Effects of tannic acid on the immunity and intestinal health of broiler chickens with necrotic enteritis infection

Table 2 Sequences of the oligonucleotide primers used for quantitative reverse transcription-PCR^a

Gene	Primer sequences(5ʹ→ 3ʹ)	NCBI serial number
β-actin	F-CAACACAGTGCTGTCTGGTGGTAC	NM_205518.1
	R-CTCCTGCTTGCTGATCCACATCTG
Occludin	F-ACGGCAGCACCTACCTCAA	NM_205128.1
	R-GGGCGAAGAAGCAGATGAG
MUC2	F-TCCCCTGTTGAGGGAGAACTT	XM_040673077.1
	R-AGTGGTTGTACCTTCGGTGC
ZO-1	F-CTTCAGGTGTTTCTCTTCCTCCTC	XM_040680632.1
	R-CTGTGGTTTCATGGCTGGATC
PEPT1	F-TACGCATACTGTCACCATCA	NM_204365.2
	R-TCCTGAGAACGGACTGTAAT
SGLT1	F-GATGTGCGGATACCTGAAGC	NM_001293240.1
	R-AGGGATGCCAACATGACTGA
GLUT2	F-CCGCAGAAGGTGATAGAAGC	NM_205129.1
	R-ATTGTCCCTGGAGGTGTT
I-FABP	F-GAAGCAATGGGCGTGAATGTGATG	NM_001007923.1
	R-TTCGATGTCGATGGTACGGAAGTTG
IL-1β	F-ACTGGGCATCAAGGGCTA	XM_046931582.1
	R-GGTAGAAGATGAAGCGGGTC
IL-6	F-CGCCCAGAAATCCCTCCTC	NM_204628.2
	R-AGGCACTGAAACTCCTGGTC
TLR2	F-GATTGTGGACAACATCATTGACTC	NM_001161650.3
	R-AGAGCTGCTTTCAAGTTTTCCC
TGF-β	F-TCATCACCAGGACAGCGTTA	NM_001031045.3
	R-TGTGATGGAGCCATTCATGT

F Upstream primer, R Downstream primers, ZO-1 Zonula occludens-1, PEPT1 Peptide-transporter 1, SGLT1 Sodium glucose cotransporter 1, GLUT2 Glucose transporter 2, I-FABP Intestinal fatty acid binding protein, IL-1β Interleukin-1β, IL-6 Interleukin-6, TLR2 Toll like receptor 2, TGF-β Transforming growth factor-β
^aThe primers were synthesized by Shanghai Shenggong Biotechnology Co., Ltd.

ISSN: 2049-1891