Fig. 6

S. maltophilia significantly activated calcium, AMPK, mTOR, and autophagy signalling in MMECs. MMECs were collected after 6 h of treatment in a medium containing 3 × 10², 6 × 10², 1.2 × 10² CFU/mL of S. maltophilia. A Calcium concentration was measured by Flou-4 fluorescence probe. B ROS level was measured by C11-BODIPY fluorescent probe. C Level changes of AMPK signaling pathway. The p-AMPK level was significantly increased. D Level changes mTOR signaling pathways in MMECs. The p-mTOR level was significantly decreased. E The autophagic level of MMECs was significantly increased by S. maltophilia treatment. F The expression of p-p38 and p-p65 in the p/38MAPK and NF-κB signal pathways of MMECs was significantly increased under the stimulation of S. maltophilia in a concentration gradient. G The tight junction proteins Occludin and Claudin-3 in MMECs were significantly reduced. H and I Expression of inflammatory cytokines TNF-α and IL-1β in MMECs. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. ^*P < 0.05, ^**P < 0.01 and ^***P< 0.001 indicate significance from each group. ns, no significance