Fig. 4

Ingested S. maltophilia migrates from the gut to the mammary gland. The S. maltophilia were labeled with sf-GFP protein and then gavaged to mice for a week. The organs and blood of the mice were collected and the bacterial location was tracked. A The presence of sf-GFP gene fragments in mammary gland, breast lymph node, mesenteric lymph node, spleen, lung and serum was identified by agarose gel. B Western blot was used to detect the expression of sf-GFP protein in mammary gland. C Observe whether there are green fluorescent bacteria in mammary culture and mammary paraffin section. D After DAPI staining the nuclei of paraffin sections of mammary gland, observe the bacteria that emit green fluorescence. E The abundance of S. maltophilia in the mammary gland of untreated group and gavage group. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 indicate significance from each group. ns, no significance