Fig. 3
From: Regulation of serotonin production by specific microbes from piglet gut
The bacterial supernatants promote 5-HT synthesis in IPEC-J2 cells. A The effect of 1%, 5%, and 10% bacterial supernatant on cell viability. The effect of 10% bacterial supernatant on TPH1 (B), IDO1 (C), and SERT (D) mRNA expression. The effect of 10% bacterial supernatant on cell supernatant (E) and intracellular (F) 5-HT concentration. All values are mean ± SEM. n = 4–6 per group. The one-way ANOVA and multiple comparisons in Fisher’s LSD test were performed while asterisks mean statistically significant difference: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. TPH1: Tryptophan hydroxylase 1; IDO1: Indoleamine 2,3-dioxygenase 1; SERT: Serotonin reuptake transporter