- Open Access
Glutamine and glutamate supplementation raise milk glutamine concentrations in lactating gilts
© Manso et al; licensee BioMed Central Ltd. 2012
- Received: 6 January 2012
- Accepted: 28 February 2012
- Published: 28 February 2012
Glutamine is the most abundant amino acid in milk, and lactation is associated with increased glutamine utilization both for milk synthesis and as a fuel for the enlarged small intestine. A number of recent studies have indicated that lactation is accompanied by a mild catabolic state in which skeletal muscle proteins are degraded to provide amino acids that are used to synthesize additional glutamine. In this study we tested the hypothesis that supplemental L-glutamine or the commercially available glutamine supplement Aminogut (2.5% by weight mixed into daily feed) provided to gilts from 30 days prior to parturition until 21 days post-parturition would prevent a decrease in skeletal muscle glutamine while increasing the glutamine content of the milk. Muscle glutamine content decreased (P < 0.05) in control animals during lactation but this was prevented by supplementation with either L-glutamine or Aminogut. In this study, neither lactation nor supplementation had any effect on plasma glutamine or glutamate content. Free glutamine, and the total glutamine plus glutamate concentrations in milk from the control and the Aminogut group rose (P < 0.05) during the first 7 days of lactation, with milk concentrations in the L-glutamine supplemented group showing a similar trend (P = 0.053). Milk glutamate remained constant between day 7 and 21 of lactation in the control and L-glutamine supplemented groups, but by day 21 of lactation the free glutamine, glutamate, and glutamine plus glutamate concentrations in milk from Aminogut-treated gilts were higher than those of control gilts. Thus dietary glutamine supplementation can alleviate the fall in intramuscular glutamine content during lactation in gilts, and may alleviate some of the catabolic effects of lactation. Furthermore, the increased milk glutamine content in the supplemented gilts may provide optimum nutrition for piglet development.
- skeletal muscle
Glutamine is the most abundant free -amino acid in the body of most mammals and the majority of stored glutamine is concentrated in skeletal muscle [1, 2]. Glutamine and glutamate comprise between 5 and 15% of the amino acid content of most foods and commercial feedstuffs, but since these amino acids are effectively metabolized by the epithelial cells of the small intestine there is little net absorption at normal dietary intake levels . Thus, the large glutamine pool in the body arises from de novo synthesis in the skeletal muscle through the action of glutamine synthetase. Catabolic stress increases the need for glutamine by a number of tissues. This demand is met by increased glutamine release from skeletal muscle. Initially, release of glutamine from the existing intramuscular pool results in a drop in the intracellular glutamine concentration. However, continued release of glutamine requires increased synthesis. An increase in net proteolysis within the muscle cells provides the amino acid substrates for glutamine synthesis [3, 4]. We recently determined that, in the horse, lactation represents a mild catabolic state accompanied by a loss of lean body mass and a decrease in muscle glutamine content . Additional evidence supports a similar conclusion for a number of other species, including the pig [6–11].
It is well established that during lactation there is increased glutamine utilization since glutamine is the most abundant amino acid in the milk of most species [12–17]. Additionally, enlargement of the intestines during pregnancy and lactation also increases the glutamine requirements of the intestinal epithelial cells . The source of the substrates for increased glutamine synthesis to meet these demands has not been definitively identified, but it is generally thought that amino acids obtained from increased dietary protein intake would suffice . However, our work with the horse suggests that, in addition to dietary amino acids, muscle proteolysis also provides some of the substrates for glutamine synthesis during lactation . Therefore, we hypothesized that supplemental dietary glutamine (and/or glutamate) could provide the extra glutamine required for milk production, thereby limiting the need to utilize endogenous proteins and aiding in the maintenance of lean body mass. In this study we tested this hypothesis in gilts and, in addition, we investigated whether dietary glutamine supplementation can raise milk glutamine concentrations.
The nutritional composition of the commercial feeds fed to the pregnant and lactating gilts.
