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Table 3 Histochemical results and affinity for different lectins in the anterior-mid intestinal mucosa of gilthead seabream (Sparus aurata) fed experimental low fishmeal diets containing Debaryomyces hansenii (1.1%, 17.2 × 105 CFU; Yeast diet) or devoid of the probiotic (Control diet)

From: Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish

 

Control diet

Yeast diet

General histochemistry

 Neutral glycoproteins

1–2

1–2

 Carboxylated glycoproteins

1–3

1–4

 Weakly inonised sulphated glycoconjugates

2–3

3

 Strongly ionised sulphated glycoconjugates

2–3

2–3

Lectin histochemistry

 ConA (Man/Glu)

0–1

0–1

 WGA (βGlcNAc > NeuNAc/sialic acids/NANA)

2–3

1–2

 SNA (Neur5Acα2; sialic acids/NANA)

0–1

0–1

 SBA (α/β GalNAc)

0–3

2–3

 UEA-I (L-Fuc)

0

0

  1. Semi-quantitative assessment scoring based on color intensity scores: 0, negative (non-detected); 1, weak; 2, moderate; 3, intense; 4, very intense