonna, MN. 2008; pp 207-222.Google Scholar
- Anderson LL. Embryonic and placental development during prolonged inanition in the pig. Am J Physiol. 1975;229:1687–94.PubMedGoogle Scholar
- Bazer FW, Clawson AJ, Robinson OW, Vincent CK, Ulberg LC. Explanation for embryo death in gilts fed a high energy diet. J Anim Sci. 1968;27:1021–6.PubMedView ArticleGoogle Scholar
- Umbarger Show Feeds, Bargerville, IN, USA. Gestation diet for swine. http://umbargerandsons.com/hogs. Accessed on 6 Dec 2016.
- PennState Extension. Penn State College of Agricultural Sciences, College Park, PA, USA. Swine Production and management. http://extension.psu.edu/courses/swine/nutrition/gestation-and-boar-nutrition/gestation-nutrition. Accessed on 6 Dec 2016.
- Bee G. Effect of early gestation feeding, birth weight, and sex of progeny on muscle fiber characteristics of pigs at slaughter. J Anim Sci. 2004;82:826–36.PubMedView ArticleGoogle Scholar
- Nissen PM, Danielsen VO, Jorgensen PF, Oksbjerg N. Increased maternal nutrition of sows has no beneficial effects on muscle fiber number or postnatal growth and has no impact on the meat quality of the offspring. J Anim Sci. 2003;81:3018–27.PubMedView ArticleGoogle Scholar
- National Research Council (NRC). Nutrient Requirements of Swine. Washington, DC: Natl Acad Press; 1998.Google Scholar
- Sordella R, Jiang W, Chen GC, Curto M, Settleman L. Modulation of Rho GTPase signaling regulates a switch between adipogenesis and myogenesis. Cell. 2003;113:147–58.PubMedView ArticleGoogle Scholar
- Kablar B, Krastel K, Tajbakhsh S, Rudnicki MA. Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol. 2003;258:307–18.PubMedView ArticleGoogle Scholar
- Handel SE, Stickland NC. The growth and differentiation of porcine skeletal muscle fibre types and the influence of birthweight. J Anat. 1987;152:107–19.PubMedPubMed CentralGoogle Scholar
- Dwyer CM, Stickland NC, Fletcher JM. The influence of maternal nutrition on muscle fiber number development in the porcine fetus and on subsequent postnatal growth. J Anim Sci. 1994;72:911–7.PubMedGoogle Scholar
- Wang T, Liu C, Feng C, Wang X, Lin G, Zhu Y, et al. IUGR alters muscle fiber development and proteome in fetal pigs. Front Biosci (Landmark Ed). 2013;18:598–607.View ArticleGoogle Scholar
- Bérard J, Pardo CE, Bethaz S, Kreuzer M, Bee G. Intrauterine crowding decreases average birth weight and affects muscle fiber hyperplasia in piglets. J Anim Sci. 2010;88:3242–50.PubMedView ArticleGoogle Scholar
- Bérard J, Kreuzer M, Bee G. Effect of litter size and birth weight on growth, carcass and pork quality, and their relationship to postmortem proteolysis. J Anim Sci. 2008;86:2357–68.PubMedView ArticleGoogle Scholar
- Powell SE, Aberle ED. Effects of birth weight on growth and carcass composition of swine. J Anim Sci. 1980;50:860–8.PubMedView ArticleGoogle Scholar
- Schinckel AP, Einstein ME, Jungst ME, Booher S, Newman S. Evaluation of impact of pig birth weight on grow-finish performance, backfat depth and loin depth. Prof Anim Scient. 2010;26:51–69.Google Scholar
- Liu L, Chen D, Yao Y, Yu B, Mao X, He J, et al. Intrauterine growth retardation increases the susceptibility of pigs to high-fat diet-induced mitochondrial dysfunction in skeletal muscle. PLoS One. 2012;7(4):e34835.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang X, Lin G, Liu C, Feng C, Zhou H, Wang T, et al. Temporal proteomic analysis reveals defects in small-intestinal development of porcine fetuses with intrauterine growth restriction. J Nutr Biochem. 2014;25:785–95.PubMedView ArticleGoogle Scholar
- Beaulieu AD, Aalhus JL, Williams NH, Patience JF. Impact of piglet birth weight, birth order, and litter size on subsequent growth performance, carcass quality, muscle composition, and eating quality of pork. J Anim Sci. 2010;88:2767–78.PubMedView ArticleGoogle Scholar
