The ultra-sonication of minerals in swine feed
© Saleh et al. 2015
Received: 23 November 2014
Accepted: 17 June 2015
Published: 4 August 2015
A sample preparation method based on ultrasound assisted-extraction (UAE) of Ca, Mg and P from swine feed has been described. The experiment was performed to cover the variables influencing the sonication process and, the method validation using standard reference material. Final solutions obtained upon sonication were analyzed by flame atomic absorption spectrometry (for Ca and Mg) and by UV–vis spectrophotometry (for P). The best conditions for metal extraction were as follows: sample mass: 100 mg in 20 mL 0.10 mol/L HCl, a particle size: <60 μm, sonication time: 5 cycles of 10 s and ultrasound power: 102 W. The UAE method was applied in digestibility assays in different piglet feeds and their results showed that it is highly comparable (P > 0.05) to the other methods used for such purposes, as block digestion, and offered a Ca, Mg and P method of quantification limit of 10.6, 12.4 and 14 mg/kg, respectively. The major advantages of the UAE method compared to other methods are the high treatment rate, low reagent usage in the extracts and, it does not generate toxic residues that might negatively affect human health and the environment, accompanied by good precision and accuracy.
KeywordsDigestibility assay Green analytical chemistry Macronutrients Piglet Ultrasound-assisted extraction
The animal feed industry adopted the strategy of using more digestible ingredients in the formulations to minimize the inclusion of minerals and maximize the efficiency of nutrient used by the animal while minimizing the negative effects of weaning, the critical period in pig farms, ensuring the performance desirable at this stage of the piglet production, and even contributing to the reduction of environmental impact caused by its waste [1, 2].
However, the use of external markers (Cr2O3) for the estimation of the apparent digestibility coefficient of nutrients metabolized by the animal is problematic due to the expense of the large amounts of it administered to obtain analytical results and appropriate variations in the research results, mainly due to analytical variability [3–5]. Moreover, the quantification of Cr2O3 in feed and feces presents difficulties in sample preparation procedures, which plays a key role in determining the success of subsequent analysis.
The mineralization of samples is performed by slow heating in digestion blocks, using a nitric-perchloric mixture which involves the use of high temperatures or corrosive reagents, usually under pressure, which is a tedious and time-consuming treatment using concentrated and harmful reagents that demand very stringent safety conditions. The resulting extracts are acid solutions containing the inorganic nutrients and the dichromate ions (Cr2O7 2−), a highly toxic species that negatively affects human health and contaminates the environment .
Because of the negative effects of this procedure, the development of new methodologies that allow the safe quantification of inorganic nutrients in studies of animal nutrition has become crucial, mainly due to the importance of calcium, magnesium and phosphorus in biochemical processes. The use of ultrasound-assisted extraction (UAE) for the separation of nutrients from biological samples is presented as promising, mainly because the energy released during acoustic cavitation offers excellent prospects for sample preparation [7, 8], such as low volumes of solvent needed, reduction in analytical time, and the possibility of developing fully automated methods, allowing the elimination of acid mineralization [9–13], and hence contribute to the achievement of the green principles in analytical chemistry.
Due to the above, this study evaluated the efficiency of ultrasound-assisted extraction as an alternative to acid mineralization method in extracting calcium, magnesium and phosphorus from piglet feed followed by determination of the Ca and Mg concentrations by flame atomic absorption spectrometry (FAAS) and the P concentrations by ultraviolet–visible spectrophotometry (UV–vis) and, the subsequent estimation of the apparent digestibility coefficient of these nutrients.
Materials and methods
The research project was conducted at UNESP (São Paulo State University) at the Faculty of Veterinary Medicine and Animal Science, Botucatu campus, with the approval by the Animal Ethics Committee from this institution (protocol number 114/2006 – CEUA) and, in accordance with the directive 86/609/EEC.
