Fig. 5

Effects of gga-miR-301b-3p on the proliferation of chicken abdominal preadipocytes. A Tissue expression pattern of gga-miR-301b-3p in chickens with high and low abdominal fat percentage. B Expression pattern of gga-miR-301b-3p during the proliferation of chicken abdominal preadipocytes. C CCK8 assay of chicken abdominal preadipocytes transfected with miR-301b-3p agomir and miR-301b-3p agomir NC. D CCK8 assay of chicken abdominal preadipocytes transfected with miR-301b-3p antagomir and miR-301b-3p antagomir NC. E Proliferation of chicken abdominal preadipocytes determined by EdU after 48 h of transfection with miR-301b-3p agomir and miR-301b-3p agomir NC. F Histogram showing the proportion of gga-miR-301b-3p-overexpressing EdU-positive cells using ImageJ. G Proliferation of chicken abdominal preadipocytes determined by EdU after 48 h of transfection with miR-301b-3p antagomir and miR-301b-3p antagomir NC. H Histogram showing the proportion of gga-miR-301b-3p-knockdown EdU-positive cells using ImageJ. I and J Flow-cytometric cell cycle analysis of chicken abdominal preadipocytes after 48 h of transfection with miR-301b-3p agomir and miR-301b-3p agomir NC. K and L Flow-cytometric cell cycle analysis of chicken abdominal preadipocytes after 48 h of transfection with miR-301b-3p antagomir and miR-301b-3p antagomir NC. ^*P < 0.05, ^**P < 0.01