- Bazer FW, Kim J, Ka H, Johnson GA, Wu G, Song G. Select nutrients in the uterine lumen of sheep and pigs affect conceptus development. J Reprod Develop. 2012;58:180–8.View ArticleGoogle Scholar
- Da Silva-Buttkus P, van den Hurk R, te Velde ER, Taverne MAM. Ovarian development in intrauterine growth-retarded and normally developed piglets originating from the same litter. Reproduction. 2003;126:249–58.PubMedView ArticleGoogle Scholar
- O’Gorman CW, Gonzales E, Eaton MD, Collard KA, Reyna M, Laurenz JC, et al. Foetal exposure to maternal stress influences leptin receptor gene expression during development and age at puberty in gilts. J Anim Sci. 2007;85 Suppl 2:13.Google Scholar
- Estienne MJ. Effect of birth weight on age at puberty in gilts. J Anim Sci. 2012;90(E. Suppl 2):118.Google Scholar
- Rhind SM, Rae MT, Brooks AN. Effects of nutrition and environmental factors on the fetal programming of the reproductive axis. Reproduction. 2001;122:205–14.PubMedView ArticleGoogle Scholar
- Nelson RE, Robison OW. Effects of postnatal maternal environment on reproduction of gilts. J Anim Sci. 1976;43:71–7.PubMedView ArticleGoogle Scholar
- Flowers WL. New opportunities for reproductive management. Proceedings of the London Swine Conference: Facing the New Reality. 2008, pp 31-41.Google Scholar
- Estienne MJ, Harper AF. Adult reproductive performance in high- and low-birth weight boars. J Anim Sci. 2010;88(E. Suppl 3):21.Google Scholar
- Evertts AG, Zee BM, Garcia BA. Modern approaches for investigating epigenetic signaling pathways. J Appl Physiol. 2010;109:927–33.PubMedPubMed CentralView ArticleGoogle Scholar
- Feeney A, Nilsson E, Skinner MK. Epigenetics and transgenerational inheritance in domesticated farm animals. J Anim Sci Biotechnol. 2014;5:48.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang J, Wu Z, Li D, Li N, Dindot SV, Satterfield MC, et al. Nutrition, epigenetics, and metabolic syndrome. Antioxid Redox Signal. 2012;17:282–301.PubMedPubMed CentralView ArticleGoogle Scholar
- Brosnan ME, MacMillan L, Stevens JR, Brosnan JT. Division of labour: how does folate metabolism partition between one-carbon metabolism and amino acid oxidation? Biochem J. 2015;472:135–46.PubMedView ArticleGoogle Scholar
- Dindot SV, Person R, Strivens M, Garcia R, Beaudet AL. Epigenetic profiling at mouse imprinted gene clusters reveals novel epigenetic and genetic features at differentially methylated regions. Genome Res. 2009;19:1374–83.PubMedPubMed CentralView ArticleGoogle Scholar
- Häfner SJ, Lund AH. Great expectations - Epigenetics and the meandering path from bench to bedside. Biomed J. 2016;39:166–76.PubMedView ArticleGoogle Scholar
- Golding MC, Williamson GL, Stroud TK, Westhusin ME, Long CR. Examination of DNA methyltransferase expression in cloned embryo reveals an essential role for Dnmt1 in bovine development. Mol Reprod Dev. 2011;78:306–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Sun LD, Zhao HB, Xu ZB, Liu QL, Liang W, Wang LT, et al. Phosphatidylinositol 3- kinase/protein kinase B pathway stabilizes DNA methyltransferase I protein and maintains DNA methylation. Cell Signal. 2007;19:2255–63.PubMedView ArticleGoogle Scholar
- Brown KD, Robertson KD. DNMT1 knockout delivers a strong blow to genome stability and cell viability. Nat Genet. 2007;39:289–90.PubMedView ArticleGoogle Scholar
- Wang JJ, Chen LX, Li P, Li XL, Zhou HJ, Wang FL, et al. Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J Nutr. 2008;138:1025–32.PubMedView ArticleGoogle Scholar
- Fu WJ, Haynes TE, Kohli R, Hu J, Shi W, Spencer TE, et al. Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats. J Nutr. 2005;135:714–21.PubMedGoogle Scholar
- Jobgen W, Fu WJ, Gao H, Li P, Meininger CJ, Smith SB, et al. High fat feeding and dietary L-arginine supplementation differentially regulate gene expression in rat white adipose tissue. Amino Acids. 2009;37:187–98.PubMedView ArticleGoogle Scholar
