Fig. 3

Effects of dietary supplementation with xylanase on endoplasmic reticulum (ER) stress in Nile tilapia. A Pathways changed in comparison SM vs. SMC (n = 4). Gene level of B Protein kinase RNA-like endoplasmic reticulum kinase (perk), C Initiation Factor 2 alpha (eif2α), D Activating transcription factor 4 (atf4) (n = 6), E Western blot analysis of intestinal glucose regulatory protein 78 (GRP78) and C/EBP homologous protein (CHOP) (n = 3), F Quantitation of the levels of GRP78 and CHOP normalised to that of α-tublin (n = 3). Data was expressed as mean ± SEM. SM, fish fed with soybean diet; SMC, fish fed with soybean diet supplemented with 3,000 U/kg xylanase. The significant differences between two group were presented at P < 0.05 (*), P < 0.01 (**)