Betaine addition to the diet alleviates intestinal injury in growing rabbits during the summer heat through the AAT/mTOR pathway

Table 2 Primer sequences for quantitative real-time polymerase chain reaction

Gene	Primer sequences (5′→3′)	Product length, bp	Accession number
β-action	F: CAGAAACGAGACGAGATTGGC R: CAATCAAAGTCCTCGGCCACA	189	NM_001101683.1
IL-2	F: TGCCCAAGAAGGTCACAGAA R: CCCCCATGAGAGTTTTTGCC	106	NM_001163180.1
IL-4	F: GCGACATCATCCTACCCGAA R: TCGGTTGTGTTCTTGGGGAC	115	NM_001163177.1
mTOR	F: CTTGAGTCCGAAGAGGATGG R: ATGAGGGTGGCAAGAAAGTG	187	XM_008262137.2
P70S6k1	F: TGTGACGACAAGTGGGGAAG R: TGGCCGTTTGGAGATCATGG	100	NM_001101690.1
SLC6A20	F: GGATGGCTGACTCAAGAACC R: CACACACTGAGGCGTAGAGC	226	XM_008269584.2
SLC7A6	F: TGCCCCTCTTCAGTGATACC R: GCAAAGCTTCTGGGTGACTC	161	XM_017342318.1
SLC9A3	F: ATCGGCTCATTGAGGTGAAC R: TCCCATTGGTGATTGGTGAT	198	NM_001082107.1
SLC36A1	F: ACTACTCGGAGGGCATGAGT R: TGAAGATGGGAGCGTTGCTT	135	XM_008254846.2
SLC38A2	F: TGTGCAGCCAAAGATTTCAG R: GACGGGAGGATGAAGATCAA	158	XM_002712695.3
TNF-α	F: AGCCCACGTAGTAGCAAACC R: TGAGTGAGGAGCACGTAGGA	192	XM_008262537.2
4EBP-2	F: GTTGGATCGTCGCAATTCTCC R: ATTAAGGTGCCAGGGCTAGTG	87	XM_002718436.3

IL-2 Interleukin-2, IL-4 Interleukin-4, mTOR Mammalian target of rapamycin, P70S6k1 P70 ribosomal protein S6 kinase 1, SLC7A6 Solute carrier family 7 member 6, SLC9A3 Solute carrier family 9 member 3, SLC6A20 Solute carrier family 6 member 20, SLC36A1 Solute carrier family 36 member 1, SLC38A2 Solute carrier family 38 member 2, TNF-α Tumor necrosis factor-alpha, 4EBP-2 Eukaryotic initiation factor 4E-binding protein-2

ISSN: 2049-1891