Fig. 4

The functional polymorphism SNPs decreased the transcriptional activity of CLN8. A Wild-type and mutant-type CLN8 plasmids were verified by direct sequencing. B Three luciferase reporter gene constructs were generated. They share identical backbone sequences except for the polymorphisms as left. Significantly lower luciferase activity of the mutant type (-A-T-C–C-G-) haplotype was observed when compared with the wild type (-T-C-A-A-C-) haplotype vectors in the ICP1 cell line. Data are shown as mean ± SD of three biological replicates. An independent sample t-test was used to analyze the statistical differences between groups. The level of significance was presented as ^*P < 0.05, ^**P < 0.01, ^***P < 0.001