Table 3 Primer sequences for real-time quantitative PCR
From: CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9
Gene name | Forward 5'→3' | Reverse 5'→3' | Size, bp | Accession No. |
|---|---|---|---|---|
GAPDH | AGGTCGGAGTGAACGGATTTG | CCATGTAGTGGAGGTCAATGAAG | 117 | NM_001206359.1 |
CLOCK | GCCAGCAGCATGGTCCAGATTC | TCTGTCTGTCCTGAGGGAACGC | 88 | XM_021101324.1 |
CCNB1 | AATCCCTTCTTGTGGTTA | CTTAGATGTGGCATACTTG | 104 | NM_001170768.1 |
CCND1 | TACACCGACAACTCCATCCG | GAGGGCGGGTTGGAAATGAA | 224 | XM_021082686.1 |
CCNE1 | AGAAGGAAAGGGATGCGAAGG | CCAAGGCTGATTGCCACACT | 173 | XM_005653265.2 |
CDK1 | CAGCTCGCTACTCAACTCCA | GAGTGCCCAAAGCTCTGAAA | 135 | NM_001159304.2 |
CDK4 | AAGTGGTGGGACAGTCAAGC | ACCACCACAGGTGTAAGTGC | 81 | NM_001123097.1 |
ASB9 | ACCTGGGCACACCTTTATATTTGGC | GGTTCACACTCGCTCCTGATTCC | 87 | NM_001243703.1 |