Fig. 11

circDOCK7 augments adipogenesis in a gga-miR-301b-3p-dependent manner. A Relative expression of the ACSL1 gene in proliferative chicken abdominal preadipocytes co-transfected with sicircDOCK7 and miR-301b-3p antagomir. The relative gene expression levels are shown as fold changes compared with that in the siNC + antagomir NC group. B–D CCK8 and EdU staining assays in proliferative chicken abdominal preadipocytes co-transfected with sicircDOCK7 and miR-301b-3p antagomir. E Relative expression of the ACSL1 gene in differentiated chicken abdominal preadipocytes co-transfected with circDOCK7 overexpression vector and miR-301b-3p agomir. The relative gene expression levels are shown as fold changes compared with that in the pLC5-ciR + agomir NC group. F–H Intracellular lipid droplet accumulation and triglyceride and acetyl-CoA levels in differentiated chicken abdominal adipocytes co-transfected with circDOCK7 overexpression vector and miR-301b-3p agomir. ^*P < 0.05, ^**P < 0.01. ^a–cMeans with different lowercase letters indicate significant difference (P < 0.05)