Fig. 4

circDOCK7 functions as a gga-miR-301b-3p sponge. A and B Relative expression of gga-miR-301b-3p in proliferative and differentiated chicken abdominal preadipocytes upon circDOCK7 overexpression and knockdown. C and D Relative expression of the ACSL1 gene in proliferative and differentiated chicken abdominal preadipocytes upon circDOCK7 overexpression and knockdown. E Construction of dual-luciferase reporter vectors for the validation of gga-miR-301b-3p binding to circDOCK7. WT: wild-type vector; Mut: mutant vector; hRluc: Renilla luciferase; hluc + : firefly luciferase. F Validation of the interaction between gga-miR-301b-3p and circDOCK7 via a dual-luciferase reporter assay in 293 T cells and DF1 cells. G Detection of the combination of circDOCK7 and gga-miR-301b-3p by an RNA pull-down assay. H qRT-PCR analysis of gga-miR-301b-3p expression pulled down by the circDOCK7 probe. ^*P < 0.05, ^**P < 0.01