- Liu XD, Wu X, Yin YL, Liu YQ, Geng MM, Yang HS, et al. Effects of dietary L-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilical vein. Amino Acids. 2012;42:2111–9.PubMedView ArticleGoogle Scholar
- Wang WW, Wu ZL, Lin G, Hu SD, Wang B, Dai ZL, et al. Glycine stimulates protein synthesis and inhibits oxidative stress in pig small-intestinal epithelial cells. J Nutr. 2014;144:1540–8.PubMedView ArticleGoogle Scholar
- Wu G. Amino Acids: Biochemistry and Nutrition. Boca Raton: CRC Press; 2013.View ArticleGoogle Scholar
- Suryawan A, Davis TA. Regulation of protein synthesis by amino acids in muscle of neonates. Front Biosci (Landmark Ed). 2011;16:1445–60.PubMed CentralView ArticleGoogle Scholar
- Yao K, Yin YL, Chu WY, Liu ZQ, Deng D, Li TJ, et al. Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs. J Nutr. 2008;138:867–72.PubMedGoogle Scholar
- Kong XF, Wang XQ, Yin YL, Li XL, Gao HJ, Bazer FW, et al. Putrescine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells. Biol Reprod. 2014;91(5):106. 1-10.PubMedView ArticleGoogle Scholar
- Wang H, Zhang C, Wu G, Sun YL, Wang B, He BB, et al. Glutamine enhances tight-junction protein expression and modulates CRF signaling in the jejunum of weanling piglets. J Nutr. 2015;145:25–31.PubMedView ArticleGoogle Scholar
- Zhang J, Yin YL, Shu XG, Li TJ, Li FN, Tan BE, et al. Oral administration of MSG increases expression of glutamate receptors and transporters in the gastrointestinal tract of young piglets. Amino Acids. 2013;45:1169–77.PubMedView ArticleGoogle Scholar
- Li X, Bazer FW, Gao H, Jobgen W, Johnson GA, Li P, et al. Amino acids and gaseous signaling. Amino Acids. 2009;37:65–78.PubMedView ArticleGoogle Scholar
- Jia Y, Li R, Cong R, Yang X, Sun Q, Parvizi N, et al. Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter. PLoS One. 2013;8:e63855.PubMedPubMed CentralView ArticleGoogle Scholar
- Jia Y, Cong R, Li R, Yang X, Sun Q, Parvizi N, et al. Maternal low-protein diet induces gender-dependent changes in epigenetic regulation of the glucose-6-phosphatase gene in newborn piglet liver. J Nutr. 2012;142:1659–65.PubMedView ArticleGoogle Scholar
- Qasem RJ, Cherala G, D’Mello AP. Maternal protein restriction during pregnancy and lactation in rats imprints long-term reduction in hepatic lipid content selectively in the male offspring. Nutr Res. 2010;30:410–7.PubMedView ArticleGoogle Scholar
- Morrow-Tesch JL, McGlone JJ, Salak-Johnson JL. Heat and social stress effects on pig immune measures. J Anim Sci. 1994;72:2599–609.PubMedGoogle Scholar
- Pan S, Zheng Y, Zhao R, Yang X. MicroRNA-130b and microRNA-374b mediate the effect of maternal dietary protein on offspring lipid metabolism in Meishan pigs. Br J Nutr. 2013;109:1731–8.PubMedView ArticleGoogle Scholar
- Chmurzynska A, Stachowiak M, Gawecki J, Pruszynska-Oszmalek E, Tubacka M. Protein and folic acid content in the maternal diet determine lipid metabolism and response to high-fat feeding in rat progeny in an age-dependent manner. Genes Nutr. 2012;7:223–34.PubMedView ArticleGoogle Scholar
- Cong R, Jia Y, Li R, Ni Y, Yang X, Sun Q, et al. Maternal low-protein diet causes epigenetic deregulation of HMGCR and CYP7alpha1 in the liver of weaning piglets. J Nutr Biochem. 2012;23:1647–54.PubMedView ArticleGoogle Scholar
- Browne GJ, Proud CG. A novel mTOR-regulated phosphorylation site in elongation factor 2 kinase modulates the activity of the kinase and its binding to calmodulin. Mol Cell Biol. 2004;24:2986–97.PubMedPubMed CentralView ArticleGoogle Scholar
- Dever TE, Hinnebusch AG. GCN2 whets the appetite for amino acids. Mol Cell. 2005;18:141–2.PubMedView ArticleGoogle Scholar
- Jamin A, D’Inca R, Le Floc’h N, Kuster A, Orsonneau JL, Darmaun D, et al. Fatal effects of a neonatal high-protein diet in low-birth-weight piglets used as a model of intrauterine growth restriction. Neonatology. 2010;97:321–8.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Satterfield MC, Li XL, Wang XQ, Johnson GA, et al. Impacts of arginine nutrition on embryonic and fetal development in mammals. Amino Acids. 2013;45:241–56.PubMedView ArticleGoogle Scholar
