Fig. 5

Over-expression with CLN8 promotes the differentiation of adipocytes. A and B CLN8^OE cell significantly promoted CLN8 mRNA and protein expression in ICPs. C and D mRNA levels of adipocyte genes PPARγ and FABP4 were analyzed with RT-PCR. E Representative images of CLN8^OE cells increased the lipid droplet formation by Oil red O staining on d 3. F Comparison of the lipid droplet content of CLN8^OE and CLN8^NC cells obtained by Oil red O staining and extraction methods. Data are shown as mean ± SD of three biological replicates. An independent sample t-test was used to analyze the statistical differences between groups. The level of significance was presented as ^*P < 0.05,  ^**P < 0.01, ^***P < 0.001