Main ingredients, kg
Extruded full-fat soybeans
Trace mineral premix
Crude protein, %
Crude fiber, %
Total phosphorus, %
Metabolizable energy, kcal/kg
From 30 days before until 21 days after farrowing the gilts were divided into three groups, which received dietary supplementation as follows: control group, no supplementation; glutamine group, L-glutamine supplementation (2.5% by weight mixed into the daily ration); Aminogut group, Aminogut supplementation (2.5% by weight mixed into the daily feed). Aminogut is a commercially available dietary supplement produced by Ajinomoto do Brazil (São Paulo, Brazil) that contains both free glutamine (min 10%) and glutamic acid (min 10%). On the day of farrowing all litters were reduced to 10 or 11 piglets; however, since the work was carried out on a commercial farm it was only possible to determine the body weight of the piglets on the day of birth and at weaning. All animal work was approved by CEUA - Ethical Committee of Animal Utilization/UFRPE (Federal Rural University of Pernambuco).
Blood and muscle samples were taken 30 days prior to parturition, at parturition and on days 7 and 21 of lactation; milk samples were taken at parturition and on days 7 and 21 of lactation. All sampling was done 2 to 3 hours after the morning feed. Blood samples were drawn from an auricular vein and placed on ice. Muscle samples were obtained by biopsy of the superficial gluteus muscle. Briefly, a small incision was made in the skin and adipose tissue over the superficial gluteus muscle to allow the introduction of the biopsy needle and the muscle was sampled at a depth of approximately 8 cm below the skin (well beyond the fat layer). After collection, muscle samples were stored at -80°C until analysis. Only the control animals underwent the initial muscle biopsy at 30 days before parturition. To facilitate milk collection the gilts received an injection of oxytocin (0.5 mL, i.v.; Prolacton 1:10000, Tortuga, Brazil). Milk was collected from the abdominal teats and immediately placed on ice.
Blood and milk samples were deproteinized by the addition of an equal volume of perchloric acid (10% w/v), and muscle samples (100 to 200 mg) were homogenized in 4 to 5 volumes of perchloric acid (10% w/v). The acid extracts were then centrifuged (5,000 g for 15 minutes) to remove proteins and the supernatants neutralized with potassium hydroxide and stored at -80°C until analysis.
Glutamine and glutamate determination
Glutamine was hydrolyzed to glutamate by glutaminase. Glutamate levels were then determined enzymatically using glutamate dehydrogenase and the reduction of NAD+ .
Gilts that were unable to maintain 10 to 11 piglets were excluded from the study. The number of gilts that completed the study and were included in the statistical analysis were 6, 5 and 8 in the control, glutamine, and Aminogut groups, respectively. All results were analyzed by ANOVA using SAS Statistical Package version 9.2 (SAS Institute, Cary, NC). Dunnett's test was used to compare body weight, back fat thickness, and glutamine/glutamate in blood and muscle during lactation to the pre-farrowing value. Tukey's post hoc testing was used to compare differences between individual groups (time and treatment) in milk concentrations. Differences were considered statistically significant if P < 0.05.
Body weight and back fat thickness in gilts
(n = 6)
(n = 5)
(n = 8)
Body weight, kg
166.6 ± 8.1
185.1 ± 9.1
175.2 ± 6.5
182.7 ± 6.2
185.6 ± 9.5
174.5 ± 9.8
176.3 ± 5.2
181.7 ± 9.4
162.0 ± 5.2
165.0 ± 5.2
157.5 ± 9.7
147.8 ± 7.4*
17.2 ± 0.9
18.4 ± 1.8
15.3 ± 0.4
16.5 ± 0.5
23.6 ± 2.9
16.1 ± 0.8
15.3 ± 1.6
19.8 ± 2.5
14.0 ± 0.6
13.7 ± 1.9
13.4 ± 1.9*
11.3 ± 0.4*
Litter size and piglet body weight at birth and weaning (21 days)
(n = 6)
(n = 5)
(n = 8)
Body weight, g
1.59 ± 0.04
1.39 ± 0.17
1.27 ± 0.07
5.92 ± 0.27
6.27 ± 0.63
5.67 ± 0.29
Number of piglets
Muscle glutamine and glutamate
Blood glutamine and glutamate
Milk glutamine and glutamate
Traditionally glutamine is not considered an essential amino acid. The body is able to synthesize considerable quantities of glutamine (and glutamate), and in healthy adult mammals there is no evidence of any glutamine shortage [1, 2]. However, during times of severe trauma or infection (hypercatabolic states) there is an increase in the glutamine requirement of a number of tissues, including the immune cells, the liver and the kidneys. Increased glutamine is then supplied by de novo synthesis from amino acids derived from the breakdown of skeletal muscle protein and an increase in the activity of skeletal muscle glutamine synthetase. Despite this increase in glutamine synthesis and release by the muscle, there is considerable evidence that under conditions of severe stress endogenous glutamine production is insufficient to meet the body's needs. A number of clinical trials have shown benefits of both enteral and parenteral glutamine supplementation, with successful trials involving large (up to and above 50 g glutamine per day per person) amounts of glutamine supplementation [3, 4]. There is little evidence, however, of any beneficial effects of glutamine supplementation in healthy individuals .
