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Table 2 The reaction conditions of RT-qPCR

From: CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9

Stage

Cycle number

Temperature

Time

Predenaturation

1

95 °C

300 s

Denaturation

40

95 °C

10 s

Annealing

40

60 °C

30 s

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891