Effects of tannic acid on the immunity and intestinal health of broiler chickens with necrotic enteritis infection

Table 7 Effects of tannic acid on the serum immune indices of chickens with NE

	NC	PC	PTA1	PTA2	PTA3	PTA4	P-value¹	L²	Q²
d 28
IgA, mg/mL	0.28 ± 0.04	0.34 ± 0.05	0.29 ± 0.03	0.30 ± 0.02	0.33 ± 0.03	0.28 ± 0.011	0.630	0.305	0.851
IgM, mg/mL	0.08 ± 0.01^c	0.16 ± 0.02^ab	0.15 ± 0.01^ab	0.18 ± 0.03^ab	0.21 ± 0.02^a	0.15 ± 0.01^b	0.001	0.807	0.163
Serum-specific IgY antibody, OD 450 nm	2.79 ± 0.05^b	2.92 ± 0.03^a	2.87 ± 0.02^ab	2.85 ± 0.05^ab	2.79 ± 0.04^b	2.78 ± 0.03^b	0.090	0.003	0.637
CRP, μg/mL	6.53 ± 0.76^c	13.97 ± 1.28^a	12.64 ± 1.20^ab	12.78 ± 0.70^ab	11.50 ± 0.63^ab	10.77 ± 1.04^b	< 0.001	0.024	0.952
MPO, pg/mL	697.06 ± 65.48^c	1,291.64 ± 99.90^a	1,305.64 ± 109.51^a	1,132.20 ± 86.60^ab	1,008.71 ± 103.05^b	968.49 ± 76.27^b	< 0.001	0.004	0.872
sIgA, μg/mg prot	30.42 ± 3.58^b	52.52 ± 8.21^a	34.88 ± 3.78^b	35.13 ± 4.43^b	28.96 ± 3.19^b	28.07 ± 2.76^b	0.008	0.001	0.149
d 35
IgA, mg/mL	0.38 ± 0.04	0.37 ± 0.05	0.36 ± 0.08	0.36 ± 0.08	0.35 ± 0.04	0.31 ± 0.03	0.972	0.497	0.737
IgM, mg/mL	0.08 ± 0.01	0.08 ± 0.02	0.07 ± 0.02	0.08 ± 0.04	0.06 ± 0.003	0.05 ± 0.05	0.903	0.332	0.724
Serum-specific IgY antibody, OD 450 nm	2.87 ± 0.04	2.88 ± 0.03	2.92 ± 0.02	2.88 ± 0.03	2.88 ± 0.02	2.90 ± 0.03	0.903	0.964	0.993
CRP, μg/mL	9.02 ± 0.65^c	14.00 ± 0.36^a	11.27 ± 0.66^b	10.16 ± 0.70^bc	9.48 ± 0.87^bc	8.59 ± 0.80^c	< 0.001	< 0.001	0.128
MPO, pg/mL	765.28 ± 50.28^c	1,570.16 ± 46.42^a	1,009.84 ± 60.30^b	973.55 ± 65.72^b	871.87 ± 53.66^bc	852.16 ± 57.78^bc	< 0.001	< 0.001	< 0.001
sIgA, μg/mg prot	33.02 ± 4.45	38.44 ± 4.10	36.82 ± 2.67	35.72 ± 4.90	31.56 ± 3.13	29.74 ± 1.27	0.531	0.046	0.792

n = 7. Data are presented as mean ± SEM. NC No tannic acid treatment or NE infection, PC NE infection + 0 mg/kg tannic acid, PTA1 NE infection + 250 mg/kg tannic acid, PTA2 NE infection + 500 mg/kg tannic acid, PTA3 NE infection + 750 mg/kg tannic acid, PTA4 NE infection + 1,000 mg/kg tannic acid, L Linear, Q Quadratic
¹Overall P-values obtained from ANOVA; ²P-values obtained using contrast trend analysis. ^a^–^cDifferent lower-case letters in the same column indicate significant differences (P < 0.05)

ISSN: 2049-1891