Enzymatically prepared alginate oligosaccharides improve broiler chicken growth performance by modulating the gut microbiota and growth hormone signals

Table 1 Basal diet composition (as-fed basis)

Ingredient, %	d 1–21	d 22–42
Corn	56.05	57.35
Soybean meal (CP > 44%)	35.67	32.21
Soy oil	2.60	4.30
Corn gluten meal (CP > 51.3%)	2.00	2.60
Limestone	0.80	0.99
Dicalcium phosphate	1.90	1.44
Salt	0.30	0.30
Methionine (99%, DL-form)	0.22	0.12
Choline (50%)	0.20	0.30
Lysine-HCl (78%, L-form)	-	0.13
Vitamin premix^a	0.02	0.03
Mineral premix^b	0.20	0.20
Ethoxyquin (66%)	0.03	0.03
AOS premix^c	0.00	0.00
Total	100.00	100.00
Calculated composition^d, %
Crude protein	21.54	20.00
Metabolizable energy, kcal/kg	2,970.00	3,070.00
Calcium	1.00	0.90
Available phosphorous	0.51	0.40
Lysine	1.21	1.10

^aProvided per kg of diet: vitamin premix (1 kg) contained the following: vitamin A, 50 MIU; vitamin D₃, 12 MIU; vitamin K₃, 10 g; vitamin B₁, 10 g; vitamin B₂, 32 g; vitamin B₁₂, 0.1 g; vitamin E, 0.2 MIU; biotin, 0.5 g; folic acid, 5 g; pantothenic acid, 50 g; niacin, 150 g
^bProvided per kg of diet: copper, 4 g; zinc, 90 g; iron, 38 g; manganese, 46.48 g; selenium, 0.1 g; iodine, 0.16 g; cobalt, 0.25 g
^cAlginate oligosaccharide premix was added at the expense of corn to supply 0, 100, 200 or 400 mg AOS/kg diet
^dCalculated value based on the analyzed data for the experimental diets

ISSN: 2049-1891