Fig. 8

Inhibition of autophagy alleviates the inflammatory response induced by S. maltophilia. MMECs were pretreated with autophagy inhibitor 3-MA, and then added with S. maltophilia for 6 h before being collected. A The autophagic level of MMECs was significantly reduced by 3-MA pretreatment. The decrease of p62 and the increase of LC3 II were significantly alleviated. B The destruction of the tight junction proteins Occludin and Claudin-3 in MMECs was significantly alleviated by 3-MA pretreatment. C and D Expression of inflammatory cytokines TNF-α and IL-1β in MMECs. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 indicate significance from each group. ns, no significance