Fig. 6

The expression analysis of SOSTDC1 gene. A The relative expression of SOSTDC1 at different tissues of newborn stage lambs, n = 3, the different letters indicate significant difference among tissues (P < 0.05). B The relative expression of SOSTDC1 mRNA between coarse and fine wool lambs skin tissue, ALC lambs: the ancestral-like coarse lambs in this study (n = 4), Tan lambs: Tan sheep breed at one month of age (n = 4), Ujimqin lambs: Ujimqin sheep breed at one month of age (n = 4), Merino lambs: Aohan fine wool sheep at one month of age (n = 4), MF lambs: The half/full siblings of ALC lambs with fine wool (n = 4). C The amino acid sequence alignment of SOSTDC1 protein in mice, sheep and humans using DANMAN software. D Western-blot analysis of SOSTDC1 protein skin tissues of coarse and fine wool lambs. β-actin was used as housekeeping protein. ALC: the ancestral-like coarse lamb, Tan: Tan lamb, Ujimqin: Ujimqin lamb, MF: The half/full siblings of ALC lambs with fine wool, Merino-1 and Merino-2 indicate Aohan fine wool lamb. E Gray-scale analysis of Western-blot result in D using image J. ^*0.01 < P < 0.05. F SOSTDC1 protein was detected in primary wool follicle of ALC lambs by immunofluorescence. The left, Immunofluorescent staining for SOSTDC1. The middle, Nuclei were stained with DAPI. The right, Merged immunofluorescence images using SOSTDC1 (red) and DAPI–stained nuclei (blue)