Housing, animals, diets and experimental design
The digestibility experiments were conducted in the experimental facilities of São Paulo State University (UNESP) at Botucatu. A total of 90 crossbred commercial lineage piglets were weaned at 21-days-old and exhibited an average weight of 5.42 ± 0.55 kg.
The animals were allotted into 3 treatments groups (1: soybean oil, 2: palma oil and 3: blend of palma oil, dry skim milk and maltodextrin) following a randomized complete block design based on their initial body weight and sex with 10 replicates and 3 piglets per pen. The animals were housed in a nursery facility with a ceiling height of 3.5 m and lateral curtains and, suspended in metal pens with an area of 1.70 m2 and campanula heating. The pens had a partially slatted plastic flooring and a compact concrete floor under the campanula. There was a water layer under the pens that was replaced once or twice a week depending on the volume of waste produced.
Composition of experimental diets fed young pigs
Spray-dried blood meal
Dry skim milk
L-Lysine HCl (78.4 %)
DL-Methionine (99 %)
L-Threonine (98.5 %)
L-Tryptophan (98 %)
ZnO (73 %)
Chemical Composition (Calculated Values)
Metabolizable energy, kcal/kg
ADC = apparent digestibility coefficient,
%Cr2O3r = percentage of chromium oxide in the feed,
%Cr2O3f = percentage of chromium oxide in the feces,
%Nr = percentage of nutrients in the feed,
%Nf = percentage of nutrients in the feces
The feed marked with the chromium oxide was provided to piglets starting on the 7th day of the trial period, and the fecal samples were collected between day 11 and day 14 at 8:00 h and 16:00 h, identified and stored frozen (−20 °C) in plastic containers containing 1 kg of samples (multiple samples of three piglets per pen during the four-days collection period).
At the end of the experiment, samples of feed and feces from each pen were dehydrated in circulating air drying oven at 45 °C for 48 h and milled in a knives-type mill (0.595 mm). Subsequently, a mass of approximately 100.0 g of sample was sent to the laboratory for the analytical procedures.
Reagents and solutions
Ultrapure water (18.2 MΩ/cm) obtained from the PURELAB Ultra Ionic Elga system (Technology Drive Lowell, MA, USA), and spectroscopic grade nitric and hydrochloric acid (Darmstadt, Germany) were used. Stock solutions of the analytes and concomitants were prepared by dilution of Merck tritisol standards (Darmstadt, Germany). The remaining solutions including the extracting solutions were prepared from analytical grade reagents. All of the solutions were stored in polypropylene flasks.
All of the stock flasks of samples and standard solutions, glassware and accessories of the atomic absorption spectrometer (aspiration and spray system) and the UV–vis spectrophotometer were cleaned in nitric acid 10 % v/v for 24 h, then rinsed in ultrapure water and dried with jets of pure air before use.
Piglet feed and feces samples were cryogenically milled. To this end, a mass of approximately 1.00 g of sample was placed together with a magnetic bar into a polycarbonate jar, which was then closed and immersed in liquid nitrogen. The impact between the sample and the magnetic bar subjected to an oscillating magnetic field (20 impacts/s) pulverized the sample. The sample milling procedure consisted of an initial stage of 2 min of pre-freezing, 1 min for pulverization, and 1 min of freezing, followed by a second stage consisting of two cycles with two pulverization and freezing steps, for a total time of 8 min. This procedure produced particle size less than 60 μm [16, 17].
Extraction of the analytes
Acid mineralization (Ca, Mg and Cr)
After the drying and milling stage, part of the samples was mineralized in digestion blocks. To this end, masses of 100.0 mg of the cryogenically milled samples were transferred directly to 100 mL borosilicate glass tubes (Kjeldahl type), to which were added 3.0 mL of nitric acid 65 % m/m (HNO3 in pure form) was added at 100 °C initially. After complete oxidation of the organic matter, 1.0 mL of 70 % m/m perchloric acid (HClO4 in pure form) was added at 200 °C. The acid extracts obtained were diluted to 50 mL with ultrapure water.
