Fig. 3
From: Punch-excised explants of bovine mammary gland to model early immune response to infection
Analysis of cell populations in teat explants after treatment with TLR-agonists. A Gating strategy set up to analyse leukocyte populations by flow cytometry. B–D Explants were treated with LPS (1 µg/mL) or LTA (10 µg/mL) for 24 h. Complete medium alone was used as control. Percentages of leukocytes (B), granulocytes (C) and macrophages (D). Box-plots show data from 4 independent replicates (4 animals, 1 teat/animal)