Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation

Table 1 Deep sequencing results for mutation rates of double target genes in individual calves

ID	Beef		Dairy		Sex	Age
ID	MSTN	PRNP	MSTN	BLG	Sex	Age
#1	99.3%	13.7%			Male	10 months
#2	-	-			Male	-
#3	-	-			Female	-
#4	-	-			Male	-
#5^*			99.6%	100%	Female	Died due to infection
#6			4.6%	48.0%	Female	10 months
#7^**			-	-	Female	Died after birth
#8			0.9%	0%	Male	-
#9			34.9%	97.6%	Female	8 months
#10			50.3%	55.3%	Male	8 months

^*#5 was died and diagnosed as bacterial infection; ^**After autopsy, there is no finding any pathological infection and congenital defects. #1–#4: Beef cattle (MSTN/PRNP), #5–#10: Dairy cattle (MSTN/BLG), values below 0.1% were not presented. If there were various pattern, ratio of mutation pattern was presented

ISSN: 2049-1891