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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Coated sodium butyrate ameliorates high-energy and low-protein diet induced hepatic dysfunction via modulating mitochondrial dynamics, autophagy and apoptosis in laying hens

Fig. 7

CSB triggered autophagy in HELP-induced laying hens. A Representative TEM images showing autophagosome formation (red arrows, autophagosomes; n = 6). B The mRNA levels of autophagy-related genes (n = 6). C Representative LC3B (green) and p62 (red) immunofluorescence staining images in hepatic tissue (n = 6). Nuclei were stained with DAPI (blue). D LC3B and p62 relative fluorescence intensity (n = 6). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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