Fig. 1
From: Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation
In vitro production of double gene edited embryo via RNP electroporation. A MSTN/PRNP double gene edited embryos. B T7E1 assay results for mutation of MSTN (a) and PRNP (b) in MSTN/PRNP double gene edited embryos (lane 2–7). C MSTN/BLG double gene edited embryos. D T7E1 assay results for mutation of MSTN (a) and BLG (b) in MSTN/BLG double gene edited embryos (lane 2–6). M = 1 kb ladder, lane 1 = Wild type, N = negative control, P = positive control, red asterisk = lane with T7E1 positive results