Fig. 2

Metabolomic characterization of chicken AIL population. A Venn diagram of metabolites identified in the four detection modes. The same metabolites were detected by multiple modes with a count of 1. B Pie chart showing the percentage of each type of metabolite detected across all four modes. C Distribution of the values of the coefficient of variation (CV) across the four detection modes, with the mean CV indicated by a horizontal line. D Distribution of the broad-sense heritability (h²) of metabolites across the four detection modes, with the dashed red line representing h² = 0.2. E Heat map displaying paired Pearson's correlations (r) between metabolites detected under the T3 modes, with metabolites classified according to a hierarchical clustering analysis based on correlations. Metabolites included in heat map had an r > 0.8, P < 0.05. F Heat map displaying paired Pearson’s correlations (r) between metabolites detected under the Amide modes, with metabolites classified according to a hierarchical clustering analysis based on correlations. Metabolites included in heat map had an r > 0.8, P < 0.05