Acid mineralization (P)
For the UV–vis analysis, samples were digested by acid mineralization in which 5.0 mL of nitric acid 65 % m/m (HNO3 in pure form) were poured to the homogenized sample (0.1 ± 0.0001 g) and it was left for 24 h into the Kjeldahl flasks. Afterwards, 2.0 mL of hydrogen peroxide 30 % m/m (H2O2 in pure form) were added to the mixture, heated for 60 min at 100 °C, and for additional 120 min at 200 °C, resulting in a clear solution which was transferred into a 50 mL volumetric flask and filled to the mark.
After the drying and milling stage, approximately 100.0 mg of sample and 20 mL of 0.10 mol/L HCl solution were transferred to 30 mL teflon flasks. The mixture was then subjected to ultrasonic shaking to extract the analytes. This procedure allowed the evaluation of different sonication times and ultrasonic powers in the analyte extraction process. The acid extracts obtained were separated from the remaining solid phase by centrifugation (3,500 rpm at 24 °C for 5 min).
Accuracy check for the UAE method
The quantification limit (QL) of the analytical curve was calculated in accordance to IUPAC procedures (QL = 10 sB/S, in which sB is the standard deviation of 20 consecutive measurements of the blank solution and S is the slope of the respective curves) . The method of quantification limit (MQL) was determined following to .
Application of the proposed method
Based on the values of the concentration of the nutrients and the percentage of chromium oxide determined in the samples under study, a calculation was made to estimate the apparent digestibility coefficients (ADC) of these nutrients permitting the comparison of both extraction methods.
The piglet (feed and feces) samples were dried in a MARCONI model MA-035 (Piracicaba, Brazil) circulating air drying oven, milled in a freezer cryogenic impact mill SPEX model 6750 (Metuchen, EUA) and mineralized in digestion blocks MARCONI model MA-447/6/100 (Piracicaba, Brazil).
An ultrasonic cell disrupter (horn-type ultrasonic probe) UNIQUE model USC-DC (Campinas, Brazil) equipped with a 3 mm titanium probe and operated at frequency of 20 kHz was used in the ultrasound-assisted extraction of the analytes. An ultracentrifuge model Bioagency Bio-Spin-320R (São Paulo, Brazil) was used to separate the ultrasound extracts from the remaining solid phase.
Calcium and magnesium were determined by using an atomic absorption spectrometer SHIMADZU AA-6800 (Tokyo, Japan) equipped with a background absorption correction with a deuterium lamp and self-reverse (SR) system. Phosphorus was measured using UV/Vis spectrophotometer Thermo Spectronic model Genesys 6 (Madison, EUA) equipped with a xenon lamp and an automatic six-cell changer with 1 cm quartz cells.
Standard mixed aqueous solutions of Ca, Mg and Cr were prepared from the dilution of Tritisol standards (Merck) containing 1,000 mg/L of the analytes in a medium of HCl 0.10 mol/L and 10 g/L of La3+ (used only for Ca and Mg standards). For each one, a blank was prepared containing all the components of the standard solutions except the analyte of interest.
For calibration, five standard solutions of phosphorus were prepared from the dilution of monopotassium phosphate (KH2PO4) 1.25 mmol/L and by the addition of 10.0 mL of the molybdate–vanadate reagent. The obtained solutions were left for 15 min to ensure the colour development and the absorbance signals were measured in acidic medium against the blank at λ = 420 nm with three replicates. The HCl solution 0.10 mol/L, after an addition of the molybdate–vanadate reagent was used as the blank for determination of phosphorus.
