Fig. 2

Effects of circDOCK7 knockdown on the proliferation of chicken abdominal preadipocytes. A CCK8 assay of chicken abdominal preadipocytes transfected with siNC and sicircDOCK7 at 6, 24, 48, 72 and 96 h post-transfection. B Detection of circDOCK7 knockdown in chicken abdominal preadipocytes 48 h post-transfection with sicircDOCK7. The relative gene expression levels are shown as fold changes compared with that in the siNC group. The same below. C Proliferation of chicken abdominal preadipocytes determined by 5-ethynyl-2′-deoxyuridine (EdU) after 48 h of transfection with sicircDOCK7. D Histogram showing the proportion of EdU-positive cells using ImageJ. E and F Cell cycle analysis of chicken abdominal preadipocytes after 48 h transfection with sicircDOCK7 using flow cytometry. ^*P < 0.05, ^**P < 0.01