Fig. 3
Histochemical localization of glycoconjugates containing sugar residues in the anterior-mid intestine of gilthead seabream (Sparus aurata) fed experimental low fishmeal diets containing Debaryomyces hansenii (1.1%, 17.2 × 105 CFU; Yeast diet) or devoid of the probiotic (Control diet). Presence of glycoconjugates containing α/β-N-acetyl-D-galactosamine in mucous cells of S. aurata fed a Control (a) or a Yeast diet (b). Results denote a moderate increase in affinity for the SBA lectin in the mucous cells and in the intestinal epithelium from Yeast diet. Glycoconjugates containing N-acetyl-D-glucosamine and/or N-acetylneuraminic acid residues in mucous cells of S. aurata fed the Control or the Yeast diet (d). Results denote a moderate decrease in affinity for the WGA lectin in the mucous cells from the Yeast diet. Histochemical detection of glycoconjugates containing N-acetylneuraminic acid/sialic acid residues in the intestine from the Control (e) or Yeast (f) groups. Note the decrease in affinity for the SNA lectin in the anterior-mid intestinal epithelium of S. aurata fed the Yeast diet. Glycoconjugates containing a-mannose/a-glucose residues in anterior-mid intestine from S. aurata fed Control (g) or Yeast diets (h). Observe the increase in affinity for the ConA lectin in the intestinal epithelium of S. aurata fed the Diet Y. Abbreviations: e: epithelium; mc: mucous cells. Scale bar = 50 μm