Chronic heat stress induces renal fibrosis and mitochondrial dysfunction in laying hens

Nanto-Hara, Fumika; Yamazaki, Makoto; Murakami, Hitoshi; Ohtsu, Haruhiko

doi:10.1186/s40104-023-00878-5

Journal of Animal Science and Biotechnology

Table 2 Sequences of the primers used for quantitative real-time PCR

From: Chronic heat stress induces renal fibrosis and mitochondrial dysfunction in laying hens

Gene¹	Primer sequences² (5'→3')	Accession no	Source or reference of primer sequences
COL1A1	F: ACCTCAGCAAGAACCCCAAG	XM_025144131.2	[34]
COL1A1	R: CTCACCGCCGTACTCAAACT	XM_025144131.2	[34]
COL1A2	F: GCGGTTTCTACTGGATTGA	NM_001079714.2	[34]
COL1A2	R: AGCGAGACGGCTTATTTG	NM_001079714.2	[34]
αSMA	F: AAGCACCACTGAATCCCAAAG	NM_001031229.1	[34]
αSMA	R: CCAGAGTCAAGCACAATCCCT	NM_001031229.1	[34]
TGF-β	F: GCAAACTGCGTCTGACCG	NM_001318456.1	[34]
TGF-β	R: ACGAAGAAGATGCTGTGGC	NM_001318456.1	[34]
MDA5	F: CGAATGAAAACCTGGGACAG	AB371640	[35]
MDA5	R: TGGTTTTGCCACTGCCTGTA	AB371640	[35]
STING	F: CGGCTGTGACATCTGGGAT	KP893157	[35]
STING	R: CCCGAGTCAGGATGGTCTC	KP893157	[35]
IRF7	F: ACAACGCCAGGAAGGATGTC	NM_205372	[35]
IRF7	R: CCAGCAGCATGAACATGTGA	NM_205372	[35]
MAVS	F: GAACGCAAACCACCTTCAAC	NM_001012893	[35]
MAVS	R: CCAGGAGCAGCACTCAAATC	NM_001012893	[35]
IFN-β	F: TTGCCCACAACAAGACGTGA	GU119897/AY974089	[35]
IFN-β	R: GTGTGCGGTCAATCCAGTGT	GU119897/AY974089	[35]
IFN-γ	F: GTCAAAGCCGCACATCAAAC	NM_205149.1	[35]
IFN-γ	R: GGCTTTGCGCTGGATTCTC	NM_205149.1	[35]
IL-1β	F: GGCCTGAGTCATGCATCGTT	NM_204524.1	[35]
IL-1β	R: ATAAATACCTCCACCCCGACAA	NM_204524.1	[35]
IL-8	F: GGCTTGCTAGGGGAAATGA	AJ009800	[31]
IL-8	R: AGCTGACTCTGACTAGGAAACTGT	AJ009800	[31]
CCL2	F: GGCAGACTACTACGAGACCAACAG	L34553	[31]
CCL2	R: ACGGCCCTTCCTGGTGAT	L34553	[31]
CCL4	F: CTTCACCTACATCTCCCGGC	NM_001030360	[36]
CCL4	R: CTGTACCCAGTCGTTCTCGG	NM_001030360	[36]
CCL20	F: AGGCAGCGAAGGAGCAC	NM_204438	[36]
CCL20	R: GCAGAGAAGCCAAAATCAAAC	NM_204438	[36]
18S rRNA	F: TCAGATACCGTCGTAGTTCC	HQ873432.1	[37]
18S rRNA	R: TTCCGTCAATTCCTTTAAGTT	HQ873432.1	[37]

¹COL1A1 Collagen type I alpha 1, COL1A2 Collagen type I alpha 2, αSMA α-smooth muscle actin, TGF-β Transforming growth factor-β, MDA5 Melanoma differentiation-associated gene 5, STING Stimulator of interferon genes, IRF7 Type I interferon regulatory factor 7, MAVS Mitochondrial antiviral signaling, IFN- Type I interferon-, IL- Interleukin-, CCL C–C motif chemokine ligands, 18S rRNA 18S ribosomal RNA
²F Forward, R Reverse

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ISSN: 2049-1891

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