Effect of organic mineral supplementation in reducing oxidative stress in Holstein calves during short-term heat stress and recovery conditions

Table 6 Plasma SOD, GPX, MDA, and HSPs of Holstein calves supplemented with inorganic and organic minerals at different temperatures

Parameters	Heat				Recovery				SEM⁵	Mixed P value⁶
Parameters	Con¹	IM²	OM³	HOM⁴	Con¹	IM²	OM³	HOM⁴	SEM⁵	Temperature	Minerals	T × M
SOD, U/mL	33.38	35.72	33.78	35.69	32.03	33.05	33.17	34.08	0.990	0.115	0.368	0.892
GPX activity, nmol/min/mL	34.70	35.98	36.72	36.21	34.14	36.05	36.21	37.41	3.469	0.984	0.920	0.994
MDA, μmol/L	1.53	1.50	1.37	1.24	1.60	1.59	1.44	1.29	0.144	0.549	0.263	0.999
HSP27kDa, pg/mL	742.57	691.42	663.04	667.63	833.49	806.98	715.89	753.28	60.783	0.077	0.451	0.972
HSP70kDa, ng/mL	0.24	0.22	0.22	0.21	0.25	0.22	0.20	0.20	0.017	0.701	0.169	0.796
HSP90kDa, ng/mL	6.41	5.90	5.98	5.58	6.14	5.28	5.89	5.38	0.678	0.542	0.620	0.983

SOD Superoxide dismutase, GPX Glutathione peroxidase, MDA Malondialdehyde, HSP Heat shock protein
¹Con: Basal diet (without mineral supplementation)
²IM: Basal diet + inorganic minerals supplementation
³OM: Basal diet + organic minerals supplementation
⁴HOM: Basal diet + higher organic minerals supplementation
⁵Standard error of means
⁶P value obtained from the mixed procedure of SAS with temperature effect (B), mineral effect (M), and interaction effect between temperature and minerals (T × M). A P value less than 0.05 indicates that the results are statistically significant

ISSN: 2049-1891