In our work with lactating horses we discovered that lactation was accompanied by a decrease in glutamine pools and a loss of lean body mass . This led us to conclude that lactation was a mildly catabolic state in this specie, and additional studies have reported that lactation represents a mild, transient catabolic state in a number of other species including the pig [6–11]. While it is well recognized that glutamine needs are greatly increased during lactation, both to provide milk glutamine and to fuel the enlarged small intestine, it has usually been supposed that additional glutamine to meet this demand would simply be synthesized from the extra dietary amino acids consumed during lactation . Our findings in the horse suggest that dietary amino acids are insufficient to meet this increased demand, and that the mare draws additional substrates from her own lean mass to provide the glutamine required by other tissues. In our current work, lower glutamine/glutamate concentrations in skeletal muscle early (day 7) in lactation in gilts receiving the control diet also indicate that a mild catabolic state is present in early lactation. That supplemental glutamine/glutamate prevents such changes during lactation indicates that perhaps lean body mass may be maintained during lactation with appropriate dietary intervention.
In addition to sparing the lean body mass of the sow, supplemental glutamine could also result in an increase in milk glutamine. Since glutamine is readily hydrolyzed to glutamate and is thus relatively labile during storage, consideration of glutamine plus glutamate together in stored samples might best reflect changes in the glutamine pool in vivo. In addition, the combined concentrations of glutamine and glutamate are additionally relevant since the pig intestine metabolizes both dietary glutamine and glutamate similarly.
The high glutamine content in the milk of most species is likely due to the high glutamine needs associated with rapid growth and cell division in neonatal tissues, particularly in the neonatal small intestine and gut-associated lymphoid tissue (GALT). It is well established that the neonatal gut is particularly sensitive to stress, and that abrupt early weaning is often associated with negative growth and pathological outcomes that are clearly related to intestinal dysfunction. A number of studies over the past 15 years have shown that glutamine and/or glutamate supplementation can be beneficial to piglet gut health and weight gain [15, 21–27]. Most of these studies began at weaning, but at least one addressed the question of supplemental glutamine during the suckling period. Wu and colleagues  gave suckling piglets glutamine (3.42 mmol/kg) twice daily by oral gavage and found improvements in intestinal function and growth performance. Such findings strongly support the idea that maternal milk does not usually contain optimal amounts of glutamine (and/or glutamate) and that provision of exogenous glutamine is beneficial to suckling animals. For most species it is not practical to supply supplemental amino acids by gavage, but our results indicate that supplementation of the sow may offer an alternative means of increasing glutamine delivery to the piglet. The concentration of glutamine/glutamate in milk from gilts receiving supplemental glutamine and glutamate (particularly in the Aminogut group) was double that of the control animals. Thus, piglets suckling from supplemented mothers received more dietary glutamine and glutamate, and any potential associated benefits would also likely be received. In this study, supplemental glutamine or Aminogut did not change litter size or piglet weight at birth or weaning. A similar study that supplemented the diet of lactating sows with 2.5% glutamine also showed higher milk glutamine concentrations at days 7 and 21 of lactation with no differences in litter parameters except for some small effects on piglet intestinal anatomy . Our study was conducted on a commercial pig farm, therefore it was not possible to sample the piglets or to follow them in the immediate post-weaning period. Thus further research is needed to quantify the benefits of providing extra glutamine to the piglets.