- Mateo RD, Wu G, Bazer FW, Park JC, Shinzato I, Kim SW. Dietary L-arginine supplementation enhances the reproductive performance of gilts. J Nutr. 2007;137:652–56.PubMedGoogle Scholar
- Ramaekers P, Kemp B, van der Lende T. Progenos in sows increases number of piglets born. J Anim Sci. 2006;84 Suppl 1:394.Google Scholar
- Bérard J, Bee G. Effects of dietary L-arginine supplementation to gilts during early gestation on foetal survival, growth and myofiber formation. Animal. 2010;4:1680–7.PubMedView ArticleGoogle Scholar
- Ashworth CJ, Antipatis C. Micronutrient programming of development throughout gestation. Reproduction. 2001;122:527–35.PubMedView ArticleGoogle Scholar
- Tan BE, Yin YL, Kong XF, Li P, Li XL, Gao HJ, et al. L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells. Amino Acids. 2010;38:1227–35.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Johnson GA, Knabe DA, Burghardt RC, Spencer TE, et al. Important roles for L-glutamine in swine nutrition and production. J Anim Sci. 2011;89:2017–30.PubMedView ArticleGoogle Scholar
- Dolinoy DC, Huang D, Jirtle RL. Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development. Proc Natl Acad Sci U S A. 2007;104:13056–61.PubMedPubMed CentralView ArticleGoogle Scholar
- Waterland RA, Jirtle RL. Transposable elements: targets for early nutritional effects on epigenetic gene regulation. Mol Cell Biol. 2003;23:5293–300.PubMedPubMed CentralView ArticleGoogle Scholar
- Liu C, Lin G, Wang X, Wang T, Wu G, Li D, et al. Intrauterine growth restriction alters the hepatic proteome in fetal pigs. J Nutr Biochem. 2013;24:954–9.PubMedView ArticleGoogle Scholar
- Liu J, Yao Y, Yu B, Mao X, Huang Z, Chen D. Effect of maternal folic acid supplementation on hepatic proteome in newborn piglets. Nutrition. 2013;29:230–4.PubMedView ArticleGoogle Scholar
- Rezaei R, Wu ZL, Hou YQ, Bazer FW, Wu G. Amino acids and mammary gland development: nutritional implications for neonatal growth. J Anim Sci Biotechnol. 2016;7:20.PubMedPubMed CentralView ArticleGoogle Scholar
- Gonzalez-Bulnes A, Astiz S, Parraguez VH, Garcia-Contreras C, Vazquez-Gomez M. Empowering translational research in fetal growth restriction: Sheep and swine animal models. Curr Pharm Biotechnol. 2016;17:848–55.PubMedView ArticleGoogle Scholar
- Wu G, Bazer FW, Cross HR. Land-based production of animal protein: impacts, efficiency, and sustainability. Ann NY Acad Sci. 2014;1328:18–28.PubMedView ArticleGoogle Scholar
- Sun YL, Wu ZL, Li W, Zhang C, Sun KJ, Ji Y, et al. Dietary L-leucine supplementation enhances intestinal development in suckling piglets. Amino Acids. 2015;47:1517–25.PubMedView ArticleGoogle Scholar
- Sun YL. Effects of leucine on growth and expression of tissue amino acid transporters in breast-fed piglets. Ph.D. Dissertation. China Agricultural University, Beijing, China. 2015.Google Scholar
- Li XL, Bazer FW, Johnson GA, Burghardt RC, Frank JW, Dai ZL, et al. Dietary supplementation with L-arginine between days 14 and 25 of gestation enhances embryonic development and survival in gilts. Amino Acids. 2014;46:375–84.PubMedView ArticleGoogle Scholar
- Wu G. Urea synthesis in enterocytes of developing pigs. Biochem J. 1995;312:717–23.PubMedPubMed CentralView ArticleGoogle Scholar
- Mateo RD, Wu G, Moon HK, Carroll JA, Kim SW. Effects of dietary arginine supplementation during gestation and lactation on the performance of lactating primiparous sows and nursing piglets. J Anim Sci. 2008;86:827–35.PubMedView ArticleGoogle Scholar
- Wu G, Flynn NE, Knabe DA. Enhanced intestinal synthesis of polyamines from proline in cortisol-treated piglets. Am J Physiol. 2000;279:E395–402.Google Scholar
- Assaad H, Zhou L, Carroll RJ, Wu G. Rapid publication-ready MS-Word tables for one-way ANOVA. SpringerPlus. 2014;3:474.PubMedPubMed CentralView ArticleGoogle Scholar