Effects of dietary coenzyme Q10 supplementation during gestation on the embryonic survival and reproductive performance of high-parity sows

Table 2 Primers for target genes in Exp. 1

Genes	Accession number	Primers (5' → 3')	Tm, °C	Product size, bp
COQ-2	XM_003129357.5	F: CACCTACCCGCTAATGAAA	53.5	131
COQ-2	XM_003129357.5	R: AAGAGGAAGGCAAACAGAT	53.5	131
COQ-3	XM_021090298.1	F: CGATGTGGCGAGGCTGTAAG	61.1	133
COQ-3	XM_021090298.1	R: CGCGACCTCCAGACACACTC	61.1	133
COQ-6	XM_001929106.5	F: TGATTGGTGCTGATGGTC	52.8	118
COQ-6	XM_001929106.5	R: TCCGTGGGCTCTGATAAA	52.8	118
PDSS-1	XM_005668142.3	F: CAGATGGGCAAACCGACCT	59.06	80
PDSS-1	XM_005668142.3	R: GGAAACTGCTGACAAGCGAA	59.06	80
PGC1α	NM_213963	F: CCCGAAACAGTAGCAGAGACAAG	60.5	111
PGC1α	NM_213963	R: CTGGGGTCAGAGGAAGAGATAAAG	60.5	111
CcOX I	AJ950517.1	F: ATTATCCTGACGCATACACAGCA	60.2	127
CcOX I	AJ950517.1	R: GCAGATACTTCTCGTTTTGATGC	60.2	127
CcOX IV	AK233334.1	F: CCAAGTGGGACTACGACAAGAAC	60.0	131
CcOX IV	AK233334.1	R: CCTGCTCGTTTATTAGCACTGG	60.0	131
CcOX V	AY786556.1	F: ATCTGGAGGTGGTGTTCCTACTG	61.2	160
CcOX V	AY786556.1	R: GTTGGTGATGGAGGGGACTAAA	61.2	160
ACTB	XM_003124280.3	F: TCTGGCACCACACCTTCT
ACTB	XM_003124280.3	R: TGATCTGGGTCATCTTCTCAC

COQ-2 Coenzyme Q2, COQ-3 Coenzyme Q3, COQ-6 Coenzyme Q6, PDSS-1 Decaprenyl diphosphate synthase subunit 1, PGC1α Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, CcOX Cytochrome c oxidase

ISSN: 2049-1891