Table 3 Primer sequences used in qRT- PCR analyses
Gene | Primers | Sequence (5’→3’) | Gene Bank NO |
|---|---|---|---|
ef1α | F | ATCAAGAAGATCGGCTACAACCCT | KJ123689.1 |
R | ATCCCTTGAACCAGCTCATCTTGT | Â | |
β-actin | F | AGCCTTCCTTCCTTGGTATGGAAT | KJ126772.1 |
R | TGTTGGCGTACAGGTCCTTACG | Â | |
muc2 | F | ACTACAACTCCCCCTCCTCC | XM_025902524.1 |
R | CCCACCCTTCACATACACAGTC | Â | |
nfkb | F | CGACCACTACCTACACGCTC | XM_019363515.2 |
R | GATGTCGTTTGAGGCATCGC | Â | |
il1β | F | GAGCACAGAATTCCAGGATGAAAG | XM_019365842.2 |
R | TGAACTGAGGTGGTCCAGCTGT | Â | |
perk | F | GATGTTTCAGGGGCAGCTCT | XM_003447769.5 |
R | CGTCGTGGGAGAACTTGTCA | Â | |
eif2a | F | AGAGCTTCTTCAAGGCCGAC | XM_005462748.4 |
R | TTGGGCCGTTCTTCGATAGC | Â | |
atf4 | F | CATTGTTCCTCGTTCCTGGG | XM_005464867.4 |
R | CAAAAGCATCATCTGCTTTGCCA | Â | |
casp3 | F | GGAGTGGACGATACAGACGCAAA | NM_001282894.1 |
R | TGAAGCTGTGTGACTGGGGCTT | Â |