Table 2 The primers for RT-qPCR assays
Target genes | Forward primer (5′→3) | Reverse primer (5′→3′) |
|---|---|---|
β-actin | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA |
SREBP-1c | CCCGAGGGAGACCATCTACA | GGTACTCCAACGCATCCGAA |
FAS | CCAACGATTACCCGTCTCAA | CAGGCTCTGTATGCTGTCCAA |
SCD1 | CCAGCGGAGATACTACAAGCC | CCGATTGCCAAACATGTGAGC |
GPAT1 | TCCATCGAGACCTAATGATAC | TAGACATCACAGCACAGGAC |
ChREBP | GATGAGCACCGCAAACCAGAGG | TCGGAGCCGCTTCTTGTAGTAGG |
HMGCR | CATAGGTGGCTACAACG | TACGCTCCATCAAAGTG |
LXRα | CAAAGGGAATGAATGAGC | AGCCGAAGGGCAAACAC |
PPARγ | GCAGGAACAGAACAAAGAAG | TGCCAGGTCACTGTCATCTA |
PPARα | TGTGGAGATCGTCCTGGTCT | CGTCAGGATGGTTGGTTTGC |
FXR | AGTAGAAGCCATGTTCCTCCGTT | GCAGTGCATATTCCTCCTGTGTC |
ABCA1 | TCAATCACCCGCTCAACT | CTGGCAGGAACAAAGGAC |
ACOX1 | ATGTCACGTTCACCCCATCC | AGGTAGGAGACCATGCCAGT |
CPT1 | TCGTCTTGCCATGACTGGTG | GCTGTGGTGTCTGACTCGTT |
L-FABP | GAAGAGTGTGAGATGGAGCTGCTG | GGTGATGGTGTCTCCGTTGAGTTC |
TNF-α | GGATGGATGGAGGTGAAAGTAG | TGATCCTGAAGAGGAGAGAGAA |
IL-6 | CGCCCAGAAATCCCTCCTC | AGGCACTGAAACTCCTGGTC |
TLR4 | CCACTATTCGGTTGGTGGAC3 | ACAGCTTCTCAGCAGGCAAT |
NF-κB | CTCTCCCAGCCCATCTATGA | CCTCAGCCCAGAAACGAAC |