Fig. 4

Analysis of differentially expressed genes at different developmental stages of skin tissue between coarse and fine wool breeds using transcriptome public data. A RNA-seq data analysis workflow of coarse and fine wool breeds. (I) Fastq data check and quality control. (II) Reads alignment and count. (III) Gene differential expression analysis. B PCA analysis of all expressed genes for sheep of different breeds and different periods. C The bar-plot of TPM mean for DNMT3A, ARID4A, BRCA1, CTCF gene between coarse and fine wool breeds at three stages. ^*0.01 < P < 0.05, ^***P < 0.001. D The bar-plot of TPM mean for METTL3, METTL14, FTO, ALKBH5, YTHDF1, YTHDF2 and HNRNPA2B1 gene between coarse and fine wool breed at three stages. ^*0.01 < P < 0.05, ^***P < 0.001. E The key epigenetic regulators expression in ALC wool lamb skin