Xylanase improves the intestinal barrier function of Nile tilapia (Oreochromis niloticus) fed with soybean (Glycine max) meal

Table 1 The diet formulation for the first experiment

Ingredients, g/kg	SM	SMC
Fish meal (670 g/kg protein)	75	75
Casein (900 g/kg protein)	120	120
Soybean meal (460 g/kg protein)	400	400
Corn starch	300	300
Fish oil	13.25	13.25
Soybean oil	27	27
Vitamin premix¹	12	12
Mineral premix²	12	12
Carboxymethyl cellulose (CMC)	10	10
Cellulose	8.5	8.5
Butylated hydroxytoluene	0.25	0.25
Choline chloride	5	5
Ca(H₂PO₄)₂	15	15
Dimethyl-beta-propiothetin	2	2
Xylanase	0	3,000U
Total quantity	1,000	1,000
Crude lipid	4.93	4.96
Crude protein	33.8	33.4

¹Mixed vitamin (g/kg): Inositol 25 g, Cholin 100 g, Vitamin B₁₂ 5 g, Niacin 25 g, Vitamin B₆ 5 g, Folic acid 1 g, Vitamin B₂ 5 g, Biotin 0.25 g, Vitamin B₁ 5 g, Vitamin C 10 g, Vitamin K₃ 1 g, Vitamin A 0.15 g, Vitamin E 2.5 g, Pantothenic acid 10 g, Vitamin D₃ 0.00125 g
²Mixed minerals (g/kg): NaSeO₃ 0.02 g, MgSO₄·7H₂O 147.4 g, NH₄ molybdate 0.06 g, CoCl₂·6H₂O 0.08 g, NaCl 49.8 g, KI 0.16 g, Fe (II) gluconate 10.9 g, CuSO₄·5H₂O 0.62 g, ZnSO₄·7H₂O 4.67 g, MnSO₄·H₂O 3.12 g

ISSN: 2049-1891