During lactation, increased glutamine utilization requires an increase in endogenous glutamine production. In skeletal muscle, the branched chain amino acids that are the substrates for increased glutamine production are derived from dietary protein or from intracellular proteolysis. Our work, together with numerous other studies, indicates that lactation is accompanied by a loss of lean body mass that is, in part, related to an increase in muscle glutamine synthesis. The finding that skeletal muscle glutamine content is maintained in animals receiving glutamine/glutamate supplementation indicates that suitable dietary interventions could ameliorate some of the negative effects of lactation in gilts. Furthermore, the increased glutamine and glutamate in the milk of the supplemented gilts may improve the growth and intestinal health of their piglets throughout the suckling, weaning and post-weaning periods.
Financial Support: Ajinomoto do Brasil/FUNCAP - Fundação Cearense de Apoio ao Desenvolvimento Científico/CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico.
- Curthoys NP, Watford M: Regulation of glutaminase activity and glutamine metabolism. Annu Rev Nutr. 1995, 15: 133-159. 10.1146/annurev.nu.15.070195.001025.View ArticlePubMedGoogle Scholar
- Watford M: Glutamine metabolism and function in relation to proline synthesis and the safety of glutamine and proline supplementation. J Nutr. 2008, 138: 2003S-2007S.PubMedGoogle Scholar
- Tjader I, Berg A, Wernerman J: Exogenous glutamine - compensating a shortage? Crit. Care Med. 2007, 35: S553-S556. 10.1097/01.CCM.0000278602.41352.0E.View ArticleGoogle Scholar
- Wernerman J: Clinical use of glutamine supplementation. J Nutr. 2008, 138: 2040S-2044S.PubMedGoogle Scholar
- Manso Filho HC, McKeever KH, Gordon ME, Costa HEC, Lagakos WS, Watford M: Changes in glutamine metabolism indicate a mild catabolic state in the transition mare. J Anim Sci. 2008, 86: 3424-3431.View ArticlePubMedGoogle Scholar
- Clowes EJ, Williams IH, Baracos VE, Pluske JR, Cegielski AC, Zak LJ, Aherne FX: Feeding lactating primiparous sows to establish three divergent metabolic states: II. Effect on nitrogen partitioning and skeletal muscle composition. J Anim Sci. 1998, 76: 1154-1164.PubMedGoogle Scholar
- Clowes EJ, Aherne FX, Baracos VE: Skeletal muscle protein mobilization during the progression of lactation. Am J Physiol Endocrinol Metab. 2005, 288: E564-E572.View ArticlePubMedGoogle Scholar
- Kim SW, Easter RA: Nutrient mobilization from body tissues as influenced by litter size in lactating sows. J Anim Sc. 2001, 79: 2179-2186.Google Scholar
- Meijer GAL, van der Meulen J, van Vuuren AM: Glutamine is a potentially limiting amino acid for milk production in dairy cows: a hypothesis. Metabolism. 1993, 42: 358-364. 10.1016/0026-0495(93)90087-5.View ArticlePubMedGoogle Scholar
- Meijer GAL, van der Meulen J, Bakker JGM, van der Koelen CJ, van Vuuren AM: Free amino acids in plasma and muscle of high yielding dairy cows in early lactation. J Dairy Sci. 1995, 78: 1131-1141. 10.3168/jds.S0022-0302(95)76730-3.View ArticlePubMedGoogle Scholar
- Pine AP, Jessop NS, Allan GF, Oldham JD: Maternal protein reserves and their influence on lactational performance in rats. 4. Tissue protein synthesis and turnover associated with mobilization of maternal protein. Brit J Nutr. 1994, 72: 831-844. 10.1079/BJN19940088.View ArticlePubMedGoogle Scholar
