Chronic heat stress induces renal fibrosis and mitochondrial dysfunction in laying hens

Table 3 Sequences of the primers used for mtDNA analysis

Gene¹	Primer sequences² (5'→3')	Accession no	Source or reference of primer sequences
ND4³	F: CGCAGGCTCCATACTACTCG	NC_040970.1	[38]
ND4³	R: TTAGGGCACCTCATAGGGCT	NC_040970.1	[38]
COX1³	F: CCATACTACTTACCGACCGCAACC	NC_040970.1	[24]
COX1³	R: GTGTCTACGTCCATTCCGACTGTG	NC_040970.1	[24]
ATP6³	F: ATTCTCAAGCCCCTGCCTAC	NC_053523.1	[24]
ATP6³	R: TCAGAGTTGGATGGTGGAGAGG	NC_053523.1	[24]
ND6³	F: TAACAACAAACCTCACCCAGCC	NC_053523.1	[24]
ND6³	R: GTGTGTCTTTTGCTCGGTTGGA	NC_053523.1	[24]
β-actin⁴	F: ATCCGGACCCTCCATTGTC	NM_205518.1	[24]
β-actin⁴	R: AGCCATGCCAATCTCGTCTT	NM_205518.1	[24]

¹ND4 NADH dehydrogenase subunit 4, COX1 Mitochondrial cytochrome c oxidase1, ATP6 ATP synthase F0 subunit 6, ND6 NADH dehydrogenase subunit 6
²F Forward, R Reverse
³Genes were used to amplify fragment of mitochondrial DNA
⁴Genes were used to amplify fragment of cDNA

ISSN: 2049-1891