Effect of organic mineral supplementation in reducing oxidative stress in Holstein calves during short-term heat stress and recovery conditions

Table 5 Serum biochemistry and mineral concentrations of Holstein calves supplemented with inorganic and organic minerals at different temperatures

Parameters	Heat				Recovery				SEM⁵	Mixed P value⁶
Parameters	Con¹	IM²	OM³	HOM⁴	Con¹	IM²	OM³	HOM⁴	SEM⁵	Temperature	Minerals	T × M
Glucose, mg/dL	69.25	68.75	70.88	68.50	65.75	67.00	67.13	68.75	4.661	0.532	0.989	0.974
BUN, mg/dL	6.38	6.88	7.13	6.25	6.13	7.25	6.25	6.38	1.127	0.847	0.882	0.951
PHOS, mg/dL	7.55	7.55	7.40	7.41	7.75	7.85	7.35	7.61	0.273	0.438	0.683	0.941
Ca, mg/dL	9.24	9.18	9.26	9.08	9.35	9.21	9.43	9.24	0.159	0.312	0.640	0.977
Mg, mg/dL	2.00	2.01	1.95	2.03	2.01	2.01	2.06	2.01	0.033	0.277	0.989	0.270
Total P, g/dL	6.81	6.76	6.65	6.69	6.84	6.76	6.90	6.79	0.126	0.308	0.918	0.761
AST, U/L	81.00	80.50	81.50	82.50	83.63	87.75	88.00	87.75	3.320	0.046	0.871	0.925
TBIL, mg/dL	0.25	0.30	0.31	0.24	0.21	0.25	0.30	0.21	0.022	0.071	0.006	0.869
CHOL, mg/dL	78.25	72.63	73.13	75.00	88.13	75.88	84.88	81.75	7.930	0.198	0.771	0.961
Iron, μg/dL	137.50	136.75	140.00	146.75	144.00	145.75	141.88	142.75	7.728	0.573	0.956	0.869
Zn, μg/dL	97.00	100.00	95.63	102.38	100.63	103.38	106.00	106.13	4.764	0.186	0.798	0.901
Se, μg/dL	67.00	74.38	73.38	90.38	70.75	74.75	77.25	102.00	3.198	0.047	< 0.001	0.395

¹Con: Basal diet (without mineral supplementation)
²IM: Basal diet + inorganic minerals supplementation
³OM: Basal diet + organic minerals supplementation
⁴HOM: Basal diet + higher organic minerals supplementation
⁵Standard error of means
⁶P value obtained from the mixed procedure of SAS with temperature effect (B), mineral effect (M), and interaction effect between temperature and minerals (T × M). A P value less than 0.05 indicates that the results are statistically significant

ISSN: 2049-1891