Table 2 Primer sequences of the target genes
Gene | Primer sequence (5´→3´) | Product length, bp | GenBank accession |
|---|---|---|---|
SGLT1 | F: GCAACAGCAAAGAGGAGCGTAT | 137 | NM_001164021.1 |
R: GCCACAAAACAGGTCATAGGTC | |||
GLUT2 | F: GACACGTTTTGGGTGTTCCG | 149 | NM_001097417.1 |
R: GAGGCTAGCAGATGCCGTAG | |||
PepT1 | F: GCCAAAGTCGTCAAGTGC | 100 | NM_214347 |
R: GGTCAAACAAAGCCCAGA | |||
SLC7A1 | F: TCTTTGCAGGTCGTTTGGGA | 137 | NM_001012613.1 |
R: GGCTGATCACCTGTTGGAGT | |||
TNF-α | F: CGTGAAGCTGAAAGACAACCAG R: GATGGTGTGAGTGAGGAAAACG | 121 | NM_214022.1 |
IL-1β | F: CAGCTGCAAATCTCTCACCA R: TCTTCATCGGCTTCTCCACT | 112 | NM_214055.1 |
IL-6 | F: TTCACCTCTCCGGACAAAAC R: TCTGCCAGTACCTCCTTGCT | 122 | NM_001252429.1 |
IL-8 | F: AGTTTTCCTGCTTTCTGCAGCT R: TGGCATCGAAGTTCTGCACT | 72 | NM_213867.1 |
IL-10 | F: CCTGGAAGACGTAATGCCGA R: CACGGCCTTGCTCTTGTTTT | 148 | NM_214041.1 |
RIG-I | F: AGAGCAGCGGCGGAATC | 82 | NM_213804.2 |
R: GGCCATGTAGCTCAGGATGAA | |||
MDA5 | F: TCCGGGAAACAGGCAACTC | 75 | NM_001100194.1 |
R: CAAAGGATGGAGAGGGCAAGT | |||
MAVS | F: TGGGTACAGTCCTTCATCGG R: GGGTAACTTGGCTCATCCTCT | 116 | NM_020746.5 |
TLR2 | F: TCACTTGTCTAACTTATCATCCTCTTG | 162 | XM_005653576.3 |
R: TCAGCGAAGGTGTCATTATTGC | |||
TLR3 | F: TGGAAAAAGGAATGGCCAGC R: ACAAGGCAAACTCCTGCTCA | 265 | NM_001042467.3 |
TLR4 | F: TTACAGAAGCTGGTTGCCGT R: TCCAGGTTGGGCAGGTTAGA | 152 | NM-001293316.1 |
TLR7 | F: CAATGGTCCCTGAGCGTTTG R: AGCCTGGTTGAAGACAGCAG | 126 | NM_016562 |
TLR9 | F: CACGACAGCCGAATAGCAC | 121 | NM_213958.1 |
R: GGGAACAGGGAGCAGAGC | |||
MyD88 | F: GTGCCGTCGGATGGTAGTG | 65 | NM001099923 |
R: TCTGGAAGTCACATTCCTTGCTT | |||
TRIF | F: CAAGTGGAGGAAGGAACAGG R: CAACTGCGTCTGGTAGGACA | 139 | XM_003362039.1 |
TRAF6 | F: CAAGAGAATACCCAGTCGCACA R: ATCCGAGACAAAGGGGAAGAA | 122 | NM-001105286.1 |
β-actin | F: GGATGACGATATTGCTGCGC R: GATGCCTCTCTTGCTCTGGG | 190 | XM_003124280.5 |