The optimal ranges of concentration of the analytical curves were as it follows: Ca: 0.5 to 4.0 mg/L; Mg: 0.1 to 0.5 mg/L; P: 1.55 to 9.29 mg/L and Cr: 1.0 to 8.0 mg/L. The equations for the calibration graphs obtained were as it follows: Ca: Abs = 0.05824 CCa + 0.001323, r = 0.9999; Mg: Abs = 1.4899 CMg + 0.03365, r = 0.9996; P: Abs = 0.0528 x CP + 0,00494, r = 0.9999 and, Cr: Abs = 0.0273 CCr + 0.0101, r = 0.9993.
The operational conditions employed in the determinations of Ca, Mg and Cr were:
Ca λ = 422.7 nm, spectra resolution: 0.5 nm, lamp current: 10 mA, flame stoichiometry: oxidizer, flame type: air-acetylene and gas flow 2.0 L/min; Mg λ = 285.2 nm, spectra resolution: 0.5 nm, lamp current: 10 mA, flame stoichiometry: oxidizer, flame type: air-acetylene and gas flow 1.8 L/min and Cr λ = 357.9 nm, spectra resolution: 0.5 nm, lamp current: 10 mA (low) and 400 mA (high), flame stoichiometry: slightly reducer, flame type: air-acetylene and gas flow 1.8 L/min.
The determination of chromium was necessary to calculate the chromium oxide content present in feed and feces for the subsequent estimation of the apparent digestibility coefficient of Ca, Mg and P. The absorbance signals were measured in the peak area, according to Welz (1992) .
The efficiency of the method in the analyte extraction was evaluated by varying the extraction time and ultrasonic power. This testing values were established by prior studies which utilized similar matrices and have found satisfactory extraction effects [21, 22]. The available time setting range was in 10 s interval up to 60 s. Tests at lower levels of time and powers of sonication than the minimum used here were not possible, due to operational specification settings.
Therefore, the minimum configurable time of the ultrasound digital timer started with 10 s. The sonication time testing was carried out varying the time scale by 10 units at time (10, 20, 30, 40 and 50 s) and evaluating the cycles of sonication time (1, 2, 3, 4 and 5 cycles). Initially, the tests were performed fixing the sonication time at 1 cycle of 10 s and varying the sonication power from 20 to 70 % and evaluating the absorbance signals of each analyte. Thereafter, the remaining combination were carried out (e.g. 1 cycle of 20 s and the evaluation from 20 to 70 % range of the sonication amplitude).
Concerning the levels of sonication power, the allowed power setting was from 20 to 90 % of total power operation (340 W). The range of 20 to 70 % of total power operation of the ultrasound was chosen because levels above 70 % caused an overheating of it, although the equipment was calibrated, and it was given proper maintenance.
The effect of the hydrochloric acid concentration on the process of extracting the analytes from the samples studied was also considered. The absorbance signals increased up to a concentration of 0.10 mol/L of acid, remaining constant in the 0.10 – 1.00 mol/L range. Thus, in all the remaining experiments, a 0.10 mol/L hydrochloric acid was used as extracting solution. The fixed volume adopted (20 mL) has been chosen due to prior literature results which achieved easily homogeneized liquid phase of the solution and lead to higher analyte extraction in acidic medium when ultrasonic probes were used [23–26].
All experimental data were analyzed in accordance with the general linear model procedure established by the Statistics Analysis System Institutes . The Tukey’s test was used to demonstrate statistical differences between groups. The pen of three pigs was considered to be the experimental unit for statistical analyses in this experiment. The variability of the data was expressed as the standard deviation and a probability level of (P < 0.05) was considered to be statistically significant.
Results and discussion
However, when the temperature of the extraction medium approaches the temperature of ebullition of the liquid phase, the sonication efficiency declines due to decreased surface tension of the medium, and increased vapor pressure inside the microbubbles, which causes a reduction in the shock waves . Thus, the sonication time of 5 cycles of 10 s achieved was considered effective in the elements extraction process and besides that this lower sonication time increased the sampling frequency, improving the analytical characteristics of the proposed method.
