Fig. 8
From: Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams
Effect of vitamin E nanoemulsion (NE) on: a) ROS production and b) lipid peroxidation. Plots show the quadruple interaction of treatments × oxidative stress × incubation temperature × incubation time for the flow cytometry analysis of reactive oxygen species (median fluorescence of H2DCFDA in PI− sperm) and lipid peroxidation (median green fluorescence of BODIPY C11). a–d (P < 0.05) indicates significant differences among hours of incubation within each treatment and temperature of incubation under oxidative stress (orange line). *(P < 0.05) indicate differences between oxidative stress and non-oxidative stress treatments within each temperature and time of incubation