Table 1 Primer sequences used in real-time PCR
Genes | Accession | Forward primer (5'→3') | Reverse primer (5'→3') |
|---|---|---|---|
PBD-1 | NM_213838.1 | ACCGCCTCCTCCTTGTATTCCTC | ACCGCCTCCTCCTTGTATTCCTC |
PBD-2 | NM_214442.2 | ATTAACCTGCTTACGGGTCTTGGC | CCACTGTAACAGGTCCCTTCAATCC |
PBD-3 | XM_021074698.1 | TCTTCTTGTTCCTGATGCCTCTTCC | GCCACTCACAGAACAGCTACCTATC |
PBD-114 | NM_001129973.1 | GTACCTTGGTGGATCCTGAACGATG | AACGCCCTCTGAATGCAGCATATC |
MUC-1 | NM_001164021 | CATCATCGTCCTGGTCGTC | CATCATCGTCCTGGTCGTC |
MUC-2 | EF140874 | GTCCAGAAAGCCCAAGAT ACC | GTGACATCATCACTTCCTCTGAG |
MUC-4 | NM_001083931.1 | GGCAACAGACGTGATCTATGAC | AGCGGCTGGCTGAAAACT |
HO-1 | NM_001004027.1 | TACCGCTCCCGAATGAACAC | GTCACGGGAGTGGAGTCTTG |
NQO-1 | NM_001159613.1 | GCGAGGGTCTCTGGTCCTTA | ATCACAGGTAAACTGAAGGACCC |
SOD | NM_000454 | TCCATGTCCATCAGTTTGGA | CTGCCCAAGTCATCTGGTTT |
GSH-Px | NM_201397.2 | TGGGGAGATCCTGAATTG | GATAAACTTGGGGTCGGT |
TNF-α | NM_214022.1 | CCACCAACGTTTTCCTCACT | TAGTCGGGCAGGTTGATCTC |
IL-1β | NM_214055.1 | CCAAAGAGGGACATGGAGAA | TTATATCTTGGCGGCCTTTG |
IL-6 | AF518322.1 | TGGCTACTGCCTTCCCTACC | AGAGCCTGCATCAGCTCAGT |
IL-12 | NC_010458.4 | CAACCCTGTGCCTTAGCAGT | AGAGCCTGCATCAGCTCAGT |
TGF-β | NM_214015.1 | GAAGCGCATCGAGGCCATTC | GGCTCCGGTTCGACACTTTC |
TLR4 | NM_001113039.1 | CAACCCTGTGCCTTAGCAGT | AGAGCCTGCATCAGCTCAGT |
MyD88 | NM_001099923 | CGCATGGTGGTGGTTGTT | GCCTTCTTCATCGCCTTGTATTT |
ERK | XM_021088019.1 | CAGTCTCTGCCCTCCAAGAC | GGGTAGATCATCCAGCTCCA |
JNK | XM_001929166.6 | TGGATGAAAGGGAACACACA | ATGATGACGATGGATGCTGA |
P38 | XM_021091323.1 | CCCTGAGGTTCTAGCGAAGA | TCTCATCGTAGGGCTCTGCT |
NF-κB | NM_001048232.1 | CTCGCACAAGGAGACATGAA | ACTCAGCCGGAAGGCATTAT |
β-actin | XM_021086047.1 | GATCTGGCACCACACCTTCTACAAC | RTCATCTTCTCACGGTTGGCTTTGG |