However, when very high vibrational amplitude is applied, a large number of cavitation bubbles are generated in the solution, which may be detrimental to the growth and collapse of these bubbles, and leads to a reduction of the energy released through the liquid phase [29–31]. The results indicated that the highest extraction efficiency was achieved on 102 W of the sonication power, because this power caused a lower increase in the temperature of the extracting solution.
The presence of an acidic medium is an essential requirement for quantitative extraction to be attained. For analytical techniques, hydrochloric acid is recommended and it was used in this study because it does not form volatile compounds with analytes, which are the origin of interferences and, in addition, it combined with the ultrasonic action promotes matrix oxidation so that analyte extraction is facilitated .
After optimizing the physicochemical parameters (sonication time and power), the accuracy and precision of the extraction method were tested by means of assay to recover the analytes from samples, which content were previously determined by acid mineralization.
Results obtained from the determinations of calcium, magnesium and phosphorus in standard material (NIST RM 8414)
Certified Values, g/kg
Determined Values, g/kg
0.145 ± 0.02
0.164 ± 0.01
0.145 ± 0.02
0.960 ± 0.09
1.023 ± 0.03
0.871 ± 0.01
0.840 ± 0.05
0.830 ± 0.02
0.820 ± 0.03
Apparent digestibility coefficient of nutrients in piglet pre-starter diets
55.23 ± 6.89
49.83 ± 10.13
51.61 ± 9.23
56.28 ± 5.69
51.92 ± 6.54
51.79 ± 7.18
30.56 ± 12.26
30.10 ± 7.30
21.99 ± 11.47
30.10 ± 8.28
29.14 ± 4.29
20.03 ± 7.83
64.82 ± 2.68a
63.14 ± 2.50a
68.16 ± 2.98a
45.34 ± 3.01b
55.90 ± 1.90b
46.52 ± 4.31b
The comparison of the values of ADC of phosphorus in the three feed presented in Table 3 proved to be higher than those calculated using acid mineralization (P < 0.05). Since pigs do not synthesize the endogenous phytase which promotes the hydrolysis of the ester linkage between phosphate group and inositol ring, releasing the phosphorus from the phytic acid molecule (inositol hexaphosphate) and considering that ultrasound-assisted extraction method does not mineralize this phytic molecule, the values of ADC calculated using this method were higher, might be due to Nfeces/Nfeed ratio only consider the phosphorus bioavailable fraction (i.e., fractions extracted in dihydrogen phosphate form). Thus, the ultrasound extraction allowed obtaining digestibility values of phosphorus more consistent to the real phosphorus absorption by piglets [9–13, 33].
The proposed method for determination of calcium, magnesium and phosphorus in piglet feed using ultrasound-assisted extraction allowed the calculation of the ADC of these nutrients and proved to be equivalent (P > 0.05) to those calculated using acid mineralization of the samples. In addition to this finding, the UAE validated method of Ca, Mg and P offered a QL of 53, 62 and 70 μg/L and a MQL of 10.6, 12.4 and 14 mg/kg respectively.
The efficiency of the extraction procedure based on the acoustic cavitation allowed the substitution of hazardous and high handling methods, as the nitric-perchloric acid digestion, by a green chemistry-type procedure. Furthermore, the toxic residues generated in acid extracts after acid mineralization of samples are not generated in the UAE process, thereby contributing to a clean chemistry-type procedure.
The speed of the process and reduced amount of sample required for the determination of these nutrients is useful in animal nutrition and analysis because it is a simple, fast, safe, precise and economical methodology.
The authors acknowledge the financial support of this work (grants: 2007/59778–0 and 2008/50986–1) provided by São Paulo Research Foundation (FAPESP).
