Table 2 Primer sequences for qRT-PCR
Gene | Primer Sequence (5´→3´) | Size, bp | Accession No. |
|---|---|---|---|
ZO-1 | Forward: ACGCTGGTGAAATCAAGGAAGAA Reverse: AGGGACATTCAACAGCGTGGC | 255 | XM_013093747.1 |
ZO-2 | Forward: ACAGTGAAAGAAGCTGGCGTAG Reverse: GCTGTATTCCCTGCTACGGTC | 131 | XM_005019888.2 |
OCLN | Forward: CAGGATGTGGCAGAGGAATACAA Reverse: CCTTGTCGTAGTCGCTCACCAT | 160 | XM 013109403.1 |
CLDN-1 | Forward: TCATGGTATGGCAACAGAGTGG Reverse: CGGGTGGGTGGATAGGAAGT | 179 | XM_013108556.1 |
CLDN-2 | Forward: CTCCTCCTTGTTCACCCTCATC Reverse: GAACTCGCTCTTGGGTTTGTG | 160 | XM_005009661.2 |
MUC2 | Forward: GGGCGCTCAATTCAACATAAGTA Reverse: TAAACTGATGGCTTCTTATGCGG | 150 | XM_005024513.2 |
Notch1 | Forward: GTGAAATCGATGCGGACTGC Reverse: ATGAAGTCGGAGATGACGGC | 148 | XM_038164890.1 |
Notch2 | Forward: CGCATCCGTGCTTGAACAAA Reverse: AGGGAGACCTGCTGCATAGA | 123 | XM_038182661.1 |
IL-8 | Forward: GATTTCCGTGGCTCTGTCCC Reverse: CTCTGCGTCAGCTTCACATC | 129 | NM_001310420.1 |
IL-22 | Forward: CTAAAATGGCCAGGGCCTCA Reverse: CGCCACCTCCTCAGTGTATG | 179 | XM_038167538.1 |
TNF-α | Forward: ACAGGACAGCCTATGCCAAC Reverse: ACAGGAAGGGCAACACATCT | 165 | XM_005019359.2 |
IgA | Forward: TCGCTCAAGGAACCCATCGT Reverse: GCGGGACCACGAGAACTTCA | 174 | U27222.1 |
TLR4 | Forward: ACCCATTGTCACCAACATCATC Reverse: TGCCTCAGCAAGGTCTTATTCA | 195 | JN048668.1 |
MYD88 | Forward: GGAGGATGGTGGTCGTCATT Reverse: CCGCAGGATACTTGGGAACT | 158 | NM_001310832.1 |
NF-κB | Forward: GCTGGCTAATTGGACCGACA Reverse: CAGGTCTGGCACGTATCTCG | 122 | XM_021271051.2 |
β-actin | Forward: AGAAATTGTGCGTGACATCAA Reverse: GGACTCCATACCCAAGAAAGAT | 227 | XM013108556.1 |