Fig. 7

CircRALGPS2-212aa protein interacts with PARP1 protein. A Proteins identified by co-immunoprecipitation with FLAG antibody in granulosa cells. B and C KEGG enrichment analysis of identified proteins. D The protein abundance and gray value statistics of PARP1 and HMGB1 after transfection with ov-circRALGPS2-FLAG plasmid. E The protein abundance and gray value statistics of PARP1 and HMGB1 after transfection with pcDNA3.1-circRALGPS2-FLAG plasmid. F Western blot results of PARP1 antibody after granulosa cells were co-immunoprecipitated with FLAG antibody. G Western blot results of HMGB1 antibody after granulosa cells were co-immunoprecipitated with FLAG antibody. H Western blot results of PARP1 antibody after granulosa cells were co-immunoprecipitated with PARP1 antibody. I Western blot results of HMGB1 antibody after granulosa cells were co-immunoprecipitated with PARP1 antibody. ^*P < 0.05, ^**P < 0.01. Data are presented as mean ± SEM. n = 3 for Western blotting