- Agostoni C, Carrato B, Boniglia C, Lammardo AM, Riva E, Sanzini E: Free glutamine and glutamic acid increase in human milk through a three-month lactation period. J Pediar Gastroenterol Nutr. 2000, 31: 508-512. 10.1097/00005176-200011000-00011.View ArticleGoogle Scholar
- Carratu B, Boniglia C, Scalise F, Ambruzzi AM, Sanzini E: Nitrogenous compounds of human milk: non-protein nitrogen, true protein and free amino acids. Food Chem. 2003, 81: 357-362. 10.1016/S0308-8146(02)00430-2.View ArticleGoogle Scholar
- Davis TA, Nguyen HV, Garcia-Bravo R, Fiorotto ML, Jackson EM, Reeds PJ: Amino acid composition of the milk of some mammalian species changes with stage of lactation. Brit J Nutr. 1994, 72: 845-853. 10.1079/BJN19940089.View ArticlePubMedGoogle Scholar
- Haynes TE, Li P, Li X, Shimotori K, Sato H, Flynn NE, Wang J, . Knabe DA, Wu G: L-Glutamine or L-alanyl-L-glutamine prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Amino Acids. 2009, 37: 131-142. 10.1007/s00726-009-0243-x.View ArticlePubMedGoogle Scholar
- Sarwar G, Botting HG, Davis TA, Darling P, Pencharz PB: Free amino acids in milks of human subjects and other primates and non-primates. Brit J Nutr. 1998, 79: 129-131. 10.1079/BJN19980023.View ArticlePubMedGoogle Scholar
- Wu G, Knabe DA: Free and protein-bound amino acids in sow's colostrom and milk. J Nutr. 1994, 124: 415-424.PubMedGoogle Scholar
- Watford M, Erbelding EJ, Smith EM: Glutamine utilization in rat small intestine: response to lactation. Biochem Soc Trans. 1986, 14: 1058-1059.View ArticleGoogle Scholar
- Lund P: L-Glutamine determination with glutaminase and glutamate dehydrogenase. Methods of Enzymatic Analysis. Edited by: Bergmeyer H. 1974, Academic Press, New York, NY, 4: 1719-1722.Google Scholar
- Gleeson M: Dosing and efficacy of glutamine supplementation in human exercise and sport training. J Nutr. 2008, 138: 2045S-2049S.PubMedGoogle Scholar
- Kitt SJ, Miller PS, Lewis AJ, Fischer RL: Effects of glutamine on growth performance and small intestine villus height in weanling pigs. 2002 Nebraska Swine Report. 2002, 29-32.Google Scholar
- Kitt SJ, Miller PS, Fischer RL: Effects of sow dietary glutamine supplementation on sow and litter performance, subsequent weanling pig performance and intestinal development after an immune challenge. 2004 Nebraska Swine Report. 2004, 14-17.Google Scholar
- Lopez-Pedrosa JM, Manzano M, Baxter JH, Rueda R: N-Acetyl-L-Glutamine, a liquid-stable source of glutamine, partially prevents changes in body weight and on intestinal immunity induced by protein energy malnutrition in pigs. Dig Dis Sci. 2007, 52: 650-658. 10.1007/s10620-006-9500-y.View ArticlePubMedGoogle Scholar
- Wang J, Chen L, Li P, Li X, Zhou H, Wang F, Li D, Yin Y, Wu G: Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J Nutr. 2008, 138: 1025-1032.View ArticlePubMedGoogle Scholar
- Wu G, Meier SA, Knabe DA: Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs. J Nutr. 1996, 126: 2578-2584.PubMedGoogle Scholar
- Yoo SS, Field CJ, McBurney MI: Glutamine supplementation maintains intramuscular glutamine concentrations and normalizes lymphocyte function in infected early weaned pigs. J Nutr. 1997, 127: 2253-2259.PubMedGoogle Scholar
- Yi GF, Carroll JA, Allee GL, Gaines AM, Kendall DC, Usry JL, Toride Y, Izuru S: Effect of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses of Escherichia coli K88 ± challenged weaned pigs. J Animal Sci. 2005, 83: 634-643.Google Scholar
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