- Lewis AJ, Southern LL. Swine Nutrition. 2nd ed. Boca Raton: CRC Press; 2001.Google Scholar
- Mavromichalis I. Applied nutrition for young pigs. Wallingford: CABI; 2006.Google Scholar
- Kane EA, Jacobson WC, Damewood PM. Use of radioactive chromium oxide in digestibility determinations. J Dairy Sci. 1959;42(8):1359–66.View ArticleGoogle Scholar
- Hanley F. The digestibility of foodstuffs and the effects of feeding selectivity on digestibility determinations in tilapia (Oreochromis niloticus). Aquaculture. 1987;66(2):163–79.View ArticleGoogle Scholar
- Kozloski GV, Flores EMM, Martins AF. Use of chromium oxide in digestibility studies: variation of the results as a function of the measurement method. J Sci Food Agric. 1998;76(3):373–6.View ArticleGoogle Scholar
- Silva FA, Neves RCF, Quintero-Pinto LG, Padilha CCF, Jorge SMA, Barros MM, et al. Determination of selenium by GFAAS in slurries of fish feces to estimate the bioavailability of this micronutrient in feed used in psiculture. Chemosphere. 2007;68(8):1542–7.PubMedView ArticleGoogle Scholar
- Mason TJ, Lorimer JP. Sonochemistry: Theory, Applications and Uses of Ultrasound in Chemistry. Chichester: Ellis Horwood; 1988.Google Scholar
- Elik A. Ultrasonic-assisted leaching of trace metals from sediments as a function of pH. Talanta. 2007;71(2):790–4.PubMedView ArticleGoogle Scholar
- Saha DC, Gilbreath RL. Analytical recovery of chromium from diet and feces determined by colorimetry and atomic absorption spectrophotometry. J Sci Food Agric. 1991;55(3):433–46.View ArticleGoogle Scholar
- Saha DC, Gilbreath RL. Faecal recovery and diurnal variation in excretion of dietary chromium by mature swine. J Sci Food Agric. 1991;56(4):407–18.View ArticleGoogle Scholar
- Bakker GCM, Jongbloed AW. The effect of housing system on apparent digestibility in pigs, using the classical and marker (chromic oxide, acid-insoluble ash) techniques, in relation to dietary composition. J Sci Food Agric. 1994;64(1):107–15.View ArticleGoogle Scholar
- Luque-García JL, Luque de Castro MD. Ultrasound: a powerful tool for leaching. Trends Anal Chem. 2003;22(1):41–7.View ArticleGoogle Scholar
- Hauptli L. Maltodextrina e óleos como fontes de energia para leitões, Ph. D. Thesis. Botucatu: Department of Animal Production, São Paulo State University; 2009.Google Scholar
- Rostagno HS, Teixeira A, Donzele JL, Gomes PC, Oliveira RFM, Lopes DC, et al. Brazilian Tables for Poultry and Swine: composition of feedstuffs and nutritional requirements. Minas Gerais: Universidade Federal de Viçosa; 2005.Google Scholar
- Shahat TM. Digestibility determination in catfish fingerling using internal and external markers. Vet Med J Giza. 1993;41(3):8391.Google Scholar
- Rosa CR, Moraes M, Neto JAG, Nóbrega JA, Nogueira ARA. Effect of modifiers on thermal behaviour of Se in acid digestates and slurries of vegetables by graphite atomic absorption spectrometry. Food Chem. 2002;79(4):517–23.View ArticleGoogle Scholar
- Neves RCF, Moraes PM, Saleh MAD, Loureiro VR, Silva FA, Barros MM, et al. FAAS determination of metal nutrients in fish feed after ultrasound extraction. Food Chem. 2009;113:679–83.View ArticleGoogle Scholar
- Currie LA. Nomenclature in evaluation of analytical methods including detection and quantification capabilities (IUPAC Recommendations 1995). Anal Chim Acta. 1999;391(2):105–26.View ArticleGoogle Scholar
- Manutsewee N, Aeungmaitrepirom W, Varanusupakul P, Imyim A. Determination of Cd, Cu and Zn in fish and mussel by AAS after ultrasound-assisted acid leaching extraction. Food Chem. 2007;101(2):817–24.View ArticleGoogle Scholar
- Welz B. Symbols and units for integrated absorbance in electrothermal atomic absorption spectrometry (ET-AAS). Spectrochim Acta B At Spectrosc. 1992;47(8):1043–4.View ArticleGoogle Scholar
- Capelo JL, Filgueiras AV, Lavilla I, Bendicho C. Solid–liquid extraction of copper from slurried samples using high intensity probe sonication for electrothermal atomic absorption spectrometry. Talanta. 1999;50(4):905–11.PubMedView ArticleGoogle Scholar
- Cal-Prieto MJ, Felipe-Sotelo M, Carlosena A, Andrade JM, López-Mahía P, Muniategui S, et al. Slurry sampling for direct analysis of solid materials by electrothermal atomic absorption spectrometry (ETAAS). A literature review from 1990 to 2000. Talanta. 2002;56:1–51.PubMedView ArticleGoogle Scholar
- Dolinsek F, Stupar J, Vrscaj V. Direct determination of cadmium and lead in geological and plant materials by electrothermal atomic absorption spectrometry. J Anal At Spectrom. 1991;6:653–60.View ArticleGoogle Scholar
- Mierzwa J, Dobrowolski R. Silica gel analysis by slurry-sampling graphite-furnace atomic absorption spectrometry. Fresenius J Anal Chem. 1994;348(7):422–5.View ArticleGoogle Scholar
- Mierzwa J, Dhindsa HS. Determination of arsenic, chromium, and nickel in slurried talc samples by electrothermal AAS. At Spectrosc. 1998;19(1):6–9.Google Scholar
- Saleh MAD, Berto DA, Padilha PM. Ultrasound-assisted extraction of Na and K from swine feed and its application in a digestibility assay: A green analytical procedure. Ultrason Sonochem. 2013;20:1353–8.PubMedView ArticleGoogle Scholar
- SAS Institute Inc. SAS OnlineDoc®, version 8, Cary. 1999.Google Scholar
- Filgueiras AV, Capelo JL, Lavilla I, Bendicho C. Comparison of ultrasound-assisted extraction and microwave-assisted digestion for determination of magnesium, manganese and zinc in plant samples by flame atomic absorption spectrometry. Talanta. 2000;53(2):433–41.PubMedView ArticleGoogle Scholar
- Elik A. Ultrasound assisted pseudo-digestion of street dust samples prior to determination by atomic absorption spectrometry. Talanta. 2005;66:882–8.PubMedView ArticleGoogle Scholar
- Nascentes CC, Korn M, Arruda MAZ. A fast ultrasound-assisted extraction of Ca, Mg, Mn and Zn from vegetables. Microchem J. 2001;69(1):37–43.View ArticleGoogle Scholar
- Ruiz-Jiménez J, Luque-Garcia JL, Luque de Castro MD. Dynamic ultrasound-assisted extraction of cadmium and lead from plants prior to electrothermal atomic absorption spectrometry. Anal Chim Acta. 2003;480(2):231–7.View ArticleGoogle Scholar
- Bermejo-Barrera P, Moreda-Piñeiro A, Moreda-Piñeiro J, Bermejo-Barrera A. Comparative study on the use of Ir, W and Zr-coated graphite tubes for the determination of chromium in slurries of human scalp hair by electrothermal atomic absorption spectrometry. Fresenius J Anal Chem. 1998;360(6):712–6.View ArticleGoogle Scholar
- Moraes PM, Loureiro VR, Padilha PM, Neves RCF, Saleh MAD, Santos FA, et al. Determinação de fósforo biodisponível em rações de peixes utilizando extração assistida por ultra-som e espectrofotometria no visível. Quim Nova. 2009;32(4):923–7.View ArticleGoogle Scholar
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