Fig. 5

Raman spectroscopic in ovo sexing on d 3.5. A Preprocessed Raman spectra with mean female spectrum on top (red color) and mean male spectrum at the bottom (blue color) with the most prominent bands being indicated. B The mean male spectrum was subtracted from the mean female spectrum indicating the main spectral regions. C Opened shell to access the embryo's blood vessels [88]. The figure is reprinted with permission from ACS Publications