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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: MiR-146a-5p targeting SMAD4 and TRAF6 inhibits adipogenensis through TGF-β and AKT/mTORC1 signal pathways in porcine intramuscular preadipocytes

Fig. 3

MiR-146a-5p inhibitor promotes porcine intramuscular preadipocyte proliferation. a EdU staining assay. Porcine intramuscular preadipocyte in the S-phase were stained with EdU in red, and cell nuclei were dyed with Hoechst in blue. b Quantification ratio of EdU-positive cells/total cells. c CCK-8 analysis after treatment with miR-146a-5p inhibitor during porcine intramuscular preadipocyte proliferation. d Cell cycle analysis of preadipocyte by flow cytometry. e Statistical results of flow cytometry. f Interference efficiency of miR-146a-5p inhibitor after transfection for 24 h. g RT-qPCR was used to detect the cell cycle genes cyclin B, cyclin D, cyclin E, and p21. h Western blot analysis of cyclin E, cyclin D, PCNA and p21 after transfection with miR-146a-5p inhibitor. i Protein quantitative analysis of cyclin E, cyclin D, PCNA and p21. Values are expressed as the mean ± SEM (n = 3). *, P < 0.05, versus INC

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891