Open Access

Effects of corn gluten feed inclusion at graded levels in a corn-soybean diet on the ileal and fecal digestibility of growing pigs

  • Gerardo Mariscal Landín1Email author,
  • Tércia Cesária Reis de Souza2 and
  • Ericka Ramírez Rodríguez1
Journal of Animal Science and Biotechnology20145:40

https://doi.org/10.1186/2049-1891-5-40

Received: 27 March 2014

Accepted: 11 August 2014

Published: 20 August 2014

Abstract

Background

This study aimed to determine the effect of the inclusion of corn gluten feed (CGF) on the apparent and standardized ileal digestibility of protein and amino acids and the apparent ileal and total tract digestibility of energy in growing pigs. The study was performed using 16 barrows (weight, 45.3 ± 4.5 kg) that were fitted with a T cannula at the terminal ileum. There were four treatments: a corn-soybean diet without CGF and three corn-soybean diets containing increasing levels of CGF (65, 130, and 195 g/kg). Data were analyzed according to a randomized complete block design, four blocks with four pigs each (one pig per treatment). The trend of the response (linear or quadratic) was determined using orthogonal contrasts, and when a linear effect was determined, a linear equation was obtained.

Results

The results showed that the inclusion up to 195 g/kg of CGF in the corn-soybean diet did not diminish the ileal digestibility (apparent and standardized) of protein and amino acids (P > 0.05), except that of phenylalanine, cystine, and proline. A linear decrease (P < 0.05) per gram of CGF added to the diet in the apparent and standardized ileal digestibility of phenylalanine (0.011 and 0.015 percentage units, respectively), cystine (0.048 and 0.043 percentage units, respectively), and proline (0.045 and 0.047 percentage units, respectively) was noted. Similarly, ileal digestibility of dry matter and energy were adversely affected (reduced by 0.028 and 0.025 percentage units, respectively, per gram of CGF increment in the diet). A significant (P < 0.05) linear reduction in total tract digestibility with increase in CGF amount in the diet was observed for energy (0.027 percentage units), dry matter (0.027 percentage units), crude protein (0.020 percentage units), and neutral detergent fiber (0.041 percentage units) per gram of CGF added to the diet.

Conclusion

CGF did not affect the ileal digestibility of protein and most amino acids but reduced the ileal and total tract digestibility of energy.

Keywords

Amino acids Corn gluten feed Energy Ileal digestibility Pigs Total tract digestibility

Background

Corn (Zea mays) is the most harvested cereal worldwide [1]. Most of the corn produced is processed to extract flour, syrup, sweeteners, starches, oils, and ethanol. When wet milling is used to process corn, the germ is separated from the kernel, processed to obtain edible oil, and the resulting flour is mixed with the bran to produce corn gluten feed (CGF) [2], which has traditionally been considered a protein feed [3], although it is rich in fiber [4, 5]. Therefore, the inclusion of CGF in pig diets has been limited, since fiber can affect directly [6, 7] and indirectly [8, 9] the digestibility of amino acids by increasing endogenous protein losses. Thus, this study aimed to determine the effects of inclusion of CGF in a corn-soybean meal diet on the apparent and standardized ileal digestibility of amino acids and apparent total tract digestibility of energy in growing pigs. We hypothesized that the inclusion of CGF decreases the ileal digestibility of amino acids and energy and the total tract digestibility of energy.

Materials and methods

This study was approved by the Scientific Associate Technical Group Committee of CENID Physiology. The animals used in this study were cared for in accordance with the guidelines issued by the Mexican Official Standard for the Production, Protection and Use of Lab Animals [10] and the guidelines of the International Guiding Principles for Biomedical Research Involving Animals [11]. The study was performed at the experimental farm of CENID-Physiology.

Animals

Sixteen barrows (Duroc × Landrace) weighing 45.3 ± 4.5 kg were used. The animals were placed in individual metabolic cages provided with a self-feeder and a low-pressure drinking nipple in a temperature controlled room at 20 ± 2°C. Animals were fed twice daily at 0800 h and 1800 h and had free access to water. A T cannula was fitted at the terminal ileum of each animal as previously described [12]. After surgery, therapeutic treatment (penicillin, 600,000 IU; streptomycin, 750 mg; oxytetracycline, 500 mg) was administered for 3 d; the post-surgery period lasted 21 d. From the second day after the post-surgery period, pigs began to receive 100 g of feed, which was increased by 100 g/day until the feed intake reached the level before surgery. During the experimental period the pigs were fed at 2.5-times the maintenance requirement of digestible energy of 460 kJ/kg BW0.75[13]. Pigs had free access to water.

Experimental diets

The experimental diets were produced using corn, soybean meal, and CGF (Table 1). Four diets were formulated (Table 2) with increasing levels of CGF (0, 65, 130, and 195 g/kg of diet) at the expenses of corn and soybean meal. Corn oil was included at a rate of 40 g/kg. Salt, vitamins, and minerals were included at the levels that met or exceeded the National Research Council (NRC) nutritional requirements [5]; chromic oxide was added at a rate of 3 g/kg of feed as an inert marker [14]. The total fecal collection was performed daily for 5 d to achieve the required chromic oxide rate. Ferric oxide was added (3 g/kg of diet) to the first meal of the fifth day (to mark the start of the collection period) as well as to the first meal of the eleventh day (to mark the end of the collection period) [15].
Table 1

Chemical composition of raw materials as fed-basis (g/kg)

Item

SBM1

YC

CGF

Dry matter

899.5

900.3

891.2

Crude protein

468.1

108.5

208.4

NDF2

89.5

119.7

338.7

ADF3

57.4

45.0

106.5

Amino acids

   

Alanine

24.8

7.2

17.1

Aspartic acid

67.5

9.7

17.3

Arginine

42.6

8.2

12.1

Cystine

8.3

2.7

4.3

Glutamic acid

111.7

20.8

45.3

Glycine

24.1

6.1

12.2

Histidine

10.1

4.7

7.1

Isoleucine

24.5

3.3

7.6

Leucine

44.2

10.1

23.8

Lysine

30.3

2.8

5.4

Methionine

7.8

2.0

3.4

Phenylalanine

28.9

4.7

9.7

Proline

31.1

8.8

16.6

Serine

30.7

5.7

11.5

Threonine

27.9

5.6

12.1

Tyrosine

20.8

3.9

8.1

Valine

25.5

5.6

12.1

1Raw materials: SBM = soybean meal, YC = yellow corn, CGF = corn gluten feed.

2NDF = neutral detergent fiber.

3ADF = acid detergent fiber.

Table 2

Composition and analyzed nutrient composition of experimental diets, as fed-basis (g/kg)

g CGF/kg feed

0

65

130

195

Yellow corn

762.9

716.9

671.2

625.6

Soybean meal

155.9

136.7

117.4

98

Corn gluten feed

 

65.7

131

196.4

Corn oil

40.0

40.0

40.0

40.0

Calcium carbonate

17.9

19.2

20.5

21.9

Dicalcium phosphate

10.4

8.4

6.3

4.1

Salt

3.5

3.5

3.5

3.5

L-Lysine HCl

2.5

2.4

2.4

2.3

Tryptosine

1.4

1.9

2.4

2.9

Threonine

0.2

   

Vitamins1

1.6

1.6

1.6

1.6

Minerals2

0.7

0.7

0.7

0.7

Chromium oxide

3.0

3.0

3.0

3.0

Chemical analysis

    

Dry matter

904.3

908.3

904.9

907.1

Protein

128.7

127.7

140.2

135.7

Energy, MJ

17.6

17.6

17.6

17.2

NDF

103.5

121.8

138.3

149.2

Amino acids

    

Alanine

7.4

8.5

9.8

9.2

Arginine

8.8

9.8

11.0

9.6

Aspartic acid

13.5

14.0

15.5

13.3

Cystine

2.9

2.9

2.7

2.4

Glutamic acid

24.1

27.1

31.6

28.0

Glycine

6.6

7.2

8.1

7.4

Histidine

3.9

4.6

4.9

5.0

Isoleucine

2.6

4.3

5.3

5.0

Leucine

9.8

12.3

14.4

13.3

Lysine

9.3

9.8

9.8

9.4

Methionine

1.8

1.7

1.7

1.4

Phenylalanine

5.3

6.2

7.1

6.1

Proline

8.3

11.8

12.2

8.4

Serine

7.3

7.6

8.8

7.5

Threonine

6.4

6.9

7.8

7.1

Tyrosine

4.3

5.2

5.8

5.0

Valine

3.8

6.3

7.4

7.3

1Provided per kg piglet diet: Cl, 1.65 g; Na, 0.87 g; Cu, 7.7 mg; Fe, 89.25 mg; Mn, 19.98 mg; Se, 0.087 mg; I, 0.053 mg.

2Provided per kg piglet diet: vitamin A, 6600 IU; vitamin D, 660 IU; vitamin E, 100 IU; choline, 350 mg; niacin, 54 mg; pantothenic acid, 13.15 mg; riboflavin, 2.2 mg; B12, 36 μg.

NDF = neutral detergent fiber.

Sample collection

The experimental period lasted 12 d; this included 5 d for adaptation to the diet, 5 d for the collection of feces, and 2 d for the collection of ileal digesta. Ileal digesta was collected in plastic bags (length, 11 cm; width, 5 cm) containing 10 mL of 0.2 mol/L solution of HCl to block any bacterial activity. Bags were attached to the barrel of the cannula by using a rubber band. Ileal digesta was collected continuously over the course of 12 h each day. When the bags were filled, they were transferred to a container and frozen at -20°C until lyophilization. All fecal samples were collected, frozen, and kept at -20°C. At the end of the experimental period, the feces were defrosted and homogenized to obtain 10% of the weight as a final sample for lyophilizing.

Chemical analysis

Ileal digesta and feces samples were lyophilized and ground in a laboratory mill by using a 0.5-mm mesh (Arthur H. Thomas Co., Philadelphia, PA). Raw materials, experimental diets, ileal digesta, and feces were analyzed for dry matter (DM) and crude protein (CP) according to methods 934.01 and 976.05 of the Association of Official Agricultural Chemists (AOAC) [16]; neutral detergent fiber (NDF), according to van Soest [17]; and energy, by using an oxygen bomb calorimeter (model 1281; Parr, Moline, IL). Chromic oxide levels in the diets, ileal digesta, and feces were determined according to Fenton and Fenton [14]. Amino acid analysis was performed following method 994.12 of the AOAC [16]; samples were hydrolyzed in 6 mol/L HCl at 110°C for 24 h. Methionine and cystine were oxidized with performic acid before the analysis. The amino acid analysis was performed according to the method reported by Henderson et al. [18] by using a high-performance liquid chromatography (HPLC) model (1100; Hewlett Packard).

Data analysis

Apparent ileal or total tract digestibility (AID or ATTD) were estimated using the equation proposed by Fan and Sauer [19].
AID = 1 ID × AF / AD × IF × 100 ,

where AID% is the apparent (ileal or total) digestibility of a nutrient in the diet, ID is the marker concentration in the diet (mg/kg of DM), AF is the concentration of nutrient in the ileal digesta or feces (mg/kg of DM), AD is the concentration of the nutrient in the diet (mg/kg of DM), and IF is the marker concentration in the ileal digesta or feces (mg/kg of DM).

The standardized ileal digestibility (SID) was obtained using the formula proposed by Furuya and Kaji [20].
SID = AID + Endogenous / Dietary Content

where SID is the standardized ileal digestibility of a nutrient, AID is the coefficient of apparent ileal digestibility of a nutrient, and Endogenous is the endogenous ileal losses of a nutrient in mg/kg of dry matter intake. The calculations were performed using endogenous values reported by Mariscal-Landin and Reis de Souza [21]. Dietary Content is the amount of nutrient consumed in mg/kg of dry matter intake.

Statistical analysis

Data were analyzed according to a randomized complete block design [22] by using the general linear model (GLM) procedure of statistical analysis system (SAS) [23]: four blocks with four pigs each (one pig per treatment). Each pig was the experimental unit, and an alpha value of 0.05 was used to assess the significance. The trend of the response (linear or quadratic) was determined using orthogonal contrasts [22]. When a linear effect was determined, a linear equation was obtained using the regression (REG) procedure of SAS [23].

Results

Apparent ileal digestibility

The results of apparent ileal digestibility are shown in Table 3. The inclusion of CGF significantly reduced (P < 0.05) the AID of dry matter; there was a reduction of 5.7 percentage units between the diet without CGF and the diet containing 195 g of CGF (87.7% vs. 82.0%). This adverse effect was also observed in energy digestibility, which was reduced by 4.9 percentage units (from 88.8 to 83.9 in the diets with 0 or 195 g of CGF, respectively). The digestibility of phenylalanine, cystine and proline decreased linearly (P < 0.05) in response to CGF increment in the diet. The average reduction in ileal digestibility of amino acids was 0.031 percentage units per gram of CGF included in the diet. Cystine digestion was the most affected (0.048 percentage units) and phenylalanine, the least (0.011 percentage units). The digestibility of the other amino acids was not affected by the inclusion of CGF at the levels used in this study.
Table 3

Apparent ileal digestibility (AID) coefficients (%) of experimental diets

g CGF/kg feed

0

65

130

195

SEM

Dry matterA

87.7

83.8

82.8

82.0

0.66

Protein

84.9

81.4

81.7

80.7

0.98

EnergyA

88.8

86.0

84.6

83.9

0.66

Amino acids

     

Alanine

82.4

81.1

80.3

81.5

1.31

Arginine

93.1

91.9

91.0

89.5

0.86

Aspartic acid

86.1

83.1

83.1

81.5

1.05

CystineA

86.1

85.1

81.8

76.7

1.22

Glutamic acid

88.7

88.1

87.7

86.9

0.92

Glycine

80.2

79.3

77.5

76.3

1.38

Histidine

87.5

86.7

85.7

85.7

0.91

Isoleucine

79.0

83.5

83.1

83.1

1.63

Leucine

87.4

87.4

87.4

86.9

1.03

Lysine

92.7

90.8

90.6

90.6

0.67

Methionine

83.3

82.4

82.1

78.2

1.20

PhenylalanineA

93.7

92.1

92.0

91.4

0.30

ProlineA

87.5

89.1

83.9

79.6

1.27

Serine

85.5

82.8

82.7

81.1

1.18

Threonine

81.9

77.8

77.5

77.2

1.52

Tyrosine

88.2

88.1

87.3

85.9

0.94

Valine

79.8

85.2

84.1

83.5

1.57

ALinear effect (P < 0.05).

Standardized ileal digestibility

The standardized ileal digestibility of amino acids is shown in Table 4. In general, the inclusion of CGF in the diet did not affect (P > 0.05) the SID. However, a linear decrease in SID of phenylalanine (0.015 percentage units; from 98.7 to 95.8), cystine (0.043 percentage units, from 91.4 to 83.1), and proline (0.047 percentage units, from 103.7 to 94.9) was noted per gram inclusion of CGF. There was no significant reduction in the digestibility of the other amino acids (P > 0.05).
Table 4

Standardized ileal digestibility (SID) coefficients (%) of experimental diets

g CGF/kg feed

0

65

130

195

SEM

Protein

93.7

90.3

89.9

89.1

0.98

Amino acids

     

Alanine

87.6

85.7

84.3

85.7

1.31

Aspartic acid

91.2

88.0

87.4

86.6

1.05

Arginine

97.1

95.5

94.7

92.7

0.86

CystineA

91.4

90.3

87.3

83.1

1.22

Glutamic acid

91.9

91.0

90.1

89.7

0.92

Glycine

92.0

89.9

86.9

86.8

1.38

Histidine

91.8

90.3

89.2

89.1

0.91

Isoleucine

90.3

90.5

89.0

88.9

1.63

Leucine

92.5

92.0

90.9

90.6

1.03

Lysine

96.8

94.2

94.2

93.5

0.67

Methionine

87.2

86.2

86.6

83.2

1.20

PhenylalanineA

98.7

96.5

95.8

95.8

0.30

ProlineA

103.7

100.5

95.5

94.9

1.27

Serine

92.4

89.3

88.4

87.9

1.18

Threonine

90.8

86.0

85.6

84.5

1.52

Tyrosine

91.4

90.7

89.7

88.7

0.94

Valine

90.3

91.6

89.5

89.0

1.57

ALinear effect (P < 0.05).

Apparent total tract digestibility

The ATTD of dry matter, protein, energy, and NDF diminished (P < 0.05) linearly with an increase in CGF concentration in the diet (Table 5). The effect of CGF inclusion on ATTD was net and clear. The reduction of digestibility of dry matter and energy was 6.7 percentage units; for protein, 6.3 percentage units; and for NDF, 11.3 percentage units. The linear equations are shown in Table 6; a decrease of 0.02 to 0.03 percentage units in the ATTD of DM, CP, and energy per gram of CGF included in the diet and a decrease of 0.041 percentage units in the ATTD of NDF were noted per gram of CGF included in the diet.
Table 5

Apparent total tract digestibility (ATTD) coefficients (%) of experimental diets

g CGF/kg feed

0

65

130

195

SEM

Dry matterA

88.6

81.3

83.7

81.9

0.36

ProteinA

86.9

76.9

83.4

80.6

0.59

EnergyA

88.7

81.4

84.1

82.0

0.36

NDFA

62.5

47.8

55.2

51.2

1.42

ALinear effect (P < 0.05).

NDF = neutral detergent fiber.

Table 6

Linear relationships between corn gluten feed (CGF) inclusion and ileal and total tract digestibility coefficients

Apparent ileal digestibility

  

Dry matter

DM = 86.77 (±1.02) – 0.028 (±0.008) gCGF1

r2 = 0.86

Energy

E = 88.22 (±0.96) – 0.025 (±0.008) gCGF

r2 = 0.92

Phenylalanine

Phe = 93.31 (±0.46) – 0.011 (±0.004) gCGF

r2 = 0.84

Cystine

Cys = 87.15 (±1.90) – 0.048 (±0.016) gCGF

r2 = 0.92

Proline

Pro = 89.36 (±1.89) – 0.045 (±0.016) gCGF

r2 = 0.78

Standardized ileal digestibility

  

Phenylalanine

Phe = 98.13 (±0.51) – 0.015 (±0.004) gCGF

r2 = 0.78

Cystine

Cys = 92.19 (±1.88) – 0.043 (±0.015) gCGF

r2 = 0.94

Proline

Pro = 103.20 (±1.84) – 0.047 (±0.015) gCGF

r2 = 0.93

Apparent total tract digestibility

  

Dry matter

DM = 86.52 (±1.16) – 0.027 (±0.010) gCGF

r2 = 0.47

Protein

CP = 83.85 (±1.74) – 0.020 (±0.014) gCGF

r2 = 0.14

Energy

E = 86.64 (±1.20) – 0.027 (±0.010) gCGF

r2 = 0.46

NDF

NDF = 58.13 (±2.97) – 0.041 (±0.015) gCGF

r2 = 0.29

1gCGF = grams of corn gluten feed added to the diet.

NDF = neutral detergent fiber.

Discussion

Fiber has been defined as “vegetal compounds, from carbohydrate nature and resistant to digestive enzymes”; it is classified according to solubility as soluble fiber (β-glucans, gums, and mucilages) and insoluble fiber (cellulose and most hemicelluloses) [24]. CGF is a mixture of corn structures that remain after the removal of most starch and gluten germ [2]. Approximately, two-thirds of these corn structures are fibrous structures, and one-third consists of soluble compounds [25]. The fiber obtained from corn is essentially insoluble (99% of the total fiber) and represents over 30% of the dry matter [25]; the CGF used in this study had 338.7 g NDF/kg. Insoluble fiber is known to cause endogenous protein losses and reduce the AID of protein and amino acids because it increases mucin secretion as well as mucosal cell shedding via its abrasive effect [8, 26, 27]. The protein content of CGF is not contained in the fibrous part (bran). It is present in the soluble fraction that is mixed with bran at the end of the milling process [2]; therefore, fiber does not block the enzyme access to proteins as is noted in wheat bran, in which a high proportion of protein is present in the aleurone cells [28]. Furthermore, several authors [2931] have suggested that insoluble fiber has a minor effect on the ileal digestibility of amino acids and protein. Both findings (protein is not present in the fibrous part and the minor effect of insoluble fiber on the ileal digestibility of protein) could explain the almost null effect of CGF on the ileal digestibility of protein and amino acids. The decrease in the ileal digestibility of proline could be because of the proteins present in the fibrous fraction; these proteins are rich in extensine—a proline-rich protein that resembles collagen—and are closely linked to the cellulose fraction [3234]. The negative effect on the ileal digestibility of dry matter and energy was due to an increase in the levels of non-digestible compounds (insoluble fiber) after treatment with CGF; as mentioned before, two-thirds of the CGF is insoluble fiber, and hence, it dilutes the digestible dry matter and energy as has been reported previously [35].

In the large intestine, the undigested food (mainly non-starch polysaccharides and protein) is fermented by microorganisms [36]. This is a long process, but the insoluble fiber decreases the residence time of digesta in the cecum and colon, and this effect is related to the fiber content of the diet [24, 29, 35]. Insoluble fibers are resistant to fermentation; therefore, they play a major role in fecal bulking, unlike soluble fibers that are almost completely fermented and have little effect on increasing fecal bulk [37]. This limited fermentation of insoluble fibers by intestinal bacteria reduces the total digestibility of protein and energy [31, 35, 38]; consequently, the digestible energy is low in diets containing CGF. Furthermore, fibers increase the production of mucin, an almost indigestible protein, thereby increasing protein excretion in the feces [39] and lowering protein digestion. As discussed before, the inclusion of CGF in diets has a mild effect on the ileal digestibility of amino acids; however, it adversely affects energy digestibility. Few studies have quantitatively investigated the adverse effect of NDF on energy digestibility; the findings reported in this study are in agreement with those reported by Dégen [31]. However, in sows, fiber plays an important role in avoiding stereotyped conducts, and thus, sows are able to obtain more energy from fiber than growing pigs [24, 40, 41].

Conclusions

CGF did not affect the apparent and standardized ileal digestibility of protein and most amino acids, except that of phenylalanine, cystine, and proline. However, it linearly decreased the ileal digestibility of energy and the total tract digestibility of protein and energy.

Abbreviations

CGF: 

Corn gluten feed

AID: 

Apparent ileal digestibility

SID: 

Standardized ileal digestibility

ATTD: 

Apparent total tract digestibility

NDF: 

Neutral detergent fiber.

Declarations

Acknowledgments

This study was funded in part by the Ministry of Agriculture, Livestock and Fishing (SAGARPA) of Mexico and The National Council for Science and Technology (CONACYT) of Mexico via the financial support provided to Research Project SAGARPA-CONACYT 2003-2-169.

Authors’ Affiliations

(1)
Centro Nacional de Investigación en Fisiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
(2)
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Santiago de Querétaro

References

  1. FAO: FAOSTAT Organización de las Naciones Unidas para la Alimentación y la Agricultura. 2010Google Scholar
  2. Blasi DA, Drouillard J, Brouk MJ, Montgomory SP:Corn Gluten Feed, Composition and Feeding Value for Beef and Dairy Cattle. Corn Gluten Feed, Composition and Feeding Value for Beef and Dairy Cattle. 2001, Manhattan: Kansas State University, 14 pages.Google Scholar
  3. Yen JT, Brooks JD, Jensen AH: Metabolizable energy value of corn gluten feed. J Anim Sci. 1974, 39: 335-337.PubMedGoogle Scholar
  4. INRA: Tables de composition et de valeur nutritive des matières premières destinées aux animaux d’élevage. Porcs, volailles, bovins, ovins, caprins, lapins, chevaux, poissons. 2002, Paris, France: Institut National de la Recherche AgronomiqueGoogle Scholar
  5. NRC: Nutrient requirements of swine. 1998, Washington, DC: The National Academy Press, 10Google Scholar
  6. Ma QG, Metzler BU, Eklund M, Ji C, Mosenthin R: The effects of cellulose, pectin and starch on standardized ileal and apparent total tract amino acid digestibilities and bacterial contribution of amino acids in feces of growing pigs. Asian-Aust J Anim Sci. 2008, 21: 873-882. 10.5713/ajas.2008.70478.View ArticleGoogle Scholar
  7. Myrie SB, Bertolo RF, Sauer WC, Ball RO: Effect of common antinutritive factors and fibrous feedstuffs in pig diets on amino acids digestibilities with special emphasis in threonine. J Anim Sci. 2008, 86: 609-619.View ArticlePubMedGoogle Scholar
  8. Mariscal-Landín G, Reis de Souza TC, Hernández DAA, Escobar GK: Pérdidas endógenas de nitrógeno y aminoácidos en cerdos y su aplicación en la estimación de los coeficientes de digestibilidad ileal de la proteína y aminoácidos de las materias primas. Téc Pecu Méx. 2009, 47: 371-388.Google Scholar
  9. Schulze H, van Leeuwen P, Verstegen MWA, Huisman J, Souffrant WB, Ahrens F: Effect of level of dietary neutral detergent fiber on ileal apparent digestibility and ileal nitrogen losses in pigs. J Anim Sci. 1994, 72: 2362-2368.PubMedGoogle Scholar
  10. Diario Oficial de la Federación: Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Norma Oficial Mexicana NOM-062-ZOO-1999. Diario Oficial de la Federación, Segunda Sección, Miércoles 22 de Agosto. 2001Google Scholar
  11. International guiding principles for biomedical research involving animals. The development of science-based guidelines for laboratory animal care-NCBI Bookshelf. [http://cioms.ch/publications/guidelines/1985_texts_of_guidelines.htm]
  12. de Reis de Souza TC, Mar BB, Mariscal LG: Canulación de cerdos posdestete para pruebas de digestibilidad ileal: Desarrollo de una metodología. Téc Pecu Méx. 2000, 38: 143-150.Google Scholar
  13. INRA: L’alimentation des animaux monogastriques: porc, lapin, volailles. 1984, Paris, France: Institut National de la Recherche AgronomiqueGoogle Scholar
  14. Fenton TW, Fenton M: An improved procedure for determination of chromic oxide in feed and feces. Can J Anim Sci. 1979, 59: 631-634. 10.4141/cjas79-081.View ArticleGoogle Scholar
  15. Adeola O: Digestion and balance technique in pigs. Swine Nutrition. Edited by: Lewis AJ, Southern LL. 2001, Boca Raton, USA: CRC Press, 903-916. 2Google Scholar
  16. AOAC: Official Methods of Analysis. 2000, Arlington, VA. USA: Assoc. Offic. Anal. Chem, 17Google Scholar
  17. van Soest PJ, Robertson JB, Lewis BA: Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991, 74: 3583-3597. 10.3168/jds.S0022-0302(91)78551-2.View ArticlePubMedGoogle Scholar
  18. Henderson JH, Ricker RD, Bidlingmeyer BA, Woodward C: Rapid, accurate and reproducible HPLC analysis of amino acids. Amino acid analysis using Zorbax Eclipse AAA columns and the Agilent 1100 HPLC. Agilent technologies. 2000, 10 pag. Agilent technologies homepage [http://www.agilent.com/chem/supplies]Google Scholar
  19. Fan MZ, Sauer WC: Determination of apparent ileal amino acid digestibility in barley and canola meal for pigs with the direct, difference, and regression methods. J Anim Sci. 1995, 73: 2364-2374.PubMedGoogle Scholar
  20. Furuya S, Kaji Y: Estimation of the true ileal digestibility of amino acids and nitrogen from their apparent values for growing pigs. Anim Feed Sci Technol. 1989, 26: 271-285. 10.1016/0377-8401(89)90040-0.View ArticleGoogle Scholar
  21. Mariscal-Landín G, Reis de Souza TC: Endogenous ileal losses of nitrogen and amino acids in pigs and piglets fed graded levels of casein. Arch Anim Nutr. 2006, 60: 454-466. 10.1080/17450390600973642.View ArticlePubMedGoogle Scholar
  22. Steel RGD, Torrie JH: Principles and procedures of statistics. A Biometrical approach. 1980, New York: McGraw-Hill, 2Google Scholar
  23. SAS version 9.2: Book Statistical Analysis Systems Institute User’s guide. 2008, Cary NC: SAS Institute Inc, 92Google Scholar
  24. Le Gall M, Montagne L, Meunier-Salaün MC, Noblet J: Valeurs nutritives des fibres, conséquences sur la santé du porcelet et le bien-être de la truie. INRA Prod Anim. 2009, 22: 17-24.Google Scholar
  25. Kawauchi IM, Sakomura NK, Vasconcellos RS, de-Oliveira LD, Gomes MOS, Loureiro BA, Carciofi AC: Digestibility and metabolizable energy of maize gluten feed for dogs as measured by two different techniques. Anim Feed Sci Technol. 2011, 169: 96-103. 10.1016/j.anifeedsci.2011.05.005.View ArticleGoogle Scholar
  26. Mariscal-Landín G, Sève B, Collèaux Y, LeBreton Y: Endogenous amino nitrogen collected from pigs with end to end ileorectal anastomosis is affected by the method of estimation and altered by dietary fiber. J Nutr. 1995, 125: 136-146.PubMedGoogle Scholar
  27. Reverter M, Lindberg JE: Ileal digestibility of amino acids in pigs given a barley-based diet with increasing inclusion of Lucerne meal. Anim Sci. 1998, 67: 131-138. 10.1017/S1357729800009863.View ArticleGoogle Scholar
  28. Laubin B, Lullien-Pellerin V, Nadaud I, Gaillard-Martinie B, Chambon C, Branlard G: Isolation of the wheat aleurona layer for 2D electrophoresis and proteomic analysis. J Cereal Sci. 2008, 48: 709-714. 10.1016/j.jcs.2008.03.004.View ArticleGoogle Scholar
  29. Bach Knudsen KE: The nutritional significance of “dietary fibre” analysis. Anim Feed Sci Technol. 2001, 90: 3-20. 10.1016/S0377-8401(01)00193-6.View ArticleGoogle Scholar
  30. Li S, Sauer WC, Hardin RT: Effect of dietary fibre level on amino acid digestibility in young pigs. Can J Anim Sci. 1994, 74: 327-333. 10.4141/cjas94-044.View ArticleGoogle Scholar
  31. Dégen L, Halas B, Babinszky L: Effect of dietary fibre on protein and fat digestibility and its consequences on diet formulation for growing and fattening pigs: A review. Acta Agric Scand Sect A Anim Sci. 2007, 57: 1-9.Google Scholar
  32. Sun T, Li S, Ren H: Profilin as a regulator of the membrane-actin cytoskeleton interface in plant cells. Front Plant Sci. 2013, 4: 512.PubMed CentralView ArticlePubMedGoogle Scholar
  33. Williamson MP: The structure and function of proline-rich regions in proteins. Biochem J. 1994, 297: 249-260.PubMed CentralView ArticlePubMedGoogle Scholar
  34. Bjergegaar C, Sørensen H, Sørensen S: Dietary fibres-important parts of high quality food and feeds. J Anim Feed Sci. 1997, 6: 145-161.Google Scholar
  35. Ngoc T, Len N, Lindberg J: Impact of fibre intake and fibre source on digestibility, gut development, retention time and growth performance of indigenous and exotic pigs. Animal. 2013, 7: 736-745. 10.1017/S1751731112002169.View ArticlePubMedGoogle Scholar
  36. Caspar W: The role of dietary fibre in the digestive physiology of the pig. Anim Feed Sci Technol. 2001, 90: 21-33. 10.1016/S0377-8401(01)00194-8.View ArticleGoogle Scholar
  37. Wong J, Jenkins D: Carbohydrate digestibility and metabolic effects. J Nutr. 2007, 137: 2539S-2546S.PubMedGoogle Scholar
  38. Hansen MJ, Chwalibog A, Tausson AH, Sawosz E: Influence of different fibre sources on digestibility and nitrogen and energy balances in growing pigs. Arch Anim Nutr. 2006, 60: 390-401. 10.1080/17450390600884385.View ArticlePubMedGoogle Scholar
  39. Montagne L, Piel C, Lallès JP: Effect of Diet on Mucin Kinetics and Composition: Nutrition and Health Implications. Nutr Rev. 2004, 62: 105-114.View ArticlePubMedGoogle Scholar
  40. Shi XS, Noblet J: Digestible and metabolizable energy values of ten feed ingredients in growing pigs fed ad libitum and sows fed at maintenance level; comparative contribution of the hindgut. Anim Feed Sci Technol. 1993, 42: 223-236. 10.1016/0377-8401(93)90100-X.View ArticleGoogle Scholar
  41. Kerr BJ, Shurson GC: Strategies to improve fiber utilization in swine. J Anim Sci Biotechnol. 2013, 4: 11-10.1186/2049-1891-4-11.PubMed CentralView ArticlePubMedGoogle Scholar

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