Fig. 2

Tissue integrity upon stimulation with TLR-agonists. A Evaluation of cytotoxicity by lactate dehydrogenase (LDH) release assay. Explants were treated with LPS (1 µg/mL) or LTA (10 µg/mL) for 24 h. Complete medium alone and DPBS (nutrient starvation) were used as negative and positive controls, respectively. Box-plot shows data from 4 independent replicates (4 animals, 1 teat/animal). B Representative photomicrograph of an explant stained with haematoxylin and eosin. Dashed rectangle highlights typical teat microscopic architecture. C–E Histological analysis of explants stimulated as described in A. Blue bar = 500 µm. Green bar = 50 µm